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Registro Completo |
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Biblioteca(s): |
Embrapa Amapá. |
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Data corrente: |
23/07/2012 |
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Data da última atualização: |
20/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VIDEIRA, S. S.; OLIVEIRA, D. M. DE; MORAIS, R. F.; BORGES, W. L.; BALDANI, V. L. D.; BALDANI, J. I. |
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Afiliação: |
SANDY SAMPAIO VIDEIRA, UFRRJ; DANILO MESSIAS DE OLIVEIRA, UFRRJ; RAFAEL FIUSA DE MORAIS, UFRRJ; WARDSSON LUSTRINO BORGES, CPAF-AP; VERA LUCIA DIVAN BALDANI, CNPAB; JOSE IVO BALDANI, CNPAB. |
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Título: |
Genetic diversity and plant growth promoting traits of diazotrophic bacteria isolated two Pennisetum purpureum Schum. Genotypes grown in the field. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Plant and Soil Online, v. 356, n. 1-2, 2012. |
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DOI: |
DOI 10.1007/s11104-011-1082-6 |
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Idioma: |
Inglês |
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Conteúdo: |
Background and aims Some elephant grass (Pennisetum purpureum) genotypes are able to produce large amounts of biomass and accumulate N derived from BNF when growing in soil with low N levels. However, information about the diazotrophic bacteria colonizing this C4 plant is still very scarce. This study aimed to characterize the plant growth promoting traits of a fraction of culturable diazotrophs colonizing the genotypes CNPGL F06-3 and Cameroon. Methods A total of 204 isolates were obtained from surface sterilized leaves, stems and roots after culturing on five different N-free semisolid media. These were then analyzed by BOX-PCR, and the 16S rRNA and nifH sequences of representative isolates were obtained. The functional ability of the isolates to reduce acetylene, produce indole and to solubilize phosphate was also determined. Results The diazotrophic bacterial population varied from 102 up to 106 bacteria g?1 fresh tissues of both genotypes. The BOX-PCR analysis suggested a trend in the genetic diversity among the 204 diazotrophic strains colonizing the different genotypes and plant tissues. Sequencing of 16S rRNA fragments confirmed the presence of Azospirillum brasilense and Gluconacetobacter diazotrophicus and revealed for the first time the occurrence of G. liquefaciens, G. sacchari, Burkholderia silvatlantica, Klebsiella sp., Enterobacter cloacae and E. oryzae in elephant grass. Interestingly, several nifH sequences from isolates identified as G. liquefaciens and G. sacchari showed homologies with nifH sequences of Enterobacter species. The majority of the isolates (97%) produced indole compounds, 22% solubilized phosphate and 6.4% possessed both characteristics. MenosBackground and aims Some elephant grass (Pennisetum purpureum) genotypes are able to produce large amounts of biomass and accumulate N derived from BNF when growing in soil with low N levels. However, information about the diazotrophic bacteria colonizing this C4 plant is still very scarce. This study aimed to characterize the plant growth promoting traits of a fraction of culturable diazotrophs colonizing the genotypes CNPGL F06-3 and Cameroon. Methods A total of 204 isolates were obtained from surface sterilized leaves, stems and roots after culturing on five different N-free semisolid media. These were then analyzed by BOX-PCR, and the 16S rRNA and nifH sequences of representative isolates were obtained. The functional ability of the isolates to reduce acetylene, produce indole and to solubilize phosphate was also determined. Results The diazotrophic bacterial population varied from 102 up to 106 bacteria g?1 fresh tissues of both genotypes. The BOX-PCR analysis suggested a trend in the genetic diversity among the 204 diazotrophic strains colonizing the different genotypes and plant tissues. Sequencing of 16S rRNA fragments confirmed the presence of Azospirillum brasilense and Gluconacetobacter diazotrophicus and revealed for the first time the occurrence of G. liquefaciens, G. sacchari, Burkholderia silvatlantica, Klebsiella sp., Enterobacter cloacae and E. oryzae in elephant grass. Interestingly, several nifH sequences from isolates identified as G. liquefaciens and G. ... Mostrar Tudo |
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Palavras-Chave: |
Elephant grass. |
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Thesagro: |
Bactéria; Capim elefante; Graminea forrageira. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/62277/1/AP-2012-Genetic-diversity-and-plant-growth-promoting.pdf
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Marc: |
LEADER 02473naa a2200241 a 4500
001 1928955
005 2018-06-20
008 2012 bl uuuu u00u1 u #d
024 7 $aDOI 10.1007/s11104-011-1082-6$2DOI
100 1 $aVIDEIRA, S. S.
