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Registro Completo |
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Biblioteca(s): |
Embrapa Agropecuária Oeste; Embrapa Cerrados; Embrapa Soja; Embrapa Unidades Centrais. |
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Data corrente: |
28/10/2004 |
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Data da última atualização: |
28/10/2009 |
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Autoria: |
SHARMA, R. D.; MOREIRA, W. A.; ALVES, R. T. |
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Título: |
Eficiência de endotoxinas de Bacillus spp. no controle de heterodera glycines ichinohe na soja. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Planaltina, DF: Embrapa Cerrados, 2003. |
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Páginas: |
15 p. |
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Série: |
(Embrapa Cerrados. Boletim de Pesquisa e Desenvolvimento, 78). |
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Idioma: |
Português |
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Conteúdo: |
RESUMO: Avaliou-se a eficiência de endotoxinas de Bacillus sphaericus (Bs 2362), B. thuringiensis var. Israelensis (Bti-H-14) e B. thuringiensis var. Kurstaki (Btk-Hd-1) no controle de nematóide de cisto Heterodera glycines, na soja cv. Cristalina, em casa de vegetação. Cinco doses (0, 250, 500, 1000, e 1500 mg) de cada toxina, com 108 esporos por grama, foram comparadas com 150 mg de aldicarb em um experimento com cinco repetições. Os produtos foram incorporados a 2 cm de profundidade e o solo irrigado com 100 mL de água destilada. Uma testemunha, com solo auto-clavado, foi incluído, para comparação do crescimento das plantas. Dez dias depois da aplicação de endotoxinas e aldicarb, uma plântula de soja, depois da emergência, foi transplantada em cada vaso contendo 1 kg de solo. Passados oitenta e três dias, avaliou-se peso de grãos/planta para diferentes doses de toxinas. Os tratamentos com endotoxina Btk-HD-1 na dosagem de 1500 mg/kg de solo e solo autoclavado diferiram significativamente (P<0.05) da dose zero. Os pesos dos grãos nos tratamentos com a endotoxina Btk-HD-1 dosagem de 1500 mg/kg de solo e solo autoclavado foram respectivamente 61 e 85 % maiores. Nenhuma das endotoxinas usadas neste estudo reduziu significativamente (P<0,05) a população final de nematóides em relação o controle. ABSTRACT: In a greenhouse pot experiment, efficiency of endotoxins obtained from pure cultures of Bacillus sphaericus (Bs 2362), B. thuringiensis var. israelensis (Bti-H-14) and B. thuringiensis var. kurstaki (Btk-Hd-1) was evaluated against soybean cyst nematode, Heterodera glycines on soybean cv. Cristalina. Five doses (0, 250, 500, 1000 and 1500 mg) of each toxin with 1.108 spores per gram and 150 mg aldicarb were incorporated in upper 2-cm soil layer followed by application of 100 mL distilled water/kg of soil. Autoclaved soil was used as control for comparing plant growth with treatments. The experimental design was a completely randomized, with five replicates. Ten days after the application of endotoxins, a three-day after emergence soybean seedling was transplanted in each pot. Eighty-three days later, the increase in grain weight/plant and final populations of soybean cyst nematodes were evaluated. In general, grain production was very low. Grain weights for treatments with endotoxin Btk-HD-1 dose 1500 mg/Kg of soil and autoclaved soil differed significantly (P<0.05) from the control, yielding respectively 61% and 85 % higher than the control. None of the endotoxins used in this study could significantly (P<0.05) reduce the final nematode populations in relation to the untreated control (autoclaved soil). MenosRESUMO: Avaliou-se a eficiência de endotoxinas de Bacillus sphaericus (Bs 2362), B. thuringiensis var. Israelensis (Bti-H-14) e B. thuringiensis var. Kurstaki (Btk-Hd-1) no controle de nematóide de cisto Heterodera glycines, na soja cv. Cristalina, em casa de vegetação. Cinco doses (0, 250, 500, 1000, e 1500 mg) de cada toxina, com 108 esporos por grama, foram comparadas com 150 mg de aldicarb em um experimento com cinco repetições. Os produtos foram incorporados a 2 cm de profundidade e o solo irrigado com 100 mL de água destilada. Uma testemunha, com solo auto-clavado, foi incluído, para comparação do crescimento das plantas. Dez dias depois da aplicação de endotoxinas e aldicarb, uma