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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
26/04/2005 |
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Data da última atualização: |
27/02/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SANTOS, B.; MOSCARDI, F. |
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Afiliação: |
CNPSO. |
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Título: |
Improvements in the large scale production of the velvetbean caterpillar, Anticarsia gemmatalis, nucleopolyhedrovirus in the laboratory. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
In: ANNUAL MEETING SOCIETY FOR INVERTEBRATE PATHOLOGY, 36., 2003, Burlington. Program and abstracts... Burlington, 2003. |
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Páginas: |
p. 45. |
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Idioma: |
Inglês |
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Conteúdo: |
The NPV of A. gemmatalis (AgMNPV) is currently being used on over 1,600,000 hectares of soybean in Brazil. Although two private companies had previously attempted to produce the AgMNPV in the laboratory, the ceased production because of the high costs involved, specially those related to artificial diet ingredients (mainly agar and casein), rearing recipients and labor. Thus, large-scale field production of the AgMNPV has been the sole method employed presently, but it is highly dependable on host abundance, wich is affected each year by abiotic and biotic factors, resulting in variable virus yields each season. The objective of this work to improve the laboratory mass production and processing of the AgMNPV, so as to turn the final product cost competitive with available chemical insecticides to control the insect. Initial studies were conducted to substitute the agar and reduce the amount of casein previously utilized for A. gemmatalis rearing and AgMNPV production. The use of Carragena GP-911 compared to the "Invitrogen P.A." and "All Chemistry" agars and reduction of casein by %0% proved successful for virus production, resulting in 86.2 to 95.7% reduction of the diet cost. The modified diet did not alter the survival rate or the weight of caterpillars and pupae. Larvae inoculated as fourth instars with 950,000 OBs/ml of diet in cardboard boxes (30x30) cm and 9 cm high) (350 larvae/box), and maintained at 28o.C provided the highest AgMNPV yield. In this conditions, efficiency of virus production was over 75% (with canibalism being the major cause of yield loss). Laboratory production yielded an average of 58.8 hectare equivalent (HE)/kg of AgMNPV dead larvae, compared to 40 to 50 HE/kg of dead larvae collected under field conditions. Also, the former method resulted in a much lower level of contaminats. The mechanical extratction of the AgMNPV from dead caterpillars (through an adapted fruit juicer) yielded 92.8% of the virus against 75.6% obtained from the manual extraction. Combining the results, the cost of the final AgMNPV product after its production in the laboratory, processing, formulation, quality control, and packaging aws reduced enormously compared to previous procedures, turning the biological insecticide cost competitive with the chemical insecticides. The new laboratory procedures is being proposed to some of the companies producing and commercializing the AgMNPV. MenosThe NPV of A. gemmatalis (AgMNPV) is currently being used on over 1,600,000 hectares of soybean in Brazil. Although two private companies had previously attempted to produce the AgMNPV in the laboratory, the ceased production because of the high costs involved, specially those related to artificial diet ingredients (mainly agar and casein), rearing recipients and labor. Thus, large-scale field production of the AgMNPV has been the sole method employed presently, but it is highly dependable on host abundance, wich is affected each year by abiotic and biotic factors, resulting in variable virus yields each season. The objective of this work to improve the laboratory mass production and processing of the AgMNPV, so as to turn the final product cost competitive with available chemical insecticides to control the insect. Initial studies were conducted to substitute the agar and reduce the amount of casein previously utilized for A. gemmatalis rearing and AgMNPV production. The use of Carragena GP-911 compared to the "Invitrogen P.A." and "All Chemistry" agars and reduction of casein by %0% proved successful for virus production, resulting in 86.2 to 95.7% reduction of the diet cost. The modified diet did not alter the survival rate or the weight of caterpillars and pupae. Larvae inoculated as fourth instars with 950,000 OBs/ml of diet in cardboard boxes (30x30) cm and 9 cm high) (350 larvae/box), and maintained at 28o.C provided the highest AgMNPV yield. In this conditions, effic... Mostrar Tudo |
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Thesagro: |
Baculovirus; Controle Biológico; Praga de Planta; Produção; Soja. |
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Thesaurus Nal: |
Soybeans. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03124nam a2200205 a 4500
001 1467927
005 2020-02-27
008 2003 bl uuuu u00u1 u #d
100 1 $aSANTOS, B.
