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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
30/10/2020 |
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Data da última atualização: |
30/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVEIRA, D. D.; BELLI FILHO, P.; PHILIPPI, L. S.; CANTAO, M. E.; FOULQUIER, A.; BAYLE, S.; DELFORNO, T. P.; MOLLE, P. |
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Afiliação: |
UFSC; PAULO BELLI FILHO, UFSC; UFSC; MAURICIO EGIDIO CANTAO, CNPSA; Univ. Savoie Mont Blanc Grenoble; Univ. Montpellier; UNICAMP; INRAE. |
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Título: |
In-depth assessment of microbial communities in the full-scale vertical flow treatment wetlands fed with raw domestic wastewater. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Environmental Technology, 09 Feb 2020 |
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DOI: |
https://doi.org/10.1080/09593330.2020.1723709 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT A multiphase study was proposed to examine microbial communities linked to the nitrogen cycle in the first stage of four full-scale French vertical flow treatment systems. To this end, denaturing gradient gel electrophoresis (DGGE) was performed for structural assessment and quantitative PCR (qPCR) to enumerate the abundance of ammonia-oxidizing (AOB). 16S rRNA sequencing was used to assess the taxonomic profile followed by putative assessment of functional genes. The samples were collected under different conditions, such as operational time (presence/absence of sludge layer on the surface of the filters), season (winter and summer), sampling depth (0, 15 and 30 cm) and operation cycle (rest and feed periods). A structural disparity was noted in the upper layers, whereas higher similarity at 30 cm was observed highlighting the effect of organic matter on bacterial diversity. The 7th rest day was highlighted by an apparent decline in the microbial community abundance. Additionally, qPCR indicated that the largest amount of AOB was found at 30 cm depth and during the feeding period. From the taxonomic profile, Mycobacterium, Acinetobacter, Flavobacterium, and Nitrospira were the most abundant genre found in all systems. The functional prediction results showed predicted genes linked to the denitrification process. The results suggested that operating time and season were responsible for the pattern of the microbial community behavior. This study allowed us to further understand the bacterial dynamics and to advance the idea of design modifications made in the first stage of the classical French system to improve nitrogen removal are promising. MenosABSTRACT A multiphase study was proposed to examine microbial communities linked to the nitrogen cycle in the first stage of four full-scale French vertical flow treatment systems. To this end, denaturing gradient gel electrophoresis (DGGE) was performed for structural assessment and quantitative PCR (qPCR) to enumerate the abundance of ammonia-oxidizing (AOB). 16S rRNA sequencing was used to assess the taxonomic profile followed by putative assessment of functional genes. The samples were collected under different conditions, such as operational time (presence/absence of sludge layer on the surface of the filters), season (winter and summer), sampling depth (0, 15 and 30 cm) and operation cycle (rest and feed periods). A structural disparity was noted in the upper layers, whereas higher similarity at 30 cm was observed highlighting the effect of organic matter on bacterial diversity. The 7th rest day was highlighted by an apparent decline in the microbial community abundance. Additionally, qPCR indicated that the largest amount of AOB was found at 30 cm depth and during the feeding period. From the taxonomic profile, Mycobacterium, Acinetobacter, Flavobacterium, and Nitrospira were the most abundant genre found in all systems. The functional prediction results showed predicted genes linked to the denitrification process. The results suggested that operating time and season were responsible for the pattern of the microbial community behavior. This study allowed us to further... Mostrar Tudo |
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Palavras-Chave: |
Comunidades microbianas; French VF wetland; Functional prediction; Microbial community structure; QPCR; Remoção de nitrogênio; Taxonomic profile. |
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Thesagro: |
Desnitrificação; Taxonomia. |
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Thesaurus Nal: |
Nitrifying bacteria. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02719naa a2200337 a 4500
001 2126167
005 2020-10-30
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1080/09593330.2020.1723709$2DOI
100 1 $aSILVEIRA, D. D.
245 $aIn-depth assessment of microbial communities in the full-scale vertical flow treatment wetlands fed with raw domestic wastewater.$h[electronic resource]
260 $c2020
520 $aABSTRACT A multiphase study was proposed to examine microbial communities linked to the nitrogen cycle in the first stage of four full-scale French vertical flow treatment systems. To this end, denaturing gradient gel electrophoresis (DGGE) was performed for structural assessment and quantitative PCR (qPCR) to enumerate the abundance of ammonia-oxidizing (AOB). 16S rRNA sequencing was used to assess the taxonomic profile followed by putative assessment of functional genes. The samples were collected under different conditions, such as operational time (presence/absence of sludge layer on the surface of the filters), season (winter and summer), sampling depth (0, 15 and 30 cm) and operation cycle (rest and feed periods). A structural disparity was noted in the upper layers, whereas higher similarity at 30 cm was observed highlighting the effect of organic matter on bacterial diversity. The 7th rest day was highlighted by an apparent decline in the microbial community abundance. Additionally, qPCR indicated that the largest amount of AOB was found at 30 cm depth and during the feeding period. From the taxonomic profile, Mycobacterium, Acinetobacter, Flavobacterium, and Nitrospira were the most abundant genre found in all systems. The functional prediction results showed predicted genes linked to the denitrification process. The results suggested that operating time and season were responsible for the pattern of the microbial community behavior. This study allowed us to further understand the bacterial dynamics and to advance the idea of design modifications made in the first stage of the classical French system to improve nitrogen removal are promising.
650 $aNitrifying bacteria
650 $aDesnitrificação
650 $aTaxonomia
653 $aComunidades microbianas
653 $aFrench VF wetland
653 $aFunctional prediction
653 $aMicrobial community structure
653 $aQPCR
653 $aRemoção de nitrogênio
653 $aTaxonomic profile
700 1 $aBELLI FILHO, P.
700 1 $aPHILIPPI, L. S.
700 1 $aCANTAO, M. E.
700 1 $aFOULQUIER, A.
700 1 $aBAYLE, S.
700 1 $aDELFORNO, T. P.
700 1 $aMOLLE, P.
773 $tEnvironmental Technology, 09 Feb 2020
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