| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Agrobiologia. |
|
Data corrente: |
28/10/2019 |
|
Data da última atualização: |
28/10/2019 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
VIDEIRA, S. S.; OLIVEIRA, D. M. DE; MORAIS, R. F.; BORGES, W. L.; BALDANI, V. L. D.; BALDANI, J. I. |
|
Título: |
Genetic diversity and plant growth promoting traits of diazotrophic bacteria isolated two Pennisetum purpureum Schum. Genotypes grown in the field. |
|
Ano de publicação: |
2012 |
|
Fonte/Imprenta: |
Plant and Soil Online v. 356, n. 1-2, 2012. |
|
DOI: |
DOI 10.1007/s11104-011-1082-6 |
|
Idioma: |
Português |
|
Conteúdo: |
Background and aims Some elephant grass (Pennisetum purpureum) genotypes are able to produce large amounts of biomass and accumulate N derived from BNF when growing in soil with low N levels. However, information about the diazotrophic bacteria colonizing this C4 plant is still very scarce. This study aimed to characterize the plant growth promoting traits of a fraction of culturable diazotrophs colonizing the genotypes CNPGL F06-3 and Cameroon. Methods A total of 204 isolates were obtained from surface sterilized leaves, stems and roots after culturing on five different N-free semisolid media. These were then analyzed by BOX-PCR, and the 16S rRNA and nifH sequences of representative isolates were obtained. The functional ability of the isolates to reduce acetylene, produce indole and to solubilize phosphate was also determined. Results The diazotrophic bacterial population varied from 102 up to 106 bacteria g?1 fresh tissues of both genotypes. The BOX-PCR analysis suggested a trend in the genetic diversity among the 204 diazotrophic strains colonizing the different genotypes and plant tissues. Sequencing of 16S rRNA fragments confirmed the presence of Azospirillum brasilense and Gluconacetobacter diazotrophicus and revealed for the first time the occurrence of G. liquefaciens, G. sacchari, Burkholderia silvatlantica, Klebsiella sp., Enterobacter cloacae and E. oryzae in elephant grass. Interestingly, several nifH sequences from isolates identified as G. liquefaciens and G. sacchari showed homologies with nifH sequences of Enterobacter species. The majority of the isolates (97%) produced indole compounds, 22% solubilized phosphate and 6.4% possessed both characteristics. MenosBackground and aims Some elephant grass (Pennisetum purpureum) genotypes are able to produce large amounts of biomass and accumulate N derived from BNF when growing in soil with low N levels. However, information about the diazotrophic bacteria colonizing this C4 plant is still very scarce. This study aimed to characterize the plant growth promoting traits of a fraction of culturable diazotrophs colonizing the genotypes CNPGL F06-3 and Cameroon. Methods A total of 204 isolates were obtained from surface sterilized leaves, stems and roots after culturing on five different N-free semisolid media. These were then analyzed by BOX-PCR, and the 16S rRNA and nifH sequences of representative isolates were obtained. The functional ability of the isolates to reduce acetylene, produce indole and to solubilize phosphate was also determined. Results The diazotrophic bacterial population varied from 102 up to 106 bacteria g?1 fresh tissues of both genotypes. The BOX-PCR analysis suggested a trend in the genetic diversity among the 204 diazotrophic strains colonizing the different genotypes and plant tissues. Sequencing of 16S rRNA fragments confirmed the presence of Azospirillum brasilense and Gluconacetobacter diazotrophicus and revealed for the first time the occurrence of G. liquefaciens, G. sacchari, Burkholderia silvatlantica, Klebsiella sp., Enterobacter cloacae and E. oryzae in elephant grass. Interestingly, several nifH sequences from isolates identified as G. liquefaciens and G. ... Mostrar Tudo |
|
Palavras-Chave: |
Elephant grass. |
|
Thesagro: |
Bactéria; Capim elefante; Graminea forrageira. |
|
Categoria do assunto: |
S Ciências Biológicas |
|
Marc: |
LEADER 02473naa a2200241 a 4500
001 2113521
005 2019-10-28
008 2012 bl --- 0-- u #d
024 7 $aDOI 10.1007/s11104-011-1082-6$2DOI
100 1 $aVIDEIRA, S. S.
245 $aGenetic diversity and plant growth promoting traits of diazotrophic bacteria isolated two Pennisetum purpureum Schum. Genotypes grown in the field.$h[electronic resource]
260 $c2012
520 $aBackground and aims Some elephant grass (Pennisetum purpureum) genotypes are able to produce large amounts of biomass and accumulate N derived from BNF when growing in soil with low N levels. However, information about the diazotrophic bacteria colonizing this C4 plant is still very scarce. This study aimed to characterize the plant growth promoting traits of a fraction of culturable diazotrophs colonizing the genotypes CNPGL F06-3 and Cameroon. Methods A total of 204 isolates were obtained from surface sterilized leaves, stems and roots after culturing on five different N-free semisolid media. These were then analyzed by BOX-PCR, and the 16S rRNA and nifH sequences of representative isolates were obtained. The functional ability of the isolates to reduce acetylene, produce indole and to solubilize phosphate was also determined. Results The diazotrophic bacterial population varied from 102 up to 106 bacteria g?1 fresh tissues of both genotypes. The BOX-PCR analysis suggested a trend in the genetic diversity among the 204 diazotrophic strains colonizing the different genotypes and plant tissues. Sequencing of 16S rRNA fragments confirmed the presence of Azospirillum brasilense and Gluconacetobacter diazotrophicus and revealed for the first time the occurrence of G. liquefaciens, G. sacchari, Burkholderia silvatlantica, Klebsiella sp., Enterobacter cloacae and E. oryzae in elephant grass. Interestingly, several nifH sequences from isolates identified as G. liquefaciens and G. sacchari showed homologies with nifH sequences of Enterobacter species. The majority of the isolates (97%) produced indole compounds, 22% solubilized phosphate and 6.4% possessed both characteristics.
