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| 7. |  | CARREEL, F.; LEON, D. G. de; LAGODA, P.; LANAUD, C.; JENNY, C.; HORRY, J. P.; MONTCEL, H. T. du. Ascertaining maternal and paternal lineage within Musa by chloroplast and mitochondrial DNA RFLP analyses. Genome, v. 45, p. 679-692, 2002.| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 8. | | ESCALANT, J. V.; COTE, F.; GRAPPIN, A.; AUBOIRON, E.; LEGAVRE, T.; LAGODA, P.; CAREEL, F.; BAKRY, F.; HORRY, J. P.; KERBELEC, F.; TEISSON, C.; MONTCEL, H. T. du. Biotechnologies for Musa improvement - strategies and results Montpellier, France: INIBAP, 1994 p.22-26 In: Meeting of the Musa Breedrs Network, 1, 1994, La Lima, Honduras.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
16/06/2020 |
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Data da última atualização: |
16/06/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
NOGUEIRA, J. A. P.; FIGUEIREDO, A.; DUARTE, J. L.; ALMEIDA, F. B. de; SANTOS, M. G.; NASCIMENTO, L. M.; FERNANDES, C. P.; MOURÃO, S. C.; TOSCANO, J. H. B.; ROCHA, L. M.; CHAGAS, A. C. de S. |
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Afiliação: |
Jeane A. P. Nogueira, UFRJ; Amanda Figueiredo, UNESP; Jonatas L. Duarte, UNIFAP; Fernanda B. de Almeida, UNIFAP; Marcelo G. Santos, UERJ; Leonor M. Nascimento, UFRJ; Caio P. Fernandes, UNIFAP; Samanta C. Mourão, UFF; João Henrique B. Toscano, UNESP; Leandro M. Rocha, UFRJ; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
Repellency effect of Pilocarpus spicatus A. St.-Hil essential oil and nanoemulsion against Rhipicephalus microplus larvae. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Experimental Parasitology, 215, 107919, aug. 2020. |
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Páginas: |
5 p. |
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DOI: |
https://doi.org/10.1016/j.exppara.2020.107919 |
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Idioma: |
Inglês |
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Conteúdo: |
Rhipicephalus microplus, the cattle tick, is a major cause of economic losses in bovine production. Due to the widespread acaricidal resistance to commercially available products, as well as their toxicity and environmental impact, alternative control methods are required. Nanoformulations produced from plant extracts as bioactive substances are very promising as innovative acaricidal agents. Thus, the aim of this study was to evaluate the in vitro repellent activity of Pilocarpus spicatus essential oil and its nanoemulsion against R. microplus, using larval repellent test (RT). The essential oil was extracted by hydrodistillation, using a Clevenger-type apparatus. The nanoemulsion was prepared with 5% essential oil, 5% tween 80, and 90% water, using the phase inversion method (50 mg/mL). Limonene was the major component (46.8%) of the essential oil, as determined by gas chromatography-mass spectrometry (GC/MS) and confirmed by flame ionization detection (GC/FID). According to the RT results, the essential oil had a repellent activity greater than 69%, from concentrations of 3.12 mg/mL (69.81 ± 10%) to 50 mg/mL (98.10 ± 0.6%), whereas the nanoemulsion at 50 mg/mL presented repellent activities of 97.14 ± 1.37% and 97.89 ± 0.52% 6 and 10 h after treatment, respectively. These values regarding to total repellency were very close to those calculated for mortality corrected by Abbott's formula. The phase inversion method preserved the chemical and physical characteristics of the essential oil since both reached an equal repellent effect at the same concentration. Therefore, P. spicatus essential oil and nanoemulsion had excellent repellent activities against R. microplus larvae, demonstrating its potential for future use as an alternative for tick control. MenosRhipicephalus microplus, the cattle tick, is a major cause of economic losses in bovine production. Due to the widespread acaricidal resistance to commercially available products, as well as their toxicity and environmental impact, alternative control methods