plântula de soja, depois da emergência, foi transplantada em cada vaso contendo 1 kg de solo. Passados oitenta e três dias, avaliou-se peso de grãos/planta para diferentes doses de toxinas. Os tratamentos com endotoxina Btk-HD-1 na dosagem de 1500 mg/kg de solo e solo autoclavado diferiram significativamente (P<0.05) da dose zero. Os pesos dos grãos nos tratamentos com a endotoxina Btk-HD-1 dosagem de 1500 mg/kg de solo e solo autoclavado foram respectivamente 61 e 85 % maiores. Nenhuma das endotoxinas usadas neste estudo reduziu significativamente (P<0,05) a população final de nematóides em relação o controle. ABSTRACT: In a greenhouse pot experiment, efficiency of endotoxins obtained from pure cultures of Bacillus sphaericus (Bs 2362), B. thuringiensis var. israelensis (Bti-H-14) and B. thu... Mostrar Tudo |
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Palavras-Chave: |
Bacterial endotoxins; Biocontrol; Israelensis; Kurstaki; Nematóide do cisto; Nematóide-do-cisto; Nematóide-do-cisto da soja; Soybean cyst nematode; thuringiensis var. |
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Thesagro: |
Bactéria; Cisto; Controle Biológico; Heterodera Glycines; Nematóide; Soja. |
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Thesaurus Nal: |
Bacillus sphaericus; biological control; Nematoda; soybeans. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPAC-2009/24969/1/bolpd_78.pdf
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Marc: |
LEADER 03772nam a2200385 a 4500
001 1567955
005 2009-10-28
008 2003 bl uuuu u0uu1 u #d
100 1 $aSHARMA, R. D.
245 $aEficiência de endotoxinas de Bacillus spp. no controle de heterodera glycines ichinohe na soja.
260 $aPlanaltina, DF: Embrapa Cerrados$c2003
300 $a15 p.
490 $a(Embrapa Cerrados. Boletim de Pesquisa e Desenvolvimento, 78).
520 $aRESUMO: Avaliou-se a eficiência de endotoxinas de Bacillus sphaericus (Bs 2362), B. thuringiensis var. Israelensis (Bti-H-14) e B. thuringiensis var. Kurstaki (Btk-Hd-1) no controle de nematóide de cisto Heterodera glycines, na soja cv. Cristalina, em casa de vegetação. Cinco doses (0, 250, 500, 1000, e 1500 mg) de cada toxina, com 108 esporos por grama, foram comparadas com 150 mg de aldicarb em um experimento com cinco repetições. Os produtos foram incorporados a 2 cm de profundidade e o solo irrigado com 100 mL de água destilada. Uma testemunha, com solo auto-clavado, foi incluído, para comparação do crescimento das plantas. Dez dias depois da aplicação de endotoxinas e aldicarb, uma plântula de soja, depois da emergência, foi transplantada em cada vaso contendo 1 kg de solo. Passados oitenta e três dias, avaliou-se peso de grãos/planta para diferentes doses de toxinas. Os tratamentos com endotoxina Btk-HD-1 na dosagem de 1500 mg/kg de solo e solo autoclavado diferiram significativamente (P<0.05) da dose zero. Os pesos dos grãos nos tratamentos com a endotoxina Btk-HD-1 dosagem de 1500 mg/kg de solo e solo autoclavado foram respectivamente 61 e 85 % maiores. Nenhuma das endotoxinas usadas neste estudo reduziu significativamente (P<0,05) a população final de nematóides em relação o controle. ABSTRACT: In a greenhouse pot experiment, efficiency of endotoxins obtained from pure cultures of Bacillus sphaericus (Bs 2362), B. thuringiensis var. israelensis (Bti-H-14) and B. thuringiensis var. kurstaki (Btk-Hd-1) was evaluated against soybean cyst nematode, Heterodera glycines on soybean cv. Cristalina. Five doses (0, 250, 500, 1000 and 1500 mg) of each toxin with 1.108 spores per gram and 150 mg aldicarb were incorporated in upper 2-cm soil layer followed by application of 100 mL distilled water/kg of soil. Autoclaved soil was used as control for comparing plant growth with treatments. The experimental design was a completely randomized, with five replicates. Ten days after the application of endotoxins, a three-day after emergence soybean seedling was transplanted in each pot. Eighty-three days later, the increase in grain weight/plant and final populations of soybean cyst nematodes were evaluated. In general, grain production was very low. Grain weights for treatments with endotoxin Btk-HD-1 dose 1500 mg/Kg of soil and autoclaved soil differed significantly (P<0.05) from the control, yielding respectively 61% and 85 % higher than the control. None of the endotoxins used in this study could significantly (P<0.05) reduce the final nematode populations in relation to the untreated control (autoclaved soil).
