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Registro Completo |
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
08/02/2018 |
Data da última atualização: |
08/02/2018 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
FERREIRA JUNIOR, O. L.; OLIVEIRA, R. D. de; MELO, I. S. de; MORAES, L. A. B. |
Afiliação: |
O. L. FERREIRA JUNIOR; R. D. de OLIVEIRA; ITAMAR SOARES DE MELO, CNPMA; L. A. B. MORAES. |
Título: |
Effects of different culture medium on albocycline and analogous production by Streptomyces sp. from caatinga biome. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
In: BRAZILIAN CONFERENCE ON NATURAL PRODUCTS, 6.; ANUAL MEETING ON MICROMOLECULAR EVOLUTION, SYSTEMATICS AND ECOLOGY, 32., 2017. Vitória. Annals... Vitória: UFES, 2017. |
Idioma: |
Inglês |
Conteúdo: |
The Albocycline (Fig. A), a macrocyclic lactone knowing by their antimicrobial activity, however it is poorly explored by its biological activity, for example fitotoxic activity. Despite the urgency for discoveries in the natural pesticides and agro-chemicals field, this study has goals the production of albocycline and its analogous from fermentation process. Furthermore, the compounds showed a fitotoxic activity against Lemna minor. A Streptomyces sp. - CAAT 7-52 strain isolated from Caatinga, an unique Brazilian biome, was cultivated in five different types of liquid medium: PD (Potato-Dextrose), PMB (Beef extract, yeast extract, dextrose, tryptose, starch, CaCO3, trace solution), ISP-2 (Yeast extract, malt extract, dextrose), Czapeck (CZ) (Sucrose, K2HPO4, MgSO4, FeSO4, NaNO3) and a modified Czapeck (CZ-GL) with sucrose replaced by glucose syrup for 7 days at 130 rpm and 30° C. The secondary metabolites were extracted with ethyl acetate and characterized LC-MS/MS system (Acquity Xevo TQ-S, Waters Corp.). Activity against Lemna minor was obtained by bioassay in SIS (Swedish Standard) medium for 7 days incubated in photoperiod chamber at 28° C, using Atrazine as positive control and DMSO e SIS medium as negative controls. Bioautography assay used TLC with ethyl acetate / hexane (1:1) as mobile phase, covered with agar and Lemna minor placed in the fractions zones. Atrazine was the positive control and agar the negative control. The chromatogram (Fig. B) and mass spectra analyses showed a pattern of peaks assigned to albocycline (m/z 639 [2M+Na+]+, m/z 331 [M+Na+]+ and m/z 309 [M+H+]+) also its analogous forms all isomers with the molecular mass 324.2 Da (m/z 347 [M+Na+]+ and m/z 671 [2M+Na+]+) which also revealed the same activity as albocycline. Accordant to the chromatogram and mass spectra analyses, albocycline was present in the extracts come from PD, PMB and CZ-GL. The analogous was detected only in the PD and ISP-2 extract and the CZ extract didn?t produce any compounds. The extracts were them summited to bioassay against the aquatic plant Lemna minor and all the extracts (with exception for CZ) exhibited a strong fitotoxic activity by the necrosis of the plant leaves. Therefore, was observed how the nutrient medium composition changes the secondary metabolites biosynthesis and how it can affect the extract bioactivity, as well a strong fitotoxic activity with no registers in literature. MenosThe Albocycline (Fig. A), a macrocyclic lactone knowing by their antimicrobial activity, however it is poorly explored by its biological activity, for example fitotoxic activity. Despite the urgency for discoveries in the natural pesticides and agro-chemicals field, this study has goals the production of albocycline and its analogous from fermentation process. Furthermore, the compounds showed a fitotoxic activity against Lemna minor. A Streptomyces sp. - CAAT 7-52 strain isolated from Caatinga, an unique Brazilian biome, was cultivated in five different types of liquid medium: PD (Potato-Dextrose), PMB (Beef extract, yeast extract, dextrose, tryptose, starch, CaCO3, trace solution), ISP-2 (Yeast extract, malt extract, dextrose), Czapeck (CZ) (Sucrose, K2HPO4, MgSO4, FeSO4, NaNO3) and a modified Czapeck (CZ-GL) with sucrose replaced by glucose syrup for 7 days at 130 rpm and 30° C. The secondary metabolites were extracted with ethyl acetate and characterized LC-MS/MS system (Acquity Xevo TQ-S, Waters Corp.). Activity against Lemna minor was obtained by bioassay in SIS (Swedish Standard) medium for 7 days incubated in photoperiod chamber at 28° C, using Atrazine as positive control and DMSO e SIS medium as negative controls. Bioautography assay used TLC with ethyl acetate / hexane (1:1) as mobile phase, covered with agar and Lemna minor placed in the fractions zones. Atrazine was the positive control and agar the negative control. The chromatogram (Fig. B) and mass spectra an... Mostrar Tudo |
Thesagro: |
Caatinga. |
Thesaurus Nal: |
Streptomyces. |
Categoria do assunto: |
V Taxonomia de Organismos |
Marc: |
LEADER 03135naa a2200181 a 4500 001 2087489 005 2018-02-08 008 2017 bl --- 0-- u #d 100 1 $aFERREIRA JUNIOR, O. L. 245 $aEffects of different culture medium on albocycline and analogous production by Streptomyces sp. from caatinga biome.$h[electronic resource] 260 $c2017 520 $aThe Albocycline (Fig. A), a macrocyclic lactone knowing by their antimicrobial activity, however it is poorly explored by its biological activity, for example fitotoxic activity. Despite the urgency for discoveries in the natural pesticides and agro-chemicals field, this study has goals the production of albocycline and its analogous from fermentation process. Furthermore, the compounds showed a fitotoxic activity against Lemna minor. A Streptomyces sp. - CAAT 7-52 strain isolated from Caatinga, an unique Brazilian biome, was cultivated in five different types of liquid medium: PD (Potato-Dextrose), PMB (Beef extract, yeast extract, dextrose, tryptose, starch, CaCO3, trace solution), ISP-2 (Yeast extract, malt extract, dextrose), Czapeck (CZ) (Sucrose, K2HPO4, MgSO4, FeSO4, NaNO3) and a modified Czapeck (CZ-GL) with sucrose replaced by glucose syrup for 7 days at 130 rpm and 30° C. The secondary metabolites were extracted with ethyl acetate and characterized LC-MS/MS system (Acquity Xevo TQ-S, Waters Corp.). Activity against Lemna minor was obtained by bioassay in SIS (Swedish Standard) medium for 7 days incubated in photoperiod chamber at 28° C, using Atrazine as positive control and DMSO e SIS medium as negative controls. Bioautography assay used TLC with ethyl acetate / hexane (1:1) as mobile phase, covered with agar and Lemna minor placed in the fractions zones. Atrazine was the positive control and agar the negative control. The chromatogram (Fig. B) and mass spectra analyses showed a pattern of peaks assigned to albocycline (m/z 639 [2M+Na+]+, m/z 331 [M+Na+]+ and m/z 309 [M+H+]+) also its analogous forms all isomers with the molecular mass 324.2 Da (m/z 347 [M+Na+]+ and m/z 671 [2M+Na+]+) which also revealed the same activity as albocycline. Accordant to the chromatogram and mass spectra analyses, albocycline was present in the extracts come from PD, PMB and CZ-GL. The analogous was detected only in the PD and ISP-2 extract and the CZ extract didn?t produce any compounds. The extracts were them summited to bioassay against the aquatic plant Lemna minor and all the extracts (with exception for CZ) exhibited a strong fitotoxic activity by the necrosis of the plant leaves. Therefore, was observed how the nutrient medium composition changes the secondary metabolites biosynthesis and how it can affect the extract bioactivity, as well a strong fitotoxic activity with no registers in literature. 650 $aStreptomyces 650 $aCaatinga 700 1 $aOLIVEIRA, R. D. de 700 1 $aMELO, I. S. de 700 1 $aMORAES, L. A. B. 773 $tIn: BRAZILIAN CONFERENCE ON NATURAL PRODUCTS, 6.; ANUAL MEETING ON MICROMOLECULAR EVOLUTION, SYSTEMATICS AND ECOLOGY, 32., 2017. Vitória. Annals... Vitória: UFES, 2017.
