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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
23/12/2015 |
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Data da última atualização: |
18/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MORAIS-LINO, L. S.; SEREJO, J. A. dos S.; AMORIM, E. P.; SANTANA, J. R. F. de; PASQUAL, M.; SILVA, S. de O. e. |
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Afiliação: |
LUCYMEIRE SOUZA MORAIS-LINO; JANAY ALMEIDA DOS SANTOS SEREJO, CNPMF; EDSON PERITO AMORIM, CNPMF; JOSÉ RANIERE FERREIRA DE SANTANA, UEFS; MOACIR PASQUAL, UFLA; SEBASTIÃO DE OLIVEIRA E SILVA, UFRB. |
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Título: |
Somatic embryogenesis, cell suspension, and genetic stability of banana cultivars. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In Vitro Cellular & Developmental Biology - Plant, November, 2015. |
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ISSN: |
1475-2689 |
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Idioma: |
Inglês |
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Conteúdo: |
The induction of somatic embryogenesis in banana is extremely difficult because of endogenous problems of the species and genotype dependency. Establishing a suitable protocol for somatic embryogenesis is necessary for applying biotechnological approaches to assist the genetic improvement, by facilitating the access to individual cells or groups of cells for use in genetic transformation, and the induction of polyploidy and utagenesis. Embryogenic cultures were induced from immature male flowers of two important cultivars of banana, ?Grand Naine? (AAA) and ?Tropical? (AAAB), using immature male flowers. Cell suspensions were established, and regenerated plants were evaluated for their genetic stability using 11 simple sequence repeat markers. For induction of embryogenesis, different doses of autoclaved glutamine in the induction medium were used, and somatic embryos were converted into plants in medium supplemented with benzylaminopurine and naphthalene-1-acetic acid. ?Grand Naine? formed somatic embryos in glutamine-free media, while ?Tropical? somatic embryos grew in the presence of autoclaved glutamine. During regeneration, ?Grand Naine? showed better results for formation of embryos in culture medium without growth regulators, but these embryos were not converted into plants when kept in the same medium, while ?Tropical? produced a large number of plants regenerated when kept in the same medium. The simple sequence repeat markers used did not detect any genetic variation. These results suggest that the establishment of embryogenic cell suspension cultures of banana may be effective for production of genetically stable plants on a large scale as well as being a biotechnological tool to support banana genetic improvement MenosThe induction of somatic embryogenesis in banana is extremely difficult because of endogenous problems of the species and genotype dependency. Establishing a suitable protocol for somatic embryogenesis is necessary for applying biotechnological approaches to assist the genetic improvement, by facilitating the access to individual cells or groups of cells for use in genetic transformation, and the induction of polyploidy and utagenesis. Embryogenic cultures were induced from immature male flowers of two important cultivars of banana, ?Grand Naine? (AAA) and ?Tropical? (AAAB), using immature male flowers. Cell suspensions were established, and regenerated plants were evaluated for their genetic stability using 11 simple sequence repeat markers. For induction of embryogenesis, different doses of autoclaved glutamine in the induction medium were used, and somatic embryos were converted into plants in medium supplemented with benzylaminopurine and naphthalene-1-acetic acid. ?Grand Naine? formed somatic embryos in glutamine-free media, while ?Tropical? somatic embryos grew in the presence of autoclaved glutamine. During regeneration, ?Grand Naine? showed better results for formation of embryos in culture medium without growth regulators, but these embryos were not converted into plants when kept in the same medium, while ?Tropical? produced a large number of plants regenerated when kept in the same medium. The simple sequence repeat markers used did not detect any genetic variatio... Mostrar Tudo |
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Palavras-Chave: |
Regulador de Cresimento. |
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Thesagro: |
Banana; Embriogenêse; Marcador molecular. |
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Thesaurus Nal: |
Growth regulators; Musa; Somatic embryos. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02560naa a2200277 a 4500
001 2032359
005 2023-05-18
008 2015 bl uuuu u00u1 u #d
022 $a1475-2689
100 1 $aMORAIS-LINO, L. S.