650 $aBacillus sphaericus
650 $abiological control
650 $aNematoda
650 $asoybeans
650 $aBactéria
650 $aCisto
650 $aControle Biológico
650 $aHeterodera Glycines
650 $aNematóide
650 $aSoja
653 $aBacterial endotoxins
653 $aBiocontrol
653 $aIsraelensis
653 $aKurstaki
653 $aNematóide do cisto
653 $aNematóide-do-cisto
653 $aNematóide-do-cisto da soja
653 $aSoybean cyst nematode
653 $athuringiensis var
700 1 $aMOREIRA, W. A.
700 1 $aALVES, R. T.
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Registro original: |
Embrapa Cerrados (CPAC) |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Pecuária Sudeste. |
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Data corrente: |
08/09/2020 |
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Data da última atualização: |
10/09/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
AFONSO, J.; FORTES, M. R. S.; REVERTER, A.; DINIZ, W. J. da S.; CESAR, A. S. M.; LIMA, A. O. de; PETRINI, J.; SOUZA, M. de S.; COUTINHO, L. L.; MOURÃO, G. B.; ZERLOTINI NETO, A.; GROMBONI, C. F.; NOGUEIRA, A. R. A.; REGITANO, L. C. de A. |
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Afiliação: |
JULIANA AFONSO, UFSCar; MARINA RUFINO SALINAS FORTES, The University of Queensland; ANTONIO REVERTER, Agriculture and Food, Commonwealth Scientific and Industrial Research Organisation, Brisbane; WELLISON JARLES DA SILVA DINIZ, UFSCar; ALINE SILVA MELLO CESAR, Esalq/USP; ANDRESSA OLIVEIRA DE LIMA, UFSCar; JULIANA PETRINI, UNIFAL; MARCELA M. DE SOUZA, Iowa State University; LUIZ LEHMANN COUTINHO, Esalq/USP; GERSON BARRETO MOURÃO, Esalq/USP; ADHEMAR ZERLOTINI NETO, CNPTIA; CAIO FERNANDO GROMBONI, Bahia Federal Institute of Education, Science and Technology, Ilhéus; ANA RITA ARAÚJO NOGUEIRA; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
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Título: |
Genetic regulators of mineral amount in Nelore cattle muscle predicted by a new co-expression and regulatory impact factor approach. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Scientific Reports, v. 10, n. 1, p. 1-16, 2020 |
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DOI: |
https://doi.org/10.1038/s41598-020-65454-7 |
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Idioma: |
Inglês |
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Notas: |
Article: 8436. |
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Conteúdo: |
Mineral contents in bovine muscle can affect meat quality, growth, health, and reproductive traits. To better understand the genetic basis of this phenotype in Nelore (Bos indicus) cattle, we analysed genome-wide mRNA and miRNA expression data from 114 muscle samples. The analysis implemented a new application for two complementary algorithms: the partial correlation and information theory (PCIT) and the regulatory impact factor (RIF), in which we included the estimated genomic breeding values (GEBVs) for the phenotypes additionally to the expression levels, originally proposed for these methods. We used PCIT to determine putative regulatory relationships based on significant associations between gene expression and GEBVs for each mineral amount. Then, RIF was adopted to determine the regulatory impact of genes and miRNAs expression over the GEBVs for the mineral amounts. We also investigated over-represented pathways, as well as pieces of evidences from previous studies carried in the same population and in the literature, to determine regulatory genes for the mineral amounts. For example, NOX1 expression level was positively correlated to Zinc and has been described as Zinc-regulated in humans. Based on our approach, we were able to identify genes, miRNAs and pathways not yet described as underlying mineral amount. The results support the hypothesis that extracellular matrix interactions are the core regulator of mineral amount in muscle cells. Putative regulators described here add information to this hypothesis, expanding the knowledge on molecular relationships between gene expression and minerals. MenosMineral contents in bovine muscle can affect meat quality, growth, health, and reproductive traits. To better understand the genetic basis of this phenotype in Nelore (Bos indicus) cattle, we analysed genome-wide mRNA and miRNA expression data from 114 