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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
19/07/2017 |
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Data da última atualização: |
19/07/2017 |
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Autoria: |
KANAI, Y.; YAMASAKI, J.; TAKEUCHI, Y.; KOIKE, H.; FUJIYAMA, M.; MORI, Y. |
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Título: |
Electrophysiological recording of hypothalamic gnrh pulse generator activity in castrated male goats. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Journal of Reproduction and Development, v. 41, n. 1, p. 57-62, Fev. 1995. |
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DOI: |
http://doi.org/10.1262/jrd.41.57 |
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Idioma: |
Inglês |
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Conteúdo: |
Electrophysiological recording of the hypothalamic GnRH pulse generator activity was attempted in 3 long-term castrated male goats in which electrodes had been implanted in the medial basal hypothalamus (MBH). While multiple unit activity (MUA) signals from the electrode were continuously recorded, each animal received subcutaneous implants containing testosterone for 7 days (Experiment 1). They were similarly treated with estradiol alone, estradiol plus testosterone, and testosterone alone for 7 days each consecutively (Experiment 2). These steroidal treatments enabled to maintain the plasma levels of testosterone and estradiol within physiological ranges at 2-4 ng/ml and 3-5 pg/ml, respectively. Blood samples for the determination of pulsatile LH secretion were collected at 6-min intervals for 4 h on the last day of each treatment period. The short-lived sudden increase in MUA spikes (MUA volley) was detected being always preceding the occurrence of the LH pulse in the peripheral blood, and this synchrony of MUA volley with the LH pulse was constant in all the animals regardless of steroidal treatments. Testosterone treatment resulted in the increase of the MUA volley intervals from 27 min to 43 min (Experiment 1). Treatment with estradiol in Experiment 2 also resulted in prolonged MUA volley intervals similar to those of testosterone treatment and there was no additive or synergistic effect between these two steroids. The present study demonstrated that the electrophysiological manifestation of the hypothalamic GnRH pulse generator could be successfully monitored in male goats as reported in females, and it is suggested that estradiol may exert a negative feedback effect on the GnRH pulse generator activity in a similar manner to that of testosterone but at much lower doses in male goats. MenosElectrophysiological recording of the hypothalamic GnRH pulse generator activity was attempted in 3 long-term castrated male goats in which electrodes had been implanted in the medial basal hypothalamus (MBH). While multiple unit activity (MUA) signals from the electrode were continuously recorded, each animal received subcutaneous implants containing testosterone for 7 days (Experiment 1). They were similarly treated with estradiol alone, estradiol plus testosterone, and testosterone alone for 7 days each consecutively (Experiment 2). These steroidal treatments enabled to maintain the plasma levels of testosterone and estradiol within physiological ranges at 2-4 ng/ml and 3-5 pg/ml, respectively. Blood samples for the determination of pulsatile LH secretion were collected at 6-min intervals for 4 h on the last day of each treatment period. The short-lived sudden increase in MUA spikes (MUA volley) was detected being always preceding the occurrence of the LH pulse in the peripheral blood, and this synchrony of MUA volley with the LH pulse was constant in all the animals regardless of steroidal treatments. Testosterone treatment resulted in the increase of the MUA volley intervals from 27 min to 43 min (Experiment 1). Treatment with estradiol in Experiment 2 also resulted in prolonged MUA volley intervals similar to those of testosterone treatment and there was no additive or synergistic effect between these two steroids. The present study demonstrated that the electrophysiol... Mostrar Tudo |
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Palavras-Chave: |
GnRH; GnRH pulse generator; Gonadal steroids; LH; MUA volley. |
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Thesaurus Nal: |
Goats. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02568naa a2200265 a 4500
001 2072873
005 2017-07-19
008 1995 bl uuuu u00u1 u #d
024 7 $ahttp://doi.org/10.1262/jrd.41.57$2DOI
100 1 $aKANAI, Y.