245 $aSomatic embryogenesis, cell suspension, and genetic stability of banana cultivars.$h[electronic resource]
260 $c2015
520 $aThe induction of somatic embryogenesis in banana is extremely difficult because of endogenous problems of the species and genotype dependency. Establishing a suitable protocol for somatic embryogenesis is necessary for applying biotechnological approaches to assist the genetic improvement, by facilitating the access to individual cells or groups of cells for use in genetic transformation, and the induction of polyploidy and utagenesis. Embryogenic cultures were induced from immature male flowers of two important cultivars of banana, ?Grand Naine? (AAA) and ?Tropical? (AAAB), using immature male flowers. Cell suspensions were established, and regenerated plants were evaluated for their genetic stability using 11 simple sequence repeat markers. For induction of embryogenesis, different doses of autoclaved glutamine in the induction medium were used, and somatic embryos were converted into plants in medium supplemented with benzylaminopurine and naphthalene-1-acetic acid. ?Grand Naine? formed somatic embryos in glutamine-free media, while ?Tropical? somatic embryos grew in the presence of autoclaved glutamine. During regeneration, ?Grand Naine? showed better results for formation of embryos in culture medium without growth regulators, but these embryos were not converted into plants when kept in the same medium, while ?Tropical? produced a large number of plants regenerated when kept in the same medium. The simple sequence repeat markers used did not detect any genetic variation. These results suggest that the establishment of embryogenic cell suspension cultures of banana may be effective for production of genetically stable plants on a large scale as well as being a biotechnological tool to support banana genetic improvement
650 $aGrowth regulators
650 $aMusa
650 $aSomatic embryos
650 $aBanana
650 $aEmbriogenêse
650 $aMarcador molecular
653 $aRegulador de Cresimento
700 1 $aSEREJO, J. A. dos S.
700 1 $aAMORIM, E. P.
700 1 $aSANTANA, J. R. F. de
700 1 $aPASQUAL, M.
700 1 $aSILVA, S. de O. e
773 $tIn Vitro Cellular & Developmental Biology - Plant, November, 2015.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
19/01/2012 |
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Data da última atualização: |
19/01/2012 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
RAMOS, T. C. D. A.; COTA, L. V.; COSTA, R. V.; SILVA, D. D. da; NOLASCO, A. A. R.; LANZA, F. E.; PARREIRA, D. F.; COSTA, G. M. C. |
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Afiliação: |
LUCIANO VIANA COTA, CNPMS; RODRIGO VERAS DA COSTA, CNPMS; DAGMA DIONISIA DA SILVA, CNPMS. |
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Título: |
Virulência de isolados de Exserohilum turcicum em genótipos de sorgo. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Tropical Plant Pathology, Brasília, DF, v. 36, p. 980, ago. 2011. |
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Idioma: |
Português |
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Notas: |
Suplemento. Edição dos resumos do 44º Congresso Brasileiro de Fitopatologia, 2011, Bento Gonçalves. Resumo 1404. |
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Thesagro: |
Doença de planta; Sorghum Bicolor. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/52564/1/Virulencia-isolados.pdf
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Marc: |
LEADER 00772nam a2200217 a 4500
001 1913028
005 2012-01-19
008 2011 bl uuuu u00u1 u #d
100 1 $aRAMOS, T. C. D. A.
245 $aVirulência de isolados de Exserohilum turcicum em genótipos de sorgo.$h[electronic resource]
260 $aTropical Plant Pathology, Brasília, DF, v. 36, p. 980, ago. 2011.$c2011
500 $aSuplemento. Edição dos resumos do 44º Congresso Brasileiro de Fitopatologia, 2011, Bento Gonçalves. Resumo 1404.
650 $aDoença de planta
650 $aSorghum Bicolor
700 1 $aCOTA, L. V.
700 1 $aCOSTA, R. V.
700 1 $aSILVA, D. D. da
700 1 $aNOLASCO, A. A. R.
700 1 $aLANZA, F. E.
700 1 $aPARREIRA, D. F.
700 1 $aCOSTA, G. M. C.
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Embrapa Milho e Sorgo (CNPMS) |
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