Data/hora: 25/06/2024 13:56:44

Biblioteca(s): 	Embrapa Soja.	
Data corrente: 	01/07/2004	
Data da última atualização: 	26/07/2017	
Autoria: 	PINEDO, F. J. R.; MOSCARDI, F.; LUQUE, T.; OLSZEWSKI, J. A.; RIBEIRO, B. M.	
Título: 	Inactivation of the ecdysteroid UDP-glucosyltransferase (egt) gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) improves its virulence towards its insect host.	
Ano de publicação: 	2003	
Fonte/Imprenta: 	Biological Control, v. 27, n. 3, p. 336-344, July 2003.	
Idioma: 	Inglês	
Conteúdo: 	Some baculovirus have been genetically modified for the inactivation of their ecdysteroid
glucosyltransferase (egt) gene, and these viruses were shown to kill infected larvae more rapidly when compared to wild-type virus infections. We have previously identified, cloned, and sequenced the egt gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV). Here we present data regarding the construction of an egt minus (egt?x2212;) AgMNPV and its virulence towards its insect host. We have inserted an hsp70-lacZ (3.7 kb) gene cassette into the egt gene open reading frame (ORF) and purified a recombinant AgMNPV (vAgEGT?x394;-lacZ). Bioassays with third-instar A. gemmatalis larvae showed that viral occlusion body (OB) production were consistently lower from infections with vAgEGT?x394;-lacZ compared to the wild-type virus. A mean of 20.4Ã?108 OBs/g/larva and 40.7Ã?108 OBs/g/larva was produced from vAgEGT?x394;-lacZ and AgMNPV infections, respectively. The mean lethal concentration which killed 50% of insects in a treatment group (LC50) for the 10th day after virus treatment (DAT) was 3.9-fold higher for the wild-type virus compared to vAgEGT?x394;-lacZ. The recombinant virus  killed A. gemmatalis larvae significantly faster (ca. 1?x2013;2.8 days), than the wild-type
AgMNPV. Therefore, the vAgEGT?x394;-lacZ was more efficacious for the control of A.
gemmatalis larvae (in bioassays) compared to wild-type AgMNPV.	
Palavras-Chave: 	Ecdysteroid glucosyltransferase; Velvetbean caterpillar.	
Thesagro: 	Anticarsia Gemmatalis; Baculovirus; Controle Biológico; Engenharia Genética.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Soja (CNPSO)	
Exemplares: 	CNPSO - (24129-1) - UPC - SP - -- - 10065 -  - 10065 - DIS