are required. Nanoformulations produced from plant extracts as bioactive substances are very promising as innovative acaricidal agents. Thus, the aim of this study was to evaluate the in vitro repellent activity of Pilocarpus spicatus essential oil and its nanoemulsion against R. microplus, using larval repellent test (RT). The essential oil was extracted by hydrodistillation, using a Clevenger-type apparatus. The nanoemulsion was prepared with 5% essential oil, 5% tween 80, and 90% water, using the phase inversion method (50 mg/mL). Limonene was the major component (46.8%) of the essential oil, as determined by gas chromatography-mass spectrometry (GC/MS) and confirmed by flame ionization detection (GC/FID). According to the RT results, the essential oil had a repellent activity greater than 69%, from concentrations of 3.12 mg/mL (69.81 ± 10%) to 50 mg/mL (98.10 ± 0.6%), whereas the nanoemulsion at 50 mg/mL presented repellent activities of 97.14 ± 1.37% and 97.89 ± 0.52% 6 and 10 h after treatment, respectively. These values regarding to total repellency were very close to those calculated for mortality corrected by Abbott's formula. The phase inversion method preserved the chemical and physical characteristics of the... Mostrar Tudo |
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Palavras-Chave: |
Cattle tick; Eco friendly acaricide; O/W nanoemulsion. |
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Thesaurus NAL: |
Livestock. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02746naa a2200313 a 4500
001 2123303
005 2020-06-16
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.exppara.2020.107919$2DOI
100 1 $aNOGUEIRA, J. A. P.
245 $aRepellency effect of Pilocarpus spicatus A. St.-Hil essential oil and nanoemulsion against Rhipicephalus microplus larvae.$h[electronic resource]
260 $c2020
300 $a5 p.
520 $aRhipicephalus microplus, the cattle tick, is a major cause of economic losses in bovine production. Due to the widespread acaricidal resistance to commercially available products, as well as their toxicity and environmental impact, alternative control methods are required. Nanoformulations produced from plant extracts as bioactive substances are very promising as innovative acaricidal agents. Thus, the aim of this study was to evaluate the in vitro repellent activity of Pilocarpus spicatus essential oil and its nanoemulsion against R. microplus, using larval repellent test (RT). The essential oil was extracted by hydrodistillation, using a Clevenger-type apparatus. The nanoemulsion was prepared with 5% essential oil, 5% tween 80, and 90% water, using the phase inversion method (50 mg/mL). Limonene was the major component (46.8%) of the essential oil, as determined by gas chromatography-mass spectrometry (GC/MS) and confirmed by flame ionization detection (GC/FID). According to the RT results, the essential oil had a repellent activity greater than 69%, from concentrations of 3.12 mg/mL (69.81 ± 10%) to 50 mg/mL (98.10 ± 0.6%), whereas the nanoemulsion at 50 mg/mL presented repellent activities of 97.14 ± 1.37% and 97.89 ± 0.52% 6 and 10 h after treatment, respectively. These values regarding to total repellency were very close to those calculated for mortality corrected by Abbott's formula. The phase inversion method preserved the chemical and physical characteristics of the essential oil since both reached an equal repellent effect at the same concentration. Therefore, P. spicatus essential oil and nanoemulsion had excellent repellent activities against R. microplus larvae, demonstrating its potential for future use as an alternative for tick control.
650 $aLivestock
653 $aCattle tick
653 $aEco friendly acaricide
653 $aO/W nanoemulsion
700 1 $aFIGUEIREDO, A.
700 1 $aDUARTE, J. L.
700 1 $aALMEIDA, F. B. de
700 1 $aSANTOS, M. G.
700 1 $aNASCIMENTO, L. M.
700 1 $aFERNANDES, C. P.
700 1 $aMOURÃO, S. C.
700 1 $aTOSCANO, J. H. B.
700 1 $aROCHA, L. M.
700 1 $aCHAGAS, A. C. de S.
773 $tExperimental Parasitology, 215, 107919, aug. 2020.
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