Data/hora: 22/06/2024 10:30:43

Biblioteca(s): | Embrapa Amazônia Oriental.
Data corrente: | 06/11/2015
Data da última atualização: | 30/05/2022
Autoria: | SILVEIRA, G. D. da; MOTTA, M. J.; MÜLLER, L. S.; LAMEIRA, O.; ATHAYDE, M. L.; PIANA, M.; ROSA, M. B. da; VIANA, C.; CARVALHO, L. M. de.
Afiliação: | GÉSSICA DOMINGOS DA SILVEIRA, UFSM; MONIQUE JUNG MOTTA, UFSM; LARISSA SABO MÜLLER, UFSM; OSMAR ALVES LAMEIRA, CPATU; MARGARETH L. ATHAYDE, UFSM; MARIANA PIANA, UFSM; MARCELO BARCELLOS DA ROSA, UFSM; CARINE VIANA, UFSM; LEANDRO MACHADO DE CARVALHO, UFSM.
Título: | Determination of phenolic antioxidants in Amazonian medicinal plants by HPLC with pulsed amperometric detection.
Ano de publicação: | 2015
Fonte/Imprenta: | Journal of Liquid Chromatography & Related Technologies, v. 38, n. 13, p. 1259-1266, 2015.
DOI: | 10.1080/10826076.2015.1037450
Idioma: | Inglês
Conteúdo: | A wide range of chromatographic methods for the analysis of phenolic compounds in medicinal plants has been published over the years. However, no chromatographic methods with pulsed amperometric detection using a gold electrode have been described to analyze phenolic acids and flavonoids. Moreover, there is a lack of information regarding a modified mobile phase with &#946;-cyclodextrin to determine these compounds in plants by RP-HPLC. For this reason, the present study developed and validated an HPLC?PAD method to determine 12 phenolic compounds in medicinal plants from Amazonia. The isocratic mobile phase was constituted by sodium phosphate 50 m mol L&#8722;1, methanol 30% (v/v), and &#946;-cyclodextrin 1 m mol L&#8722;1 at pH 2.0. The method demonstrated low detection and quantification limits and robustness. The accuracy ranged from 82?114% for chlorogenic acid and rutin, respectively. Six species of medicinal plants from Amazonia used for medicinal and nutritional purposes were characterized and showed the presence of at least one of the phenolic compounds in the study.
Palavras-Chave: | Compostos fenólicos; Flavonóides.
Thesagro: | Planta Medicinal.
Categoria do assunto: | --
Registro original: | Embrapa Amazônia Oriental (CPATU)
Exemplares: | CPATU - (51745-1) - UPC - AP - DD -  -  -  - DIS