Data/hora: 12/06/2024 02:29:09

Biblioteca(s): | Embrapa Agrobiologia.
Data corrente: | 13/08/2019
Data da última atualização: | 26/11/2019
Autoria: | FRANCISCO, R.; BRANCO, R.; SCHWAB, S.; BALDANI, J. I.; MORAIS, P. V.
Afiliação: | Romeu Francisco, University of Coimbra; Rita Branco, University of Coimbra; STEFAN SCHWAB, CNPAB; JOSE IVO BALDANI, CNPAB; Paula V. Morais1,2, BOLSISTA EMBRAPA AGROBIOLOGIA.
Título: | Two plant hosted whole cell bacterial biosensors for detection of bioavailable Cr(VI).
Ano de publicação: | 2019
Fonte/Imprenta: | World Journal of Microbiology and Biotechnology, v. 35, n. p. 129-143, 2019.
DOI: | https://doi.org/10.1007/s11274-019-2703-0
Idioma: | Inglês
Conteúdo: | Metal whole cell biosensors (WCBs) have been reported as very useful tools to detect and quantify the presence of bioavailable fractions of certain metals in water and soil samples. In the current work, two bacterial WCBs able to report Cr(VI) presence and plants growing on Cr(VI)-enriched soil/medium were used to assess the potential transfer of this metal to organisms of higher trophic levels, and the risk of transfer to the food chain. To do it, the functionality of the WCBs within tissues of inoculated plants in contact with Cr(VI)-contaminated soil and water was studied in vitro and in a controlled greenhouse environment. One WCB was the previously described Ochrobactrum tritici pCHRGFP2 and the second, Nitrospirillum amazonense pCHRGFP2, is a newly engineered naturally-occurring endophytic microorganism. Three rice varieties (IAC 4440, BRS 6 CHUÍ, IRGA 425) and one maize variety (1060) were tested as hosts and subjected to Cr(VI) treatments (25 ?M), with different results obtained. Inoculation of each WCB into plants exposed to Cr(VI) showed GFP expression within plant tissues. WCBs penetrated the root tissues and later colonized the shoots and leaves. In general, a higher fluorescence signal was detected in roots, together with a higher Cr content and denser WCB colonization. Best fluorescence intensities per plant biomass of shoots were obtained for plant host IRGA 425. Therefore, by analyzing colonized tissues, both WCBs allowed the detection of Cr(VI) contamination in soils and its transfer to plants commonly used in crops for human diet.
Palavras-Chave: | Nitrospirillum amazonense; Wholecell biosensors.
Thesaurus Nal: | Chromium; Rice soils.
Categoria do assunto: | S Ciências Biológicas
Registro original: | Embrapa Agrobiologia (CNPAB)
Exemplares: | CNPAB - (41203-1) - UPC - AP - DD -  -  -  - DIS