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Embrapa Meio Ambiente (CNPMA) |
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61. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | CUNHA, L. C. V.; NAGATA, T.; RESENDE, R. O.; INOUE-NAGATA, A. K. Biological, serological and genomic characteristics of a potyvirus isolated from tomato in Brazil. Virus Reviews & Research, Florianópolis, v. 6, n. 2, p. 154- 155, 2001. Resumo. Suplemento 1. Trabalho apresentado no 12º National Meeting of Virology, 4º Mercusul Meeting of Virology, Caldas Novas, 2001.Biblioteca(s): Embrapa Hortaliças. |
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62. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | LEMOS, P. P. F.; ALMEIDA, M. M. S.; MOITA, A. W.; INOUE-NAGATA, A. K. Análise da época de inoculação de begomovírus na susceptbilidade e produtividade de tomateiro. Tropical Plant Pathology, Brasília, DF, v. 34, p. S138, ago. 2009. Suplemento. Resumo 502. Trabalho apresentado no 42. Congresso Brasileiro de Fitopatologia, Rio de Janeiro.Tipo: Resumo em Anais de Congresso |
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63. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | CORREIA, L. Q.; FERREIRA, P. T. O.; DUSI, A. N.; INOUE-NAGATA, A. K. Análise espacial e temporal da ocorrência de begomovírus em tomateiro no DF. Fitopatologia Brasileira, Brasília, DF, v. 31, p. S245, ago. 2006. Suplemento. Resumo 0494. Trabalho apresentado no 39. Congresso Brasileiro de Fitopatologia, 2006, Salvador.Biblioteca(s): Embrapa Hortaliças. |
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66. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | INOUE-NAGATA, A. K.; ALBUQUERQUE, L. C.; ROCHA, W. B.; NAGATA, T. Cloning of the begomovirus complete genome using the bacteriophage 29 polymerase. INTERNATIONAL GERMINIVIRUS SYMPOSIUM, 4.; INTERNATIONAL SSDNA COMPARATIVE VIROLOGY WORKSHOP, 2., 2004, Cape Town, Programme & abstracts... Cape Town: University of Cape Town Graduate School of Business, 2004. p. 100Biblioteca(s): Embrapa Hortaliças. |
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67. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ALBUQUERQUE, L. C.; ROCHA, W. B.; NAGATA, T.; INOUE-NAGATA, A. K. Cloning of the complete genome of a begomovirus using the bacteriophage 29 DNA polymerase. Virus: Reviews & Research, Florianópolis, v. 8, p. 188, set. 2003. Suplemento 1. Trabalho apresentado no 14. National Meeting of Virology, 2003, Florianópolis, SC, Brasil. Resumo.Biblioteca(s): Embrapa Hortaliças. |
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68. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | PAPROTKA, T.; INOUE-NAGATA, A. K.; RESENDE, R.; JESKE, H.; RIBEIRO, S. G. Cloning and molecular analysis of sweet potato begomoviruses with Rolling circle amplication. Tropical Plant Pathology, Brasília, DF, v. 33, p. S292, ago. 2008. Suplemento. Resumo VIR 028. Trabalho apresentado no 41. Congresso Brasileiro de Fitopatologia, 41. Annual Meeting of the Brazilian Phytopathological Society, Belo Horizonte, 2008.Tipo: Resumo em Anais de Congresso |
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71. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | NAGATA, T.; RESENDE, R. O.; INOUE-NAGATA, A. K.; ÁVILA, A. C. de. The fluctuation of transmission specificity and efficiency of tomato spotted wilt virus by Frankliniella schultzei. Fitopatologia Brasileira, Brasília, DF, v. 32, n. 5, p. 439, set./ out. 2007.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
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76. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | DIANESE, E. C.; BOITEUX, L. S.; INOUE-NAGATA, A. K.; RESENDE, R. O. Identificação de novas fontes de resistência ao Pepper yellow mosaic virus em espécies selvagens de Solanum (Secção Lycopersicon). Horticultura Brasileira, Brasilia, DF, v. 26, n. 2, p. S4855-S4861 2008. Suplemento. CD-ROM. Trabalho apresentado no 48. Congresso Brasileiro de Olericultura, Maringá, 2008.Tipo: Artigo em Anais de Congresso / Nota Técnica |
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78. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MACEDO, M. A.; MICHEREFF FILHO, M.; NOVAS-CASTILLO, J.; INOUE-NAGATA, A. K. Host range and whitefly transmission efficiency of Tomato severe rugose virus and Tomato golden vein virus in tomato plants. Tropical Plant Pathology, Brasília, DF, v. 40, n. 6, p. 405-409, Dec. 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
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