Data/hora: 16/06/2024 10:27:31

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 25/04/1994
Data da última atualização: | 25/04/1994
Autoria: | CABRAL, G. B.; ARAGAO, F. J. L.; MONTE-NESHICH, D.C.; RECH, E. L.
Afiliação: | CERNARGEN-EMBRAPA.
Título: | Transient gene expression in cassava protoplasts .
Ano de publicação: | 1993
Fonte/Imprenta: | Cali, Colombia: CIAT, 1993
Páginas: | 244-250p
Série: | CIAT, Wording Document, 123
Idioma: | Inglês
Notas: | Cali, Colombia: CIAT, 1993.
Conteúdo: | The aim of the project is the development and improvement of methodologies for gene tranfer in cassava. Isoloted protoplasts provide a convenient system for studying the paramenters influencing the transformation of protoplasts by firect DNA uptake. Transient expression studies have utilized a plasmid  pAL carrying the beta-glucoronidase gene under control of 35S-CaMV promoter and nos polyadenilation region, following electrporation-mediated plasmid uptake. Protoplasts were isolated from cassave leaves (var. MCol22) growing in vitro, resluspended iln elevtroporation buffer (20 mM MES, pH 5.8, 10 mM CaCI2) and 20 mug ml-1 plasmid. A viability culrve of the optimum electroporation parameter gave an electric field of 715 V.cm-1 and a tilme constant =12.8 msec.   The expression of the gene product was analyzed by a qualitative and quantitative beta-glucuronidase enzyme assay. The results obtained from the foundation to study direct gene transfer in cassava. In addition, may allow a rapid method to evaluate the functional expression of tissue-specific promoters. Nevertheless, experiments have been carried out utilizing particle bombardment using tissues with regeneration capability. The production of transgenic plants shoud facilitate, in the future, studies to the introduction and expression of important agronomically traits in cassava.
Palavras-Chave: | Congresso; Maninhot esculenta; Producao: Alimentacao.
Thesagro: | Melhoramento.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (13440-1) - ADD - PL - -- - 633.6820601 - I61p - 1993000070 - DIS