Data/hora: 05/06/2024 14:08:30

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 28/06/2000
Data da última atualização: | 25/02/2019
Autoria: | GLORIA, F. J. M. da; MOURAO FILHO, F. de A. A.; MENDES, B. M. J.
Afiliação: | FERNANDA JANUZZI MENDES DA GLORIA, Universidade de São Paulo - USP/Escola Superior de Agricultura Luiz de Queiroz - ESALQ/Departamento de Produção Vegetal; FRANCISCO DE ASSIS ALVES MOURÃO FILHO, Universidade de São Paulo - USP/Escola Superior de Agricultura Luiz de Queiroz - ESALQ/Departamento de Produção Vegetal; BEATRIZ MADALENA JANUZZI MENDES, Universidade de São Paulo - USP/Centro de Energia Nuclear na Agricultura - CENA/Laborátorio de Biotecnologia de Plantas.
Título: | Plant regeneration from protoplast of brazilian citrus cultivars.
Ano de publicação: | 2000
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira, Brasília, DF, v. 35, n. 4, p. 727-732, abr. 2000.
Idioma: | Inglês
Notas: | Título em português: Regeneração de plantas a partir de protoplastos de cultivares brasileiros de citros.
Conteúdo: | A procedure is described to regenerate plants from protoplasts of Brazilian citrus cultivars, after isolation, fusion and culture. Protoplasts were isolated from embryogenic cell suspension cultures and from leaf mesophyll of seedlings germinated in vitro. The enzyme solution for protoplast isolation was composed of mannitol (0.7 M), CaCl2 (24.5 mM), NaH2PO4 (0.92 mM), MES (6.15 mM), cellulase (Onozuka RS - Yakult, 1%), macerase (Onozuka R10 - Yakult, 1%) and pectolyase Y-23 (Seishin, 0.2%). Protoplast culture in liquid medium after chemical fusion lead to the formation of callus colonies further adapted to solid medium. Somatic embryo formation occurred spontaneously after two subcultures, on modified MT medium supplemented with 500 mg/L of malt extract. Well defined embryos were germinated in modified MT medium with addition of GA3 (2.0 mM) and malt extract (500 mg/L). Plant regeneration was also achieved by adventitious shoots obtained through direct organogenesis of not well defined embryos in modified MT medium with addition of malt extract (500 mg/L), BAP (1.32 mM), NAA (1.07 mM) and coconut water (10 mL/L). Plantlets were transferred to root medium. Rooted plants were transferred to a greenhouse for further adaptation and development.
Palavras-Chave: | Cultivar improvement; Polietileno glicol.
Thesagro: | Cultura de Tecido; Melhoramento; Planta Porta-Enxerto.
Thesaurus Nal: | Polyethylene glycol; Rootstocks; Tissue culture.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/17268/1/pab98_154.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (17268-1) - UPE - AP - PP - 630.72081 - P474 -  - DIS