650 $aBactéria
650 $aCapim elefante
650 $aGraminea forrageira
653 $aElephant grass
700 1 $aOLIVEIRA, D. M. DE
700 1 $aMORAIS, R. F.
700 1 $aBORGES, W. L.
700 1 $aBALDANI, V. L. D.
700 1 $aBALDANI, J. I.
773 $tPlant and Soil Online$gv. 356, n. 1-2, 2012.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agrobiologia (CNPAB) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 62 | |
| 3. |  | GUIMARÃES, A. P.; MORAIS, R. F.; URQUIAGA, S.; BODDEY, M.; ALVES, B. J. Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixatinon in soybean Scientia Agricola, Piracicaba, v. 65, n. 5, p. 516-524, set./out. 2008. Parceria: UENF| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Agrobiologia. |
|    |
| 5. | | MORAIS, R. F.; SOUZA, B. J.; LEITE, J. M.; ALVES, B. J. R.; URQUIAGA, S. Estoque de carbono em solos cultivados com diferentes genótipos de capim elefante. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO, 18., 2008, Seropédica, RJ. Seropédica, RJ, 2008. Parceria: UFRRJ| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
| |
| 7. | | ALMEIDA, R. F. de; ALVES, B. J. R.; BATISTA, J. N.; MORAIS, R. F. de. Quantificação das emissões de N2O após uso de ureia em agrissolo Vermelho-Amarelo cultivado com milho. In: REUNIÃO BRASILEIRA DE CIÊNCIA DO SOLO E NUTRIÇÃO DE PLANTAS, 30.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 14.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 12.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 9.; SIMPÓSIO SOBRE SELÊNIO NO BRASIL, 1., 2012, Maceió. A responsabilidade socioambiental da pesquisa agrícola: anais. Viçosa, MG: SBCS, 2012. FERTBIO 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
| |
| 9. | | PONTES, F.; MORAIS, R. F. de; REIS, V. M.; BALDANI, J. I.; URQUIAGA, S. Efeito da inoculação com mistura de bactérias diazotróficas no rendimento de colmos de dois genótipos de cana-de-açúcar, em condições de campo. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA UFRRJ, 17., 2007, Seropédica, RJ. Anais... Seropédica: Editora da UFRRJ, 2007. 2 p. 1 CD-ROM. Parcerias: UFRRJ.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Agrobiologia. |
| |
| 10. | | MORAIS, R. F. de; XAVIER, R. P.; ALVES, B. J. R.; BODDEY, R. M.; URQUIAGA, S. Uso da abundância de 15N (delta 15N) no perifil do solo como suporte para a estimativa da FBN. Seropédica: Embrapa Agrobiologia, 2006. 4 p. Trabalho apresentado na XXVII Reunião Brasileira de Fertilidade do Solo e Nutrição de Plantas, XI Reunião Brasileira sobre Micorrizas, IX Simpósio Brasileiro de Microbiologia do Solo; VI Reunião Brasileira de Biologia do Solo, Bonito/MS,...| Biblioteca(s): Embrapa Agrobiologia. |
| |
| 13. | | MORAIS, R. F.; XAVIER, R. P.; LIMA, R. M.; ALVES, B. J. R.; BODDEY, R. M.; URQUIAGA, S. Avaliação da contribuição da FBN em 9 variedades de cana-de-açúcar. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 30., 2005, Recife. Solos: sustentabilidade e qualidade ambiental. Recife: Sociedade Brasileira de Ciência do Solo, 2005. 4 p. CD ROM.| Biblioteca(s): Embrapa Agrobiologia. |
| |
| 14. | | BATISTA, J. N.; ALMEIDA, R. F.; MORAIS, R. F. de; JANTALIA, C. P.; ALVES, B. J. R.; URQUIAGA, S. Avaliação das perdas de nitrogênio pela aplicação de ureia convencional e granulada com tecnologia agregadas. In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 12., 2012. Economia verde, sustentabilidade e erradicação da pobreza: caderno de resumos. Seropédica: Embrapa Agrobiologia, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
|  |
| 17. | | MORAIS, R. F.; QUESADA, D. M.; REIS, V. M.; URQUIAGA, S.; ALVES, B. J. R.; BODDEY, R. M. Contribution of biological nitrogen fixation to elephant grass (Pennisetum purpureum Schum.). Plant and Soil Online, 14 sep. 2011. Publicado no volume impresso 356, Issue 1-2, pp 23-34, July 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia. |
| |
| 18. | | MORAIS, R. F.; ZANETTI, J. B.; ALVES, B. J. R.; BODDEY, R. M.; CABALLERO, S. S. U. The benefit of Biological Nitrogen Fixation to Facilitate the use elephant Grass (pennisetum purpureum schum.) as an Alternative Energy source. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. SVII.08| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
| |
| Registros recuperados : 62 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
