Data/hora: 11/06/2024 16:53:22

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 23/05/2000
Data da última atualização: | 03/08/2023
Autoria: | TACHEDJIAN, M.; KRYWULT, J.; MOORE, R. J.; HODGON, A. L. M.
Título: | Caseous lymphadenitis vaccine development: site-specific inactivation of the Corynebacterium pseudotuberculosis phospholipase D gene.
Ano de publicação: | 1995
Fonte/Imprenta: | Vaccine, v. 13, n. 18, p. 1785-1792, Dec. 1995.
DOI: | 10.1016/0264-410x(95)00144-p.
Idioma: | Inglês
Conteúdo: | Abstract: Vaccines for ovine caseous lymphadenitis (CLA) are currently formulated using partially purified, formalin inactivated phospholipase D (PLD) derived from Corynebacterium pseudotuberculosis culture supernatants. Chemical treatment has been a common and effective way of inactivating bacterial toxins for use in toxoid vaccines. Genetic inactivation of toxin genes using site-specific mutagenesis has the potential to improve this process by providing a safer and more cost-effective product. In the present study amino acid substitutions at the putative catalytic site and metal binding domain of the PLD protein had a profound affect upon PLD activity and secretion from C. pseudotuberculosis. Two mutated PLD analogues that were secreted to a level of 40% compared to the wild-type and retained minimal activity showed promise for development as recombinant CLA vaccines. Further work will be required to establish their suitability for commercialization.
Palavras-Chave: | Base Sequence; Expressão genética; Fosfolipase; Genetic toxoid; Inativacao gene; Molecular Sequence Data.
Thesagro: | Linfadenite Caseosa; Toxina; Vacina.
Thesaurus Nal: | Bacterial vaccines; Caseous lymphadenitis; Formaldehyde; Gene expression; Genetic resistance; Immunology; Phospholipase D; Sheep; Toxins.
Categoria do assunto: | --
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (10259-1) - ADD - AP - DD -  -  -  - DIS