Data/hora: 23/06/2024 13:03:52

Biblioteca(s): | Embrapa Recursos Genéticos e Biotecnologia.
Data corrente: | 07/06/2005
Data da última atualização: | 15/10/2007
Autoria: | STERKY, F.; MONTE, D. C.; SOMERVILLE, S.
Título: | Document 1/2 : development of a SAGEmap of Arabidopsis thaliana.
Ano de publicação: | 2001
Fonte/Imprenta: | In: MONTE, D. de C. Desenvolvendo estudos bioquímicos e moleculares do gene Mi1, que confere resistência a nematóides da galhas (Meloidogyne). Davis, CA: Universidade da Califórnia, Center for Engineering Resistance Against Pathogens; Stanford, CA: Carnegie Institution of Washington, 1999-2001.
Páginas: | Não paginado.
Idioma: | Inglês
Conteúdo: | SAGE (Serial Analysis of Gene Expression) was fjrst described by Velculescu et al. (1995) .It is a high throughput approach to study differential gene expression using the principie of restriction digestion of cDNAs, capture of the 3'region, a second difestion with a class II enzyme, ligation and concatemerization of tags, and subsequent highthroughput sequencencing of the tags, as indicated in Figures 1 and 2. The differential expression between a control and a reference sample (ex. Treated vs untreated, diseased VS. healthytissue, transgenic VS. wild type) is given by the ratio of the numbers of tags sequenced in both samples. SAGE technology has been very much used to study in animal research but not in plant research. One of the diffjculties to using SAGE technology is the need to have well defined genome sequences, specially the 3region of expressed sequences. The large number of sequence information available on EST's of Arabidopsis thaliana and the full completion of its genome sequence, made it a good model for construction of a SAGEmap. In the past, SAGE was considered cumbersome due to many steps in the tag cloning procedures. Recently, with the increase number of sequence information for the various organisms and the availability of SAGE technology kits through some biotech companies. SAGE has become much more popular. We expect that a SAGEmap for Arabidopsis will call the attention of the plant biologists to this high throughput approach to studying differential gene expression, that seems to suit well to laboratories already equipped to do highthroughput sequencing. not requiring expensive equipments as it is the case of the microarray technology. Here we report the development of the fjrst SAGEmap of a plant. At the moment, the map was developed based oa the utmost 3'Nla III site, based on TIGR annotation, April 2001, when 26819 features (EST sequences) were available. However, only 13% of these features had 3 'sequence information, as most sequences were 5 'derived and only 10% of the sequences had 5'untranslated region information (see Table 1). This map will be updated as soon as TIGR closes the full annotation of the genome. The Arabidopsis thaliana SAGEmap will soon e available throught The Arabidopsis Information Resources:TAIR ( www.arabidopsis.org ) and Arabidopsis Functional Genomics Consortium: AFGC (afgc.Stanford.edu) sites.
Palavras-Chave: | Expressão gênica diferencial; SAGEmap; Serial Analysis of Gene Expression.
Thesaurus Nal: | Arabidopsis thaliana.
Categoria do assunto: | --
Registro original: | Embrapa Recursos Genéticos e Biotecnologia (CENARGEN)
Exemplares: | CENARGEN - (25056-1) - UPC - PL - -- - 572.86 - M772d - 2005.0075a - DIS