245 $aGenetic diversity and plant growth promoting traits of diazotrophic bacteria isolated two Pennisetum purpureum Schum. Genotypes grown in the field.$h[electronic resource]
260 $c2012
520 $aBackground and aims Some elephant grass (Pennisetum purpureum) genotypes are able to produce large amounts of biomass and accumulate N derived from BNF when growing in soil with low N levels. However, information about the diazotrophic bacteria colonizing this C4 plant is still very scarce. This study aimed to characterize the plant growth promoting traits of a fraction of culturable diazotrophs colonizing the genotypes CNPGL F06-3 and Cameroon. Methods A total of 204 isolates were obtained from surface sterilized leaves, stems and roots after culturing on five different N-free semisolid media. These were then analyzed by BOX-PCR, and the 16S rRNA and nifH sequences of representative isolates were obtained. The functional ability of the isolates to reduce acetylene, produce indole and to solubilize phosphate was also determined. Results The diazotrophic bacterial population varied from 102 up to 106 bacteria g?1 fresh tissues of both genotypes. The BOX-PCR analysis suggested a trend in the genetic diversity among the 204 diazotrophic strains colonizing the different genotypes and plant tissues. Sequencing of 16S rRNA fragments confirmed the presence of Azospirillum brasilense and Gluconacetobacter diazotrophicus and revealed for the first time the occurrence of G. liquefaciens, G. sacchari, Burkholderia silvatlantica, Klebsiella sp., Enterobacter cloacae and E. oryzae in elephant grass. Interestingly, several nifH sequences from isolates identified as G. liquefaciens and G. sacchari showed homologies with nifH sequences of Enterobacter species. The majority of the isolates (97%) produced indole compounds, 22% solubilized phosphate and 6.4% possessed both characteristics.
650 $aBactéria
650 $aCapim elefante
650 $aGraminea forrageira
653 $aElephant grass
700 1 $aOLIVEIRA, D. M. DE
700 1 $aMORAIS, R. F.
700 1 $aBORGES, W. L.
700 1 $aBALDANI, V. L. D.
700 1 $aBALDANI, J. I.
773 $tPlant and Soil Online$gv. 356, n. 1-2, 2012.
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Registro original: |
Embrapa Amapá (CPAF-AP) |
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Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
26/10/2007 |
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Data da última atualização: |
26/10/2007 |
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Tipo da produção científica: |
Documentos |
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Circulação/Nível: |
-- - -- |
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Autoria: |
BARROSO, P. A. V.; HOFFMANN, L. |
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Título: |
Métodos de predição do comportamento de populações de melhoramento. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Campina Grande: Embrapa Algodão, 2003. |
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Páginas: |
38 p. |
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Série: |
(Documentos, 108) |
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ISSN: |
0103-0205 |
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Idioma: |
Português |
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Conteúdo: |
Algodão; Melhoramento genético |
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Palavras-Chave: |
Melhoramento genético. |
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Thesagro: |
Algodão. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPA/19713/1/DOC108.PDF
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Marc: |
LEADER 00524nam a2200181 a 4500
001 1275971
005 2007-10-26
008 2003 bl uuuu u0uu1 u #d
022 $a0103-0205
100 1 $aBARROSO, P. A. V.
245 $aMétodos de predição do comportamento de populações de melhoramento.
260 $aCampina Grande: Embrapa Algodão$c2003
300 $a38 p.
490 $a(Documentos, 108)
520 $aAlgodão; Melhoramento genético
650 $aAlgodão
653 $aMelhoramento genético
700 1 $aHOFFMANN, L.
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Registro original: |
Embrapa Algodão (CNPA) |
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