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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
04/05/1999 |
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Data da última atualização: |
04/05/1999 |
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Autoria: |
SARTORE, G.; STORMONT, C.; MORRIS, B. G.; GRUNDER, A. A. |
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Título: |
Multiple electrophoretic forms of carbonic anhydrase in red cells of domestic cattle (Bos taurus) and American buffalo (Bison bison). |
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Ano de publicação: |
1969 |
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Fonte/Imprenta: |
Genetics, v.61, p.823-831, Apr. 1969. |
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Idioma: |
Inglês |
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Conteúdo: |
By conbining the methodology of starch gel electrophoresis (Smithies 1955) with histochemical staining, it is possible, as shown by Hunter and Markert (1957), to demonstrate numerous zones of enzymatic activity directly in the supporting medium. Markert and Moller (1959) introduced the term "isozyme" which they used to denote multiple molecular forms of the same enzume, a cogent example being the multiple molecular forms of lactate dehydrogenase. Using this approach, which has been referred to as the "zymogram display", numerous isozyme polymorphisms have since been reported as reviewed by Shaw (1965). Among such reports was one from this laboratory (Grunder, sartore, and Stormont 1965) concerned with the description system of aliesterases demonstrable in hemolysates of rabbit red cells and apparently under the control of a pair of codominant autosomal alleles. Somewhat analogous systems of red cell esterases have also been described in studies of mice (Pelzer 1965), Peromyscus (Randerson 1965), and primates (Tashian 1965). Concomitantly with our studies of rabbit red cell esterases we sought to detect electroporetic variation in the red cell esterases of domestic cattle and American buffalo, and were successful. However, in line with certain observations made by Tashian and colleagues (see Tashian 1965), we found that these particular isozymes are more appropriately classified as carbonic anhydrases (CA) even though they do exhibit esterase activity. The results of those studies are the subject of this report. MenosBy conbining the methodology of starch gel electrophoresis (Smithies 1955) with histochemical staining, it is possible, as shown by Hunter and Markert (1957), to demonstrate numerous zones of enzymatic activity directly in the supporting medium. Markert and Moller (1959) introduced the term "isozyme" which they used to denote multiple molecular forms of the same enzume, a cogent example being the multiple molecular forms of lactate dehydrogenase. Using this approach, which has been referred to as the "zymogram display", numerous isozyme polymorphisms have since been reported as reviewed by Shaw (1965). Among such reports was one from this laboratory (Grunder, sartore, and Stormont 1965) concerned with the description system of aliesterases demonstrable in hemolysates of rabbit red cells and apparently under the control of a pair of codominant autosomal alleles. Somewhat analogous systems of red cell esterases have also been described in studies of mice (Pelzer 1965), Peromyscus (Randerson 1965), and primates (Tashian 1965). Concomitantly with our studies of rabbit red cell esterases we sought to detect electroporetic variation in the red cell esterases of domestic cattle and American buffalo, and were successful. However, in line with certain observations made by Tashian and colleagues (see Tashian 1965), we found that these particular isozymes are more appropriately classified as carbonic anhydrases (CA) even though they do exhibit esterase activity. The results of those st... Mostrar Tudo |
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Palavras-Chave: |
American buffalo; Bufalo americano. |
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Thesagro: |
Bos Taurus; Gado; Genética. |
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Thesaurus Nal: |
Bison bison; cattle; genetics. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02234naa a2200253 a 4500
001 1795570
005 1999-05-04
008 1969 bl --- 0-- u #d
100 1 $aSARTORE, G.
245 $aMultiple electrophoretic forms of carbonic anhydrase in red cells of domestic cattle (Bos taurus) and American buffalo (Bison bison).
260 $c1969
520 $aBy conbining the methodology of starch gel electrophoresis (Smithies 1955) with histochemical staining, it is possible, as shown by Hunter and Markert (1957), to demonstrate numerous zones of enzymatic activity directly in the supporting medium. Markert and Moller (1959) introduced the term "isozyme" which they used to denote multiple molecular forms of the same enzume, a cogent example being the multiple molecular forms of lactate dehydrogenase. Using this approach, which has been referred to as the "zymogram display", numerous isozyme polymorphisms have since been reported as reviewed by Shaw (1965). Among such reports was one from this laboratory (Grunder, sartore, and Stormont 1965) concerned with the description system of aliesterases demonstrable in hemolysates of rabbit red cells and apparently under the control of a pair of codominant autosomal alleles. Somewhat analogous systems of red cell esterases have also been described in studies of mice (Pelzer 1965), Peromyscus (Randerson 1965), and primates (Tashian 1965). Concomitantly with our studies of rabbit red cell esterases we sought to detect electroporetic variation in the red cell esterases of domestic cattle and American buffalo, and were successful. However, in line with certain observations made by Tashian and colleagues (see Tashian 1965), we found that these particular isozymes are more appropriately classified as carbonic anhydrases (CA) even though they do exhibit esterase activity. The results of those studies are the subject of this report.
650 $aBison bison
650 $acattle
650 $agenetics
650 $aBos Taurus
650 $aGado
650 $aGenética
653 $aAmerican buffalo
653 $aBufalo americano
700 1 $aSTORMONT, C.
700 1 $aMORRIS, B. G.
700 1 $aGRUNDER, A. A.
773 $tGenetics$gv.61, p.823-831, Apr. 1969.
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Registro original: |
Embrapa Pantanal (CPAP) |
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