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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
21/03/2011 |
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Data da última atualização: |
17/04/2018 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MORALES, A. M. A. P.; BOREM, A.; LOUREIRO, M.; MOREIRA, R. S.; ABDELNOOR, R. V.; GRAHAM, M. A. |
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Afiliação: |
AGUIDA MARIA ALVES PEREIRA MORALES, UFV; ALUÍZIO BOREM; MARCELO LOUREIRO; RENATA STOLF MOREIRA; RICARDO VILELA ABDELNOOR, CNPSO; MICHELLE A. GRAHAM, USDA-ARS. |
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Título: |
Expression analyses of candidate resistance genes in the Rpp4 Asian Soybean Rust resistance locus. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
2010. |
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Idioma: |
Inglês |
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Notas: |
Edição de Poster da 11. Annual National Outreach Scholarship Conference, Raleigh. |
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Conteúdo: |
Asian Soybean Rust (ASR), caused Phakopsora pachyrhizi, is considered the most severe soybean disease around the world. Infection of susceptible genotypes leads to early defoliation, incomplete seed development, and yield losses as high as 80%. Five ASR resistance genes have been identified in soybean: Rpp1, Rpp2, Rpp3, Rpp4 and Rpp5. Of particular interest is Rpp4, which has remained stable and confers resistance against P. pachyrhizi isolates from around the world. Rpp4 was mapped to soybean linkage group G (chromosome 18), 1.9 cM from simple sequence repeat (SSR) marker Satt288. Sequencing of this region in the susceptible genotype Williams 82 identified a cluster of three CC-NBS-LRR resistance genes. Virus Induced Gene Silencing was used to demonstrate that orthologous genes were responsible for resistance. We have now sequenced a >460 kb region of the Rpp4 locus in the resistant mapping parent PI459025B. Eight CC-NBS-LRR resistance genes have been identified in this region. In order to obtain more information about Rpp4 function, we are using real time quantitative PCR (qRT-PCR) to analyze the expression of all eight genes in different plant tissues, in different stages of development and after inoculation with P. pachyrhizi. We have developed a single pair of primers from the NBD domain that monitors the expression of all eight genes. Direct sequencing of the RT-PCR product differentiates between the eight genes. Detailed sequence analyses of the Rpp4 locus suggest that intra- and intergenic duplications and recombination have played an important role in creating genetic diversity. Alternative splicing of intragenic duplications may create additional sequence diversity at an RNA level. We are developing primers that will allow us to monitor alternative splicing events. Sequencing of the RT-PCR products will determine if alternative splicing plays a role in generating additional sequence diversity at the Rpp4 locus. MenosAsian Soybean Rust (ASR), caused Phakopsora pachyrhizi, is considered the most severe soybean disease around the world. Infection of susceptible genotypes leads to early defoliation, incomplete seed development, and yield losses as high as 80%. Five ASR resistance genes have been identified in soybean: Rpp1, Rpp2, Rpp3, Rpp4 and Rpp5. Of particular interest is Rpp4, which has remained stable and confers resistance against P. pachyrhizi isolates from around the world. Rpp4 was mapped to soybean linkage group G (chromosome 18), 1.9 cM from simple sequence repeat (SSR) marker Satt288. Sequencing of this region in the susceptible genotype Williams 82 identified a cluster of three CC-NBS-LRR resistance genes. Virus Induced Gene Silencing was used to demonstrate that orthologous genes were responsible for resistance. We have now sequenced a >460 kb region of the Rpp4 locus in the resistant mapping parent PI459025B. Eight CC-NBS-LRR resistance genes have been identified in this region. In order to obtain more information about Rpp4 function, we are using real time quantitative PCR (qRT-PCR) to analyze the expression of all eight genes in different plant tissues, in different stages of development and after inoculation with P. pachyrhizi. We have developed a single pair of primers from the NBD domain that monitors the expression of all eight genes. Direct sequencing of the RT-PCR product differentiates between the eight genes. Detailed sequence analyses of the Rpp4 locus suggest tha... Mostrar Tudo |
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Palavras-Chave: |
Ferrugem asiática da soja. |
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Thesagro: |
Doença fungica; Fungo. |
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Thesaurus Nal: |
Disease resistance; Fungal diseases of plants; Soybean rust. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/30153/1/abdelnoor.conf..pdf
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Marc: |
LEADER 02727nam a2200253 a 4500
001 1881697
005 2018-04-17
008 2010 bl uuuu u00u1 u #d
100 1 $aMORALES, A. M. A. P.
245 $aExpression analyses of candidate resistance genes in the Rpp4 Asian Soybean Rust resistance locus.
260 $a2010.$c2010
500 $aEdição de Poster da 11. Annual National Outreach Scholarship Conference, Raleigh.