245 $aElectrophysiological recording of hypothalamic gnrh pulse generator activity in castrated male goats.
260 $c1995
520 $aElectrophysiological recording of the hypothalamic GnRH pulse generator activity was attempted in 3 long-term castrated male goats in which electrodes had been implanted in the medial basal hypothalamus (MBH). While multiple unit activity (MUA) signals from the electrode were continuously recorded, each animal received subcutaneous implants containing testosterone for 7 days (Experiment 1). They were similarly treated with estradiol alone, estradiol plus testosterone, and testosterone alone for 7 days each consecutively (Experiment 2). These steroidal treatments enabled to maintain the plasma levels of testosterone and estradiol within physiological ranges at 2-4 ng/ml and 3-5 pg/ml, respectively. Blood samples for the determination of pulsatile LH secretion were collected at 6-min intervals for 4 h on the last day of each treatment period. The short-lived sudden increase in MUA spikes (MUA volley) was detected being always preceding the occurrence of the LH pulse in the peripheral blood, and this synchrony of MUA volley with the LH pulse was constant in all the animals regardless of steroidal treatments. Testosterone treatment resulted in the increase of the MUA volley intervals from 27 min to 43 min (Experiment 1). Treatment with estradiol in Experiment 2 also resulted in prolonged MUA volley intervals similar to those of testosterone treatment and there was no additive or synergistic effect between these two steroids. The present study demonstrated that the electrophysiological manifestation of the hypothalamic GnRH pulse generator could be successfully monitored in male goats as reported in females, and it is suggested that estradiol may exert a negative feedback effect on the GnRH pulse generator activity in a similar manner to that of testosterone but at much lower doses in male goats.
650 $aGoats
653 $aGnRH
653 $aGnRH pulse generator
653 $aGonadal steroids
653 $aLH
653 $aMUA volley
700 1 $aYAMASAKI, J.
700 1 $aTAKEUCHI, Y.
700 1 $aKOIKE, H.
700 1 $aFUJIYAMA, M.
700 1 $aMORI, Y.
773 $tJournal of Reproduction and Development$gv. 41, n. 1, p. 57-62, Fev. 1995.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
29/04/2010 |
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Data da última atualização: |
06/05/2010 |
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Tipo da produção científica: |
Boletim de Pesquisa e Desenvolvimento |
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Autoria: |
BARRETO, A. C.; FERNANDES, M. F. |
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Afiliação: |
ANTONIO CARLOS BARRETO, CPATC; MARCELO FERREIRA FERNANDES, CPATC. |
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Título: |
Manejo da adubação verde sobre atributos químicos de um argissolo amarelo dos tabuleiros costeiros. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Aracaju: Embrapa Tabuleiros Coteiros, 2009. |
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Páginas: |
23 p. |
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Série: |
(Embrapa Tabuleiros Costeiros. Boletim de Pesquisa e Desenvolvimento, 55). |
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Idioma: |
Português |
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Palavras-Chave: |
Argissolo; Consórcio de planta. |
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Thesagro: |
Adubo Verde; Solo. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPATC-2010/21514/1/bp-55.pdf
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Marc: |
LEADER 00612nam a2200181 a 4500
001 1748249
005 2010-05-06
008 2009 bl uuuu u0uu1 u #d
100 1 $aBARRETO, A. C.
245 $aManejo da adubação verde sobre atributos químicos de um argissolo amarelo dos tabuleiros costeiros.$h[electronic resource]
260 $aAracaju: Embrapa Tabuleiros Coteiros$c2009
300 $a23 p.
490 $a(Embrapa Tabuleiros Costeiros. Boletim de Pesquisa e Desenvolvimento, 55).
650 $aAdubo Verde
650 $aSolo
653 $aArgissolo
653 $aConsórcio de planta
700 1 $aFERNANDES, M. F.
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Registro original: |
Embrapa Tabuleiros Costeiros (CPATC) |
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