245 $aImprovements in the large scale production of the velvetbean caterpillar, Anticarsia gemmatalis, nucleopolyhedrovirus in the laboratory.$h[electronic resource]
260 $aIn: ANNUAL MEETING SOCIETY FOR INVERTEBRATE PATHOLOGY, 36., 2003, Burlington. Program and abstracts... Burlington$c2003
300 $ap. 45.
520 $aThe NPV of A. gemmatalis (AgMNPV) is currently being used on over 1,600,000 hectares of soybean in Brazil. Although two private companies had previously attempted to produce the AgMNPV in the laboratory, the ceased production because of the high costs involved, specially those related to artificial diet ingredients (mainly agar and casein), rearing recipients and labor. Thus, large-scale field production of the AgMNPV has been the sole method employed presently, but it is highly dependable on host abundance, wich is affected each year by abiotic and biotic factors, resulting in variable virus yields each season. The objective of this work to improve the laboratory mass production and processing of the AgMNPV, so as to turn the final product cost competitive with available chemical insecticides to control the insect. Initial studies were conducted to substitute the agar and reduce the amount of casein previously utilized for A. gemmatalis rearing and AgMNPV production. The use of Carragena GP-911 compared to the "Invitrogen P.A." and "All Chemistry" agars and reduction of casein by %0% proved successful for virus production, resulting in 86.2 to 95.7% reduction of the diet cost. The modified diet did not alter the survival rate or the weight of caterpillars and pupae. Larvae inoculated as fourth instars with 950,000 OBs/ml of diet in cardboard boxes (30x30) cm and 9 cm high) (350 larvae/box), and maintained at 28o.C provided the highest AgMNPV yield. In this conditions, efficiency of virus production was over 75% (with canibalism being the major cause of yield loss). Laboratory production yielded an average of 58.8 hectare equivalent (HE)/kg of AgMNPV dead larvae, compared to 40 to 50 HE/kg of dead larvae collected under field conditions. Also, the former method resulted in a much lower level of contaminats. The mechanical extratction of the AgMNPV from dead caterpillars (through an adapted fruit juicer) yielded 92.8% of the virus against 75.6% obtained from the manual extraction. Combining the results, the cost of the final AgMNPV product after its production in the laboratory, processing, formulation, quality control, and packaging aws reduced enormously compared to previous procedures, turning the biological insecticide cost competitive with the chemical insecticides. The new laboratory procedures is being proposed to some of the companies producing and commercializing the AgMNPV.
650 $aSoybeans
650 $aBaculovirus
650 $aControle Biológico
650 $aPraga de Planta
650 $aProdução
650 $aSoja
700 1 $aMOSCARDI, F.
