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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
01/07/2004 |
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Data da última atualização: |
26/07/2017 |
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Autoria: |
PINEDO, F. J. R.; MOSCARDI, F.; LUQUE, T.; OLSZEWSKI, J. A.; RIBEIRO, B. M. |
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Título: |
Inactivation of the ecdysteroid UDP-glucosyltransferase (egt) gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) improves its virulence towards its insect host. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Biological Control, v. 27, n. 3, p. 336-344, July 2003. |
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Idioma: |
Inglês |
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Conteúdo: |
Some baculovirus have been genetically modified for the inactivation of their ecdysteroid
glucosyltransferase (egt) gene, and these viruses were shown to kill infected larvae more rapidly when compared to wild-type virus infections. We have previously identified, cloned, and sequenced the egt gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV). Here we present data regarding the construction of an egt minus (egt?x2212;) AgMNPV and its virulence towards its insect host. We have inserted an hsp70-lacZ (3.7 kb) gene cassette into the egt gene open reading frame (ORF) and purified a recombinant AgMNPV (vAgEGT?x394;-lacZ). Bioassays with third-instar A. gemmatalis larvae showed that viral occlusion body (OB) production were consistently lower from infections with vAgEGT?x394;-lacZ compared to the wild-type virus. A mean of 20.4Ã?108 OBs/g/larva and 40.7Ã?108 OBs/g/larva was produced from vAgEGT?x394;-lacZ and AgMNPV infections, respectively. The mean lethal concentration which killed 50% of insects in a treatment group (LC50) for the 10th day after virus treatment (DAT) was 3.9-fold higher for the wild-type virus compared to vAgEGT?x394;-lacZ. The recombinant virus killed A. gemmatalis larvae significantly faster (ca. 1?x2013;2.8 days), than the wild-type
AgMNPV. Therefore, the vAgEGT?x394;-lacZ was more efficacious for the control of A.
gemmatalis larvae (in bioassays) compared to wild-type AgMNPV. |
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Palavras-Chave: |
Ecdysteroid glucosyltransferase; Velvetbean caterpillar. |
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Thesagro: |
Anticarsia Gemmatalis; Baculovirus; Controle Biológico; Engenharia Genética. |
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Categoria do assunto: |
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Marc: |
LEADER 02224naa a2200241 a 4500
001 1467192
005 2017-07-26
008 2003 bl --- 0-- u #d
100 1 $aPINEDO, F. J. R.
245 $aInactivation of the ecdysteroid UDP-glucosyltransferase (egt) gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) improves its virulence towards its insect host.
260 $c2003
520 $aSome baculovirus have been genetically modified for the inactivation of their ecdysteroid glucosyltransferase (egt) gene, and these viruses were shown to kill infected larvae more rapidly when compared to wild-type virus infections. We have previously identified, cloned, and sequenced the egt gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV). Here we present data regarding the construction of an egt minus (egt?x2212;) AgMNPV and its virulence towards its insect host. We have inserted an hsp70-lacZ (3.7 kb) gene cassette into the egt gene open reading frame (ORF) and purified a recombinant AgMNPV (vAgEGT?x394;-lacZ). Bioassays with third-instar A. gemmatalis larvae showed that viral occlusion body (OB) production were consistently lower from infections with vAgEGT?x394;-lacZ compared to the wild-type virus. A mean of 20.4Ã?108 OBs/g/larva and 40.7Ã?108 OBs/g/larva was produced from vAgEGT?x394;-lacZ and AgMNPV infections, respectively. The mean lethal concentration which killed 50% of insects in a treatment group (LC50) for the 10th day after virus treatment (DAT) was 3.9-fold higher for the wild-type virus compared to vAgEGT?x394;-lacZ. The recombinant virus killed A. gemmatalis larvae significantly faster (ca. 1?x2013;2.8 days), than the wild-type AgMNPV. Therefore, the vAgEGT?x394;-lacZ was more efficacious for the control of A. gemmatalis larvae (in bioassays) compared to wild-type AgMNPV.
650 $aAnticarsia Gemmatalis
650 $aBaculovirus
650 $aControle Biológico
650 $aEngenharia Genética
653 $aEcdysteroid glucosyltransferase
653 $aVelvetbean caterpillar
700 1 $aMOSCARDI, F.
