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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
11/01/2018 |
Data da última atualização: |
27/01/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ASCARI, I. J.; ALVES, N. G.; JASMIN, J.; LIMA, R. R.; QUINTAO, C. C. R.; OBERLENDER, G.; MORAES, E. A.; CAMARGO, L. S. de A. |
Afiliação: |
I. J. ASCARI, UFLA; N. G. ALVES, UFLA; J. JASMIN, UFRJ; Renato Ribeiro Lima, UFLA; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; G. OBERLENDER, IEF Muzambinho, MG; E. A. MORAES, Universidade Federal do Vale do São Francisco; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
Título: |
Addition of insulin-like growth factor I to the maturation medium of bovine oocytes subjected to heat shock: effects on the production of reactive oxygen species, mitochondrial activity and oocyte competence. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Domestic Animal Endocrinology, v. 60, p. 50-60, 2017. |
DOI: |
10.1016/j.domaniend.2017.03.003 |
Idioma: |
Inglês |
Conteúdo: |
Abstract This study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 h at 38.5C, or heat shock: 12 h at 41C followed by 12 h at 38.5C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM mediumwith IGF-I may increase the mitochondrial membrane potential and ROS production in oocytes and decrease apoptosis in the ICM in blastocysts. Heat shock for 12 h compromised oocyte developmental competence and increased apoptosis within the ICM cells of the blastocysts. MenosAbstract This study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 h at 38.5C, or heat shock: 12 h at 41C followed by 12 h at 38.5C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM mediumwith IGF-I may inc... Mostrar Tudo |
Palavras-Chave: |
Mitochondrial membrane potential; ROS production; Thermoprotection. |
Thesaurus Nal: |
DNA fragmentation; heat stress. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02716naa a2200277 a 4500 001 2085044 005 2023-01-27 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1016/j.domaniend.2017.03.003$2DOI 100 1 $aASCARI, I. J. 245 $aAddition of insulin-like growth factor I to the maturation medium of bovine oocytes subjected to heat shock$beffects on the production of reactive oxygen species, mitochondrial activity and oocyte competence.$h[electronic resource] 260 $c2017 520 $aAbstract This study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 h at 38.5C, or heat shock: 12 h at 41C followed by 12 h at 38.5C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM mediumwith IGF-I may increase the mitochondrial membrane potential and ROS production in oocytes and decrease apoptosis in the ICM in blastocysts. Heat shock for 12 h compromised oocyte developmental competence and increased apoptosis within the ICM cells of the blastocysts. 650 $aDNA fragmentation 650 $aheat stress 653 $aMitochondrial membrane potential 653 $aROS production 653 $aThermoprotection 700 1 $aALVES, N. G. 700 1 $aJASMIN, J. 700 1 $aLIMA, R. R. 700 1 $aQUINTAO, C. C. R. 700 1 $aOBERLENDER, G. 700 1 $aMORAES, E. A. 700 1 $aCAMARGO, L. S. de A. 773 $tDomestic Animal Endocrinology$gv. 60, p. 50-60, 2017.
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Registros recuperados : 110 | |
5. | | SOUZA, W. L.; MORAES, E. A.; OLIVEIRA, R. P. de. Colesterol carregado pela ciclodextrina sobre a criopreservação dos espermatozoides de garanhões da raça Nordestina. Pesquisa Veterinária Brasileira, Brasília, DF, v. 38, n. 5, p. 991-996, maio, 2018.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Semiárido; Embrapa Unidades Centrais. |
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8. | | DEL PELOSO, M. J.; MORAES, E. A.; DUTRA, L. G. Efeito do parcelamento da adubação nitrogenada em cobertura na cultura do feijoeiro de inverno com irrigação por aspersão, e em várzea, no Estado de Goiás. In: REUNIÃO SOBRE FEIJAO IRRIGADO (GO, DF, MG, ES, SP, RJ), 1., 1988, Goiânia. Anais. Goiânia: EMBRAPA-CNPAF, 1990. p. 94-95. (EMBRAPA-CNPAF. Documentos, 27).Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Arroz e Feijão. |
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10. | | DUTRA, L. G.; DEL PELOSO, M. J.; MORAES, E. A. Uso do gesso agrícola para feijoeiro irrigado no Estado de Goiás. In: REUNIÃO SOBRE FEIJAO IRRIGADO (GO, DF, MG, ES, SP, RJ), 1., 1988, Goiânia. Anais. Goiânia: EMBRAPA-CNPAF, 1990. p. 98-100. (EMBRAPA-CNPAF. Documentos, 27).Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Arroz e Feijão. |
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11. | | MORAES, E. A. de; OLIVEIRA, J. E. de; MATTOSO, L. H. C.; MARTINS, M. A. Avaliação da liberação da rodamina-B em borracha natural. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 4., 2012, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação: Embrapa Pecuária Sudeste, 2012. p. 67. Editores técnicos: João de Mendonça Naime, Lucimara Aparecida Forato, Maria Alice Martins, Ladislau Marcelino Rabello, Rubens Bernardes Filho. (Embrapa Instrumentação. Documentos, 56).Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
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18. | | MORAES, E. A.; OLIVEIRA, J. E.; MEDEIROS, E. S.; MATTOSO, L. H. C. Fibers of biodegradable blend polymer obtained by Solution Blow Spinning In: INTERNATIONAL CONFERENCE ON FOOD AND AGRICULTURE APPLICATIONS OF NANOTECHNOLOGIES - NanoAgri, 2010, São Pedro, SP. [Anais…] São Pedro: Aptor Software, 2010. Editors: Caue Ribeiro, Odílio Benedito Garrido de Assis, Luiz Henrique Capparelli Mattoso, Sérgio Mascarenhas.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
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Registros recuperados : 110 | |
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