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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
29/08/2002 |
Data da última atualização: |
07/06/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MOTA, F. F. da; NOBREGA, F. F.; MARRIEL, I. E.; PAIVA, E.; SELDIN, L. |
Afiliação: |
IVANILDO EVODIO MARRIEL, CNPMS. |
Título: |
Genetic diversity of Paenibacillus polymyxa populations isolated from the rhizosphere of four cultivars of maize (Zea mays) planted in cerrado soil. |
Ano de publicação: |
2002 |
Fonte/Imprenta: |
Applied Soil Ecology, Amsterdam, v. 20, n. 2, p. 119-132, 2002. |
Idioma: |
Inglês |
Conteúdo: |
A tropical Brazilian soil (Cerrado) was planted with four cultivars of maize (CMS04, CMS11, CMS22 and CMS36) and the genetic diversity of the Paenibacillus polymyxa populations present in their rhizospheres was determined after 90 days of sowing. For that, a total of 67 isolates were identified as R polymyxa by classical biochemical tests and were analyzed for DNA polymorphism with the randomly amplified polymorphic DNA (RAPD) and amplification of repetitive DNA sequences (rep) methods. The amplification patterns obtained using three arbitrary primers and the primer BOXA1R were used separately to construct dendrograms based on the unweighted pair groups method with arithmetic means (UPGMA). Fifty-four genotypic groups were formed when data from different PCR amplification were combined, showing a high level of genetic polymorphism among P. polymyxa strains. A dendrogram based also on combined PCR data, followed by cluster analysis with minimum-variance criteria (Ward) and Euclidean distance, showed that P. polymyxa strains could be divided into two main clusters. One cluster was formed predominantly by strains from maize cultivars CMS04 and CMS36, while the other cluster was formed predominantly by strains of maize cultivars CMS11 and CMS22. Multivariate analysis of variance (MANOVA) allowed the correlation between the genetic structure of P. polymyxa populations and the different cultivars of maize to be studied. The results showed that the strains isolated from the rhizospheres of the different maize cultivars were significantly different. (C) 2002 Elsevier Science B. V. Alls rights reserved. MenosA tropical Brazilian soil (Cerrado) was planted with four cultivars of maize (CMS04, CMS11, CMS22 and CMS36) and the genetic diversity of the Paenibacillus polymyxa populations present in their rhizospheres was determined after 90 days of sowing. For that, a total of 67 isolates were identified as R polymyxa by classical biochemical tests and were analyzed for DNA polymorphism with the randomly amplified polymorphic DNA (RAPD) and amplification of repetitive DNA sequences (rep) methods. The amplification patterns obtained using three arbitrary primers and the primer BOXA1R were used separately to construct dendrograms based on the unweighted pair groups method with arithmetic means (UPGMA). Fifty-four genotypic groups were formed when data from different PCR amplification were combined, showing a high level of genetic polymorphism among P. polymyxa strains. A dendrogram based also on combined PCR data, followed by cluster analysis with minimum-variance criteria (Ward) and Euclidean distance, showed that P. polymyxa strains could be divided into two main clusters. One cluster was formed predominantly by strains from maize cultivars CMS04 and CMS36, while the other cluster was formed predominantly by strains of maize cultivars CMS11 and CMS22. Multivariate analysis of variance (MANOVA) allowed the correlation between the genetic structure of P. polymyxa populations and the different cultivars of maize to be studied. The results showed that the strains isolated from the rhizosp... Mostrar Tudo |
Palavras-Chave: |
Diversidade genetica. |
Thesagro: |
Bactéria; Cerrado; Milho; Rizosfera. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/66483/1/Genetic-diversity-1.pdf
|
Marc: |
LEADER 02330naa a2200229 a 4500 001 1485544 005 2018-06-07 008 2002 bl uuuu u00u1 u #d 100 1 $aMOTA, F. F. da 245 $aGenetic diversity of Paenibacillus polymyxa populations isolated from the rhizosphere of four cultivars of maize (Zea mays) planted in cerrado soil.$h[electronic resource] 260 $c2002 520 $aA tropical Brazilian soil (Cerrado) was planted with four cultivars of maize (CMS04, CMS11, CMS22 and CMS36) and the genetic diversity of the Paenibacillus polymyxa populations present in their rhizospheres was determined after 90 days of sowing. For that, a total of 67 isolates were identified as R polymyxa by classical biochemical tests and were analyzed for DNA polymorphism with the randomly amplified polymorphic DNA (RAPD) and amplification of repetitive DNA sequences (rep) methods. The amplification patterns obtained using three arbitrary primers and the primer BOXA1R were used separately to construct dendrograms based on the unweighted pair groups method with arithmetic means (UPGMA). Fifty-four genotypic groups were formed when data from different PCR amplification were combined, showing a high level of genetic polymorphism among P. polymyxa strains. A dendrogram based also on combined PCR data, followed by cluster analysis with minimum-variance criteria (Ward) and Euclidean distance, showed that P. polymyxa strains could be divided into two main clusters. One cluster was formed predominantly by strains from maize cultivars CMS04 and CMS36, while the other cluster was formed predominantly by strains of maize cultivars CMS11 and CMS22. Multivariate analysis of variance (MANOVA) allowed the correlation between the genetic structure of P. polymyxa populations and the different cultivars of maize to be studied. The results showed that the strains isolated from the rhizospheres of the different maize cultivars were significantly different. (C) 2002 Elsevier Science B. V. Alls rights reserved. 650 $aBactéria 650 $aCerrado 650 $aMilho 650 $aRizosfera 653 $aDiversidade genetica 700 1 $aNOBREGA, F. F. 700 1 $aMARRIEL, I. E. 700 1 $aPAIVA, E. 700 1 $aSELDIN, L. 773 $tApplied Soil Ecology, Amsterdam$gv. 20, n. 2, p. 119-132, 2002.