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Registro original: |
Embrapa Soja (CNPSO) |
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Biblioteca |
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| Registros recuperados : 64 | |
| 4. |  | TAKEDA, H. H.; FIRMINO, A.; MATTOSO, L. H. C. Língua eletrônica para identificação de paladares doce, salgado, azedo e amargo. In: SIMPÓSIO EM CIÊNCIA E ENGENHARIA DE MATERIAIS, SICEM, 6, out. 2003, São Carlos. Resumos extendidos... São Carlos : USP, 2003. p.27-28.| Biblioteca(s): Embrapa Instrumentação. |
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| 7. | | CHAGAS, H. A.; FIRMINO, A. C.; SILVA, R. B.; FURTADO, E. L.; ZANOTTO, M. D. Caracterização molecular de isolados de AMPHOBOTRYS RICINI, da mamoneira no Brasil. In: CONGRESSO BRASILEIRO DE MAMONA, 5.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 2.; FÓRUM CAPIXABA DE PINHÃO-MANSO, 1., 2012, Guarapari. Desafios e Oportunidades: anais. Campina Grande: Embrapa Algodão, 2012. p. 201| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
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| 12. | | TAKEDA, H. H.; FIRMINO, A.; RIUL JÚNIOR, A.; FONSECA, F. J.; MATTOSO, L. H. C. Língua eletrônica para diferenciação de paladares doce, salgado, azedo e amargo. In: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS, 5. nov. 2003, Campinas, SP. [Resumos...] Campinas: UNICAMP, 2003. [CD-ROM].| Biblioteca(s): Embrapa Instrumentação. |
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| 13. | | NAKASU, E. Y. T.; FIRMINO, A. A. P.; DIAS, S. C.; GROSSI-de-SÁ, M. F. Identificação de receptores do bicudo do algodoeiro (Anthonomus grandis) para toxinas cry. In : ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 12., 2007, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. p. 71.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | FIRMINO, A.; BORATO, C. E.; LEITE, F. L.; RIUL JR.; A.; BERNARDES FILHO, R.; MATTOSO, L. H. C. AFM as a tool for development sensors. Acta Microscopica, Maracaibo, v. 12, Apr. 2003. Supplement A. CD de trabalhos e resumos apresentados no Second Latin American Symposium on Scanning Probe Microscopy, Búzios, Apr. 2003. 2 f. Invited paper. 1 CD-ROM.| Biblioteca(s): Embrapa Instrumentação. |
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| 15. | | WILCKEN, C.; BARBOSA, L. R.; ZACHE, B.; FIRMINO, A.; SA, L. A. N. de; ZANUNCIO, J.; JUNQUEIRA, L. Biological control of the bronze bug, Thaumastocoris peregrinus, in eucalyptus plantations in Brazil. The International Forestry Review, v. 16, n. 5, p. 325, 2014. Edição dos abstracts do 24º IUFRO World Congress, 2014, Salt Lake City. Sustaining forests, sustaining people: the role of research.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 16. | | BORATO, C. E.; FIRMINO, A.; ALMEIDA, T. A. S.; RIUL JÚNIOR, A.; FONSECA, F. J.; MATTOSO, L. H. C. Uso de polímeros condutores em língua eletrônica para a análise de vinhos e cafés. In: CONGRESSO BRASILEIRO DE POLÍMEROS. 7., nov. 2003., Belo Horizonte, MG. Anais... São Carlos: Associação Brasileira de Polímeros, 2003. p. 421-422.| Biblioteca(s): Embrapa Instrumentação. |
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| 17. | | NAKASU, E. Y. T; FIRMINO, A. A. P.; DIAS, S. C.; OLIVEIRA, G. R.; GROSSI DE SÁ, M. F. Estratégias para identificação e caracterização de receptores do bicudo do algodoeiro (Anthonomus grandis) para toxinas Cry. In: WORKSHOP INTERAÇÃO MOLECULAR PLANTA-PRAGAS, 2., 2007, Brasília, DF. II Workshop Interação Molecular Planta-Praga. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. p. 66-70. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 229).| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 18. | | PATERNO, L. G.; CONSOLIN FILHO, N.; VENÂNCIO, E. C.; BORATO, C. E.; FIRMINO, A.; MATTOSO, L. H. C. Fabrication of ultrathin films of conducting polymers by the self-assembly technique. In: REUNIÃO DA REDE DE NANOBIOTECNOLOGIA - Rede Nanobiotec, 2., 2003, Campinas. Livro de Resumos... Campinas: UNICAMP, 2003. v. 2. p. Seminário de Encerramento das Atividades da Primeira Fase da Rede de Nanobiotecnologia. p. 39-40.| Biblioteca(s): Embrapa Instrumentação. |
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| 19. | | VENANCIO, E. C.; PATERNO, L. G.; CONSOLIN FILHO, N.; BORATO, C. E.; FIRMINO, A.; MATTOSO, L. H. Influence of the pH and substrate immersion time on the adsorption of Poly(o-ethoxyaniline) in self-assembled films. Journal of the Brazilian Chemical Society, São Paulo, v. 16, n. 3B, p. 558-564, 2005.| Biblioteca(s): Embrapa Instrumentação. |
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| Registros recuperados : 64 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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