520 $aAsian Soybean Rust (ASR), caused Phakopsora pachyrhizi, is considered the most severe soybean disease around the world. Infection of susceptible genotypes leads to early defoliation, incomplete seed development, and yield losses as high as 80%. Five ASR resistance genes have been identified in soybean: Rpp1, Rpp2, Rpp3, Rpp4 and Rpp5. Of particular interest is Rpp4, which has remained stable and confers resistance against P. pachyrhizi isolates from around the world. Rpp4 was mapped to soybean linkage group G (chromosome 18), 1.9 cM from simple sequence repeat (SSR) marker Satt288. Sequencing of this region in the susceptible genotype Williams 82 identified a cluster of three CC-NBS-LRR resistance genes. Virus Induced Gene Silencing was used to demonstrate that orthologous genes were responsible for resistance. We have now sequenced a >460 kb region of the Rpp4 locus in the resistant mapping parent PI459025B. Eight CC-NBS-LRR resistance genes have been identified in this region. In order to obtain more information about Rpp4 function, we are using real time quantitative PCR (qRT-PCR) to analyze the expression of all eight genes in different plant tissues, in different stages of development and after inoculation with P. pachyrhizi. We have developed a single pair of primers from the NBD domain that monitors the expression of all eight genes. Direct sequencing of the RT-PCR product differentiates between the eight genes. Detailed sequence analyses of the Rpp4 locus suggest that intra- and intergenic duplications and recombination have played an important role in creating genetic diversity. Alternative splicing of intragenic duplications may create additional sequence diversity at an RNA level. We are developing primers that will allow us to monitor alternative splicing events. Sequencing of the RT-PCR products will determine if alternative splicing plays a role in generating additional sequence diversity at the Rpp4 locus.
650 $aDisease resistance
650 $aFungal diseases of plants
650 $aSoybean rust
650 $aDoença fungica
650 $aFungo
653 $aFerrugem asiática da soja
700 1 $aBOREM, A.
700 1 $aLOUREIRO, M.
700 1 $aMOREIRA, R. S.
700 1 $aABDELNOOR, R. V.
700 1 $aGRAHAM, M. A.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 9 | |
| 1. |  | MORALES, A. M. A. P.; BORÉM, A.; GRAHAM, M.; ABDELNOOR, R. V. Advances on molecular studies of the interaction soybean - Asian rust. Crop Breeding Applied Biotechnology, Viçosa, MG, v. 12, n. 1, p. 1-7, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 2. | | PEREIRA, A. A.; MORALES, A. M. A. P.; BORÉM, A.; LOREIRO, M. E. Expressão de genes da subfamília HD-Zip I em soja submetida à seca. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 46, n. 8, p. 884-889, ago. 2011. Título em inglês: HD?Zip I subfamily gene expression in soybean subjected to drought.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 5. | | MORALES, A. M. A. P.; PEREIRA, A. A.; BRITO JUNIOR, S. L.; ROMERO, C. C.; BORÉM, A.; MARCELINO-GUIMARÃES, F. C.; GRAHAM, M. A.; ABDELNOOR, R. V. Caracterização molecular e microscópica da resistência à Ferrugem asiática da soja mediada pelo gene RPP4. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: anais. Brasília, DF: Embrapa, 2012. 4 p. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 6. | | MORALES, A. M. A. P.; PEREIRA, A. A.; LINCOLN, L.; FREEMAN, B. C.; BOREM, A.; ABDELNOOR, R. V.; GRAHAM, M. A. Transcriptome Analysis of Rpp4 Silenced Plants via virus-induced gene silencing (VIGS). In: BRAZILIAN SYMPOSIUM ON PLANT MOLECULAR BIOLOGY, 3., 2011, Ilhéus. [Abstracts...]. [Ribeirão Preto]: SBG, 2011. 1 p. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 7. | | MORALES, A. M. A. P.; LEMOS, E. G. M.; FUGANTI, R.; MARIN, S. R. R.; MARCELINO, F. C.; SILVA, J. F. V.; PEREIRA, A. A.; NEPOMUCENO, A. L. Expressão de genes envolvidos na resistência da soja a Meloidogyne javanica. Nematologia Brasileira, Piracicaba, v. 33, n. 3, p. 226-234, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Soja. |
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| 8. | | MORALES, A. M. A. P.; PEREIRA, A. A.; MURAKAMI, A. Y.; ROCHA, L. K. V.; STOLF, R.; BARBOSA, J. F.; ABDELNOOR, R. V.; MARCELINO, F. C.; BOREM, A.; LOUREIRO, M. E. Construção de biblioteca full length de soja inoculada com o fungo Phakopsora pachyrhizi. In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 12., 2009, Fortaleza. Desafios para a produção de alimentos e bioenergia: [anais...]. Fortaleza: UFC: Embrapa Agroindústria Tropical, 2009. Seção trabalhos, 15.pdf. 1 CD-ROM. CBFV 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 9. | | MORALES, A. M. A. P.; O'ROURKE, J. A.; MORTEL, M. van de; SCHEIDER, K. T.; BANCROFT, T. J.; BORÉM, A.; NELSON, R. T.; NETTLETON, D.; BAUM, T. J.; SHOEMAKER, R. C.; FREDERICK, R. D.; ABDELNOOR, R. V.; PEDLEY, K. F.; WHITHAM, S. A.; GRAHAM, M. A. Transcriptome analyses and virus induced gene silencing identify genes in the Rpp4-mediated Asian soybean rust resistance pathway. Functional Plant Biology, v. 40, n. 10, p. 1029-1047, sept. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 9 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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