muscle samples. The analysis implemented a new application for two complementary algorithms: the partial correlation and information theory (PCIT) and the regulatory impact factor (RIF), in which we included the estimated genomic breeding values (GEBVs) for the phenotypes additionally to the expression levels, originally proposed for these methods. We used PCIT to determine putative regulatory relationships based on significant associations between gene expression and GEBVs for each mineral amount. Then, RIF was adopted to determine the regulatory impact of genes and miRNAs expression over the GEBVs for the mineral amounts. We also investigated over-represented pathways, as well as pieces of evidences from previous studies carried in the same population and in the literature, to determine regulatory genes for the mineral amounts. For example, NOX1 expression level was positively correlated to Zinc and has been described as Zinc-regulated in humans. Based on our approach, we were able to identify genes, miRNAs and pathways not yet described as underlying mineral amount. The results support the hypothesis that extracellular matrix interactions are the core regulator of mineral amount in muscle cells. Putative regulators describe... Mostrar Tudo |
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Palavras-Chave: |
Expressão gênica. |
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Thesagro: |
Bos Indicus; Gado Nelore. |
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Thesaurus NAL: |
Cattle; Gene expression. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/215819/1/AP-Genetic-regulators-2020.pdf
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Marc: |
LEADER 02716naa a2200361 a 4500
001 2124802
005 2020-09-10
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1038/s41598-020-65454-7$2DOI
100 1 $aAFONSO, J.
245 $aGenetic regulators of mineral amount in Nelore cattle muscle predicted by a new co-expression and regulatory impact factor approach.$h[electronic resource]
260 $c2020
500 $aArticle: 8436.
520 $aMineral contents in bovine muscle can affect meat quality, growth, health, and reproductive traits. To better understand the genetic basis of this phenotype in Nelore (Bos indicus) cattle, we analysed genome-wide mRNA and miRNA expression data from 114 muscle samples. The analysis implemented a new application for two complementary algorithms: the partial correlation and information theory (PCIT) and the regulatory impact factor (RIF), in which we included the estimated genomic breeding values (GEBVs) for the phenotypes additionally to the expression levels, originally proposed for these methods. We used PCIT to determine putative regulatory relationships based on significant associations between gene expression and GEBVs for each mineral amount. Then, RIF was adopted to determine the regulatory impact of genes and miRNAs expression over the GEBVs for the mineral amounts. We also investigated over-represented pathways, as well as pieces of evidences from previous studies carried in the same population and in the literature, to determine regulatory genes for the mineral amounts. For example, NOX1 expression level was positively correlated to Zinc and has been described as Zinc-regulated in humans. Based on our approach, we were able to identify genes, miRNAs and pathways not yet described as underlying mineral amount. The results support the hypothesis that extracellular matrix interactions are the core regulator of mineral amount in muscle cells. Putative regulators described here add information to this hypothesis, expanding the knowledge on molecular relationships between gene expression and minerals.
650 $aCattle
650 $aGene expression
650 $aBos Indicus
650 $aGado Nelore
653 $aExpressão gênica
700 1 $aFORTES, M. R. S.
700 1 $aREVERTER, A.
700 1 $aDINIZ, W. J. da S.
700 1 $aCESAR, A. S. M.
700 1 $aLIMA, A. O. de
700 1 $aPETRINI, J.
700 1 $aSOUZA, M. de S.
700 1 $aCOUTINHO, L. L.
700 1 $aMOURÃO, G. B.
700 1 $aZERLOTINI NETO, A.
700 1 $aGROMBONI, C. F.
700 1 $aNOGUEIRA, A. R. A.
700 1 $aREGITANO, L. C. de A.
773 $tScientific Reports$gv. 10, n. 1, p. 1-16, 2020
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Embrapa Agricultura Digital (CNPTIA) |
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