Data/hora: 07/06/2024 02:14:55

Biblioteca(s): | Embrapa Instrumentação.
Data corrente: | 17/09/2021
Data da última atualização: | 10/06/2022
Autoria: | MARGARIDO, A.; MANZINE, L. R.; ARAUJO-MOREIRA, F. M.; GONÇALVES, R. V.; HERRMANN JUNIOR, P. S. de P.
Afiliação: | PAULO SERGIO DE P HERRMANN JUNIOR, CNPDIA.
Título: | Development of an enzyme-coated microcantilever-based biosensor for specific detection of short-chain alcohols.
Ano de publicação: | 2021
Fonte/Imprenta: | Engineering Proceedings, v. 6, n. 75 2021.
Páginas: | 1 - 5
ISSN: | 2359-1757
DOI: | https://doi.org/10.3390/I3S2021Dresden-10175
Idioma: | Inglês
Notas: | Presented at the 8th International Symposium on Sensor Science.
Conteúdo: | This paper describes the development of a biosensor designed for the enzymatic detection of short-chain alcohols. The biorecognition element, alcohol dehydrogenase, was immobilized on selfassembled monolayers deposited on top of silicon nitride microcantilevers. The self-assembly process was performed by surface activation using 3-aminopropyltriethoxysilane, followed by glutaraldehyde and biomolecule binding. X-ray photoelectron spectroscopy and atomic force microscopy were used. The biosensor showed a lower response time and sensibility from 0.03 to 1.2 mL/L. Its selectivity was analyzed through exposure to pure and mixed volatile solvents. Sensor sensibility was higher in the presence of short-chain alcohols and practically null involving other polar or nonpolar solvents.
Palavras-Chave: | Biorecognition element; Biosensor; Microcantilever.
Categoria do assunto: | W Química e Física
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/226141/1/P-Development-of-an-Enzyme-Coated-Microcantilever-Based-....pdf
Registro original: | Embrapa Instrumentação (CNPDIA)
Exemplares: | CNPDIA - (17837-1) - UPC - AP - DD - PROCI.21/88 -  - 2021/90 - DIS