700 1 $aLUQUE, T.
700 1 $aOLSZEWSKI, J. A.
700 1 $aRIBEIRO, B. M.
773 $tBiological Control$gv. 27, n. 3, p. 336-344, July 2003.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 544 | |
| 363. |  | MATSUBARA, F. H.; MARQUES, C. R. G.; LEVY, S. M.; MOSCARDI, F.; FALLEIROS, A. M. F. Estudo dos hemócitos de larvas de Anticarsia gemmtalis (Hübner, 1818) (Lepidoptera: Noctuidae) suscetíveis e resistentes ao AgMNPV à microscopia eletrônica de varredura. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação: anais. Viçosa, MG: UFV, 2008. 1 CD-ROM. Resumo 1314-2.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 364. | | CUNHA, F.; GOMEZ, D. R. S.; SILVA, J. J. da; ALEXANDRE, T. M.; MOSCARDI, F. Genetic diversity of the sunflower caterpillar (Chlosyne lacinia saundersii Doubleday and Hewitson) ( Lepidoptera: Nymphalidae) populations determined by molecular RAPD markers. Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 82, n. 4, p. 1127-1136, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja. |
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| 368. | | MOSCARDI, F.; KASTELIC, J. G.; POLLATO, S. L. B.; CORREA-FERREIRA, B.S. Epizootioloy of Anticarsia gemmatalis nuclear polyhedrosis virus in the soybean agroecosystem. In: ANNUAL MEETING SOCIETY FOR INVERTEBRATE PATHOLOGY, 20., 1987, Gainesville, Florida. Abstracts... Gainesville, SIP, 1987. p.96-97.| Biblioteca(s): Embrapa Soja. |
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| 370. | | CUNHA, F.; MOSCARDI, F.; ALEXANDRE, T. M.; SOSA-GOMEZ, D. R.; NEGRÃO, I. L. Flutuação populacional da lagarta-preta-do-girassol Chlosyne lacinia Saundersii (Lepidoptera: Nymphalidae) em plantas de girassol em Londrina PR. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação: anais. Viçosa, MG: UFV, 2008. 1 CD-ROM. Resumo 2274-1.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 371. | | MORALES, L.; MOSCARDI, F.; SÓSA-GOMEZ, D. R.; PARO, F. E.; SOLDORIO, I. L. Fluorescent brighteners improve Anticarsia gemmatalis (Lepidoptera: Noctuidae) nucleopolyhedrovirus (AgMNPV) activity on AgMNPV-susceptible and resistant strains of the insect. Biological Control, Orlando, v. 20, n. 3, p. 247-253, Mar. 2001.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - B |
| Biblioteca(s): Embrapa Soja. |
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| 376. | | HOFFMANN-CAMPO, C. B.; OLIVEIRA, L. J.; MOSCARDI, F.; CORRÊA-FERREIRA, B. S.; CORSO, I. C. Pragas que atacam plântulas, hastes e pecíolos da soja. In: HOFFMANN-CAMPO, C. B.; CORRÊA-FERREIRA, B. S.; MOSCARDI, F. Soja: manejo integrado de insetos e outros Artrópodes-praga. Brasília, DF: Embrapa, 2012. p. 145-212.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Soja. |
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| 378. | | CUNHA, F.; MOSCARDI, F.; ALEXANDRE, T. M.; SOSA-GOMEZ, D. R. Preferência para oviposição e ocorrência da lagarta-preta-do-girassol (Chlosyne lacinia saundersii) em diferentes plantas hospedeiras com e sem a presença do girassol. In: REUNIÃO NACIONAL DE PESQUISA DE GIRASSOL, 17.; SIMPÓSIO NACIONAL SOBRE A CULTURA DO GIRASSOL, 5., 2007, Uberaba. Anais... Londrina: Embrapa Soja, 2007. p. 17-20. (Embrapa Soja. Documentos, 292). Organizado por Odilon Ferreira Saraiva, Regina Maria Villas Bôas de Campos Leite, Simone Ery Grosskopf.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 544 | |
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