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Embrapa Milho e Sorgo (CNPMS) |
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3. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | QUEIROGA, V. de P.; GONDIM, T. M. de S.; BORBA, F. G.; ALMEIDA, K. V. de; SOUSA, W. J. B.; QUEIROGAA, D. A. N. Avaliação da qualidade da semente de mamona em função da posição dos racemos na planta e sistemas de beneficiamento. CONGRESSO BRASILEIRO DE MAMONA, 5.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 2.; FÓRUM CAPIXABA DE PINHÃO-MANSO, 1., 2012, Guarapari. Desafios e Oportunidades: anais. Campina Grande: Embrapa Algodão, 2012. p. 412Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Algodão. |
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4. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FIRMINO, P. de T.; SOUZA, W. J. B. de; SILVA, A. C.; ALMEIDA, K. V. de; TEOTÔNIO, M. A.; QUEIROGA, V. de P. Estudo das sementes de gergelim BRS-Seda orgânicas produzidas no sertão piauiense. In: CONGRESSO BRASILEIRO DE MAMONA, 4.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 1., 2010, João Pessoa. Inclusão social e energia: anais. Campina Grande: Embrapa Algodão, 2010.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Algodão. |
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5. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SOUSA, W. J. B. de; ALMEIDA, K. V. de; RIBEIRO FILHO, N. M.; SILVA, F. L. H. da; FIRMINO, P. de T.; ROCHA, A. S. Produção de aguardente de laranja. In: CONGRESSO NORTE-NORDESTE DE QUÍMICA, 3.; ENCONTRO NORTE-NORDESTE DE ENSINO DE QUÍMICA, 1., 2009, São Luís.São Luís, 2009. 1 CD-ROMTipo: Resumo em Anais de Congresso |
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6. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | QUEIROGA, V. de P.; FIRMINO, P. de T.; SILVA, A. C.; BORBA, F. G.; ALMEIDA, K. V. de; SOUSA, W. J. B. de; JERÔNIMO, J. F. Qualidade fisiológica e física das sementes de gergelim de diferentes cores. In: CONGRESSO BRASILEIRO DE MAMONA, 4.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 1., 2010, João Pessoa. Inclusão social e energia: anais. Campina Grande: Embrapa Algodão, 2010.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Algodão. |
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7. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | QUEIROGA, V. de P.; FIRMINO, P. de T.; FREIRE, R. M. M.; SILVA, A. C.; BORBA, F. G.; ALMEIDA, K. V. de; SOUSA, W. J. B. de; JERÔNIMO, J. F. Composição química de sementes de gergelim de diferentes cores. In: CONGRESSO BRASILEIRO DE MAMONA, 4.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 1., 2010, João Pessoa. Inclusão social e energia: anais. Campina Grande: Embrapa Algodão, 2010.Tipo: Artigo em Anais de Congresso |
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8. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | QUEIROGA, V. de P.; FIRMINO, P. de T.; FREIRE, R. M. M.; SILVA, A. C.; BORBA, F. G.; ALMEIDA, K. V. de; SOUSA, W. J. B. de; JERÔNIMO, J. F. Composição mineral de sementes de gergelim de diferentes cores. In: CONGRESSO BRASILEIRO DE MAMONA, 4.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 1., 2010, João Pessoa. Inclusão social e energia: anais. Campina Grande: Embrapa Algodão, 2010.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Algodão. |
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