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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
26/04/2005 |
Data da última atualização: |
23/03/2023 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
SARTORATO, A.; NIETSCHE, S.; BARROS, E. G. de; MOREIRA, M. A. |
Afiliação: |
ALOISIO SARTORATO, CNPAF; SILVIA NIETSCHE, UFV; EVERALDO G. DE BARROS, UFV; MAURILIO A. MOREIRA, UFV. |
Título: |
Herança da resistência e marcadores RAPD ligados ao gene de resistência da cultivar México 54 ao patótipo 63-19 de Phaeoisariopsis griseola. |
Ano de publicação: |
1999 |
Fonte/Imprenta: |
In: REUNIÃO NACIONAL DE PESQUISA DE FEIJÃO, 6., 1999, Salvador. Resumos expandidos. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 1999. |
Volume: |
v.1. |
Páginas: |
p. 53-56. |
Série: |
(Embrapa Arroz e Feijão. Documentos, 99). |
ISSN: |
1516-7518 |
Idioma: |
Português |
Palavras-Chave: |
RAPD. |
Thesagro: |
Feijão; Gene; Marcador Molecular; Phaseolus Vulgaris; Resistência. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/213352/1/renafe-1999-p53.pdf
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Marc: |
LEADER 00875nam a2200241 a 4500 001 1213352 005 2023-03-23 008 1999 bl uuuu u00u1 u #d 022 $a1516-7518 100 1 $aSARTORATO, A. 245 $aHerança da resistência e marcadores RAPD ligados ao gene de resistência da cultivar México 54 ao patótipo 63-19 de Phaeoisariopsis griseola.$h[electronic resource] 260 $aIn: REUNIÃO NACIONAL DE PESQUISA DE FEIJÃO, 6., 1999, Salvador. Resumos expandidos. Santo Antônio de Goiás: Embrapa Arroz e Feijão$c1999 300 $ap. 53-56. v.1. 490 $a(Embrapa Arroz e Feijão. Documentos, 99).$vv.1. 650 $aFeijão 650 $aGene 650 $aMarcador Molecular 650 $aPhaseolus Vulgaris 650 $aResistência 653 $aRAPD 700 1 $aNIETSCHE, S. 700 1 $aBARROS, E. G. de 700 1 $aMOREIRA, M. A.
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Embrapa Arroz e Feijão (CNPAF) |
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Registro Completo
Biblioteca(s): |
Embrapa Amazônia Ocidental. |
Data corrente: |
14/02/2013 |
Data da última atualização: |
26/01/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
QUEIROZ, C. B.; MIRANDA, E. C.; HANADA, E. R.; SOUSA, N. R.; GASPAROTTO, L.; SOARES, M. A.; SILVA, G. F. |
Afiliação: |
NELCIMAR REIS SOUSA, CPAA; LUADIR GASPAROTTO, CPAA; GILVAN FERREIRA DA SILVA, CPAA. |
Título: |
Distribution of mating-type alleles and M13 PCR markers in the black leaf spot fungus Mycosphaerella fijiensis of bananas in Brazil. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 12, n. 1, p. 443-452, 2013. |
DOI: |
DOI: 10.4238/2013.February.8.8 |
Idioma: |
Inglês |
Conteúdo: |
The fungus Mycosphaerella fijiensis is the causative agent of black sigatoka, which is one of the most destructive diseases of banana plants. Infection with this pathogen results in underdeveloped fruit, with no commercial value. We analyzed the distribution of the M. fijiensis mating-type system and its genetic variability using M13 phage DNA markers. We found a 1:1 distribution of mating-type alleles, indicating MAT1-1 and MAT1-2 idiomorphs. A polymorphism analysis using three different primers for M13 markers showed that only the M13 minisatellite primers generated polymorphic products. We then utilized this polymorphism to characterize 40 isolates from various Brazilian states. The largest genetic distances were found between isolates from the same location and between isolates from different parts of the country. Therefore, there was no correlation between the genetic similarity and the geographic origin of the isolates. The M13 marker was used to generate genetic fingerprints for five isolates; these fingerprints were compared with the band profiles obtained from inter-simple sequence repeat (UBC861) and inter-retrotransposon amplified polymorphism analyses. We found that the M13 marker was more effective than the other two markers for differentiating these isolates. |
Palavras-Chave: |
IRAP; ISSR; Mating-type; Sigatoka-negra. |
Thesagro: |
Banana; Fungo; Mycosphaerella Fijiensis. |
Thesaurus NAL: |
Musa; polymorphism. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/76418/1/gmr2284.pdf
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Marc: |
LEADER 02182naa a2200313 a 4500 001 1949324 005 2018-01-26 008 2013 bl uuuu u00u1 u #d 024 7 $aDOI: 10.4238/2013.February.8.8$2DOI 100 1 $aQUEIROZ, C. B. 245 $aDistribution of mating-type alleles and M13 PCR markers in the black leaf spot fungus Mycosphaerella fijiensis of bananas in Brazil. 260 $c2013 520 $aThe fungus Mycosphaerella fijiensis is the causative agent of black sigatoka, which is one of the most destructive diseases of banana plants. Infection with this pathogen results in underdeveloped fruit, with no commercial value. We analyzed the distribution of the M. fijiensis mating-type system and its genetic variability using M13 phage DNA markers. We found a 1:1 distribution of mating-type alleles, indicating MAT1-1 and MAT1-2 idiomorphs. A polymorphism analysis using three different primers for M13 markers showed that only the M13 minisatellite primers generated polymorphic products. We then utilized this polymorphism to characterize 40 isolates from various Brazilian states. The largest genetic distances were found between isolates from the same location and between isolates from different parts of the country. Therefore, there was no correlation between the genetic similarity and the geographic origin of the isolates. The M13 marker was used to generate genetic fingerprints for five isolates; these fingerprints were compared with the band profiles obtained from inter-simple sequence repeat (UBC861) and inter-retrotransposon amplified polymorphism analyses. We found that the M13 marker was more effective than the other two markers for differentiating these isolates. 650 $aMusa 650 $apolymorphism 650 $aBanana 650 $aFungo 650 $aMycosphaerella Fijiensis 653 $aIRAP 653 $aISSR 653 $aMating-type 653 $aSigatoka-negra 700 1 $aMIRANDA, E. C. 700 1 $aHANADA, E. R. 700 1 $aSOUSA, N. R. 700 1 $aGASPAROTTO, L. 700 1 $aSOARES, M. A. 700 1 $aSILVA, G. F. 773 $tGenetics and Molecular Research$gv. 12, n. 1, p. 443-452, 2013.
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