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| Registros recuperados : 1 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
22/11/2002 |
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Data da última atualização: |
08/11/2021 |
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Autoria: |
COUTINHO, H. L. da C.; OLIVEIRA, V. M. de; MANFIO, G. P.; ROSADO, A. S. |
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Afiliação: |
HEITOR LUIZ DA COSTA COUTINHO, CNPS. |
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Título: |
Evaluating the microbial diversity of soil samples: methodological innovations. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Anais da Academia Brasileira de Ciências, v. 71, n. 3, p. 491-503, 1999. |
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Idioma: |
Inglês |
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Conteúdo: |
This manuscript is a review of the innovative methodlogies that enable more precise evaluation of soil microbial diversity. Highlighting the molecular approach, which does not require the isolation of microorganisms and allows the inclusion of non-culturable genotypes in the analyses, the described methodologies revolutionised the environmental microbiology and opened gateways for an accurate understanding of the ecology and diversity of microorganisms. The application of techniques based on soil total DNA extraction, PCR amplification of genes or gene fragments, and sequence analysis revealed that the microbial universe in far more complex than ever imagined. Examples of applications of the molecular approach to study the diversity of soil diazotrophic bacteria are given. |
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Palavras-Chave: |
DGGE; Microbial diversity; Molecular methods; Soil DNA. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227018/1/Evaluating-the-microbial-diversity-of-soil-samples-1999.pdf
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Marc: |
LEADER 01401naa a2200205 a 4500
001 1336250
005 2021-11-08
008 1999 bl uuuu u00u1 u #d
100 1 $aCOUTINHO, H. L. da C.
245 $aEvaluating the microbial diversity of soil samples$bmethodological innovations.$h[electronic resource]
260 $c1999
520 $aThis manuscript is a review of the innovative methodlogies that enable more precise evaluation of soil microbial diversity. Highlighting the molecular approach, which does not require the isolation of microorganisms and allows the inclusion of non-culturable genotypes in the analyses, the described methodologies revolutionised the environmental microbiology and opened gateways for an accurate understanding of the ecology and diversity of microorganisms. The application of techniques based on soil total DNA extraction, PCR amplification of genes or gene fragments, and sequence analysis revealed that the microbial universe in far more complex than ever imagined. Examples of applications of the molecular approach to study the diversity of soil diazotrophic bacteria are given.
653 $aDGGE
653 $aMicrobial diversity
653 $aMolecular methods
653 $aSoil DNA
700 1 $aOLIVEIRA, V. M. de
700 1 $aMANFIO, G. P.
700 1 $aROSADO, A. S.
773 $tAnais da Academia Brasileira de Ciências$gv. 71, n. 3, p. 491-503, 1999.
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Embrapa Solos (CNPS) |
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