| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Corte. |
|
Data corrente: |
22/01/2008 |
|
Data da última atualização: |
15/08/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
MIYOSHI, A.; ROSINHA, G. M. S.; CAMARGO I. L. B. C.; TRANT, C. M. C.; CARDOSO, F. C.; AZEVEDO, V.; OLIVEIRA, S. C. |
|
Afiliação: |
ANDERSON MIYOSHI, UNIVERSIDADE FEDERAL DE MINAS GERAIS; GRACIA MARIA SOARES ROSINHA, CNPGC; ILANA L. B. C. CAMARGO, UNIVERSIDADE FEDERAL DE MINAS GERAIS; CYNTIA M. C.TRANT, UNIVERSIDADE FEDERAL DE MINAS GERAIS; FERNANDA C. CARDOSO, UNIVERSIDADE FEDERAL DE MINAS GERAIS; VASCO AZEVEDO, UNIVERSIDADE FEDERAL DE MINAS GERAIS; SERGIO C. OLIVEIRA, UNIVERSIDADE FEDERAL DE MINAS GERAIS. |
|
Título: |
The role of the vacB gene in the pathogenesis of Brucella abortus. |
|
Ano de publicação: |
2007 |
|
Fonte/Imprenta: |
Microbes and infections, v. 9, n. 3, p. 375-381, Mar. 2007. |
|
DOI: |
https://doi.org/10.1016/j.micinf.2006.12.004 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Brucella species are important zoonotic pathogens affecting a wide variety of mammals. Therefore, the identification of new Brucella virulence factors is of great interest in understanding bacterial pathogenesis and immune evasion. In this study, we have identified Brucella abortus vacB gene that presents 2343 nucleotides and 781 amino acids and it shows 39% identity with Shigella flexneri vacB gene that encodes an exoribonuclease RNase R involved in bacterial virulence. Further, we have inactivated Brucella vacB by gene replacement strategy generating a deletion mutant strain. In order to test the role of Brucella vacB in pathogenesis, BALB/c and interferon regulatory factor-1 (IRF-1) knockout (KO) mice received Brucella vacB mutant, the virulent parental strain 2308 or the vaccine strain RB51 and the bacterial CFU numbers in spleens and mous survival were monitored. Our results demonstrated that the B. abortus DvacB mutant and the wild type strain 2308 showed similar CFU numbers in BALB/c mice. Additionally, IRF-1 KO mice that received either the vacB mutant or S2308 strain died in 12e14 days postinfection; in contrast, all animals that received the RB51 vaccine strain survived for 30 days postinoculation. In summary, this study reports that the vacB gene in B. abortus has no impact on bacterial pathogenesis. |
|
Thesagro: |
Bovino; Brucella Abortus; Brucelose; Sanidade Animal. |
|
Thesaurus Nal: |
Animal health; Cattle; Pathogenesis. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02134naa a2200289 a 4500
001 1324217
005 2023-08-15
008 2007 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.micinf.2006.12.004$2DOI
100 1 $aMIYOSHI, A.
245 $aThe role of the vacB gene in the pathogenesis of Brucella abortus.
260 $c2007
520 $aBrucella species are important zoonotic pathogens affecting a wide variety of mammals. Therefore, the identification of new Brucella virulence factors is of great interest in understanding bacterial pathogenesis and immune evasion. In this study, we have identified Brucella abortus vacB gene that presents 2343 nucleotides and 781 amino acids and it shows 39% identity with Shigella flexneri vacB gene that encodes an exoribonuclease RNase R involved in bacterial virulence. Further, we have inactivated Brucella vacB by gene replacement strategy generating a deletion mutant strain. In order to test the role of Brucella vacB in pathogenesis, BALB/c and interferon regulatory factor-1 (IRF-1) knockout (KO) mice received Brucella vacB mutant, the virulent parental strain 2308 or the vaccine strain RB51 and the bacterial CFU numbers in spleens and mous survival were monitored. Our results demonstrated that the B. abortus DvacB mutant and the wild type strain 2308 showed similar CFU numbers in BALB/c mice. Additionally, IRF-1 KO mice that received either the vacB mutant or S2308 strain died in 12e14 days postinfection; in contrast, all animals that received the RB51 vaccine strain survived for 30 days postinoculation. In summary, this study reports that the vacB gene in B. abortus has no impact on bacterial pathogenesis.
650 $aAnimal health
650 $aCattle
650 $aPathogenesis
650 $aBovino
650 $aBrucella Abortus
650 $aBrucelose
650 $aSanidade Animal
700 1 $aROSINHA, G. M. S.
700 1 $aCAMARGO I. L. B. C.
700 1 $aTRANT, C. M. C.
700 1 $aCARDOSO, F. C.
700 1 $aAZEVEDO, V.
700 1 $aOLIVEIRA, S. C.
773 $tMicrobes and infections$gv. 9, n. 3, p. 375-381, Mar. 2007.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Corte (CNPGC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Agroenergia. |
|
Data corrente: |
19/10/2012 |
|
Data da última atualização: |
21/09/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
FAVARO, L. C. de L.; SEBASTIANES, F. L. DE S.; ARAUJO, W. L. |
|
Afiliação: |
LEIA CECILIA DE LIMA FAVARO, CNPAE; Fernanda Luiza de Souza Sebastianes, USP; Welington Luiz Araujo, USP. |
|
Título: |
Epicoccum nigrumP16, a Sugarcane Endophyte, Produces Antifungal Compounds and Induces Root Growth. |
|
Ano de publicação: |
2012 |
|
Fonte/Imprenta: |
PLoS ONE, v. 7, n. 6, e36826, p. 1-10, 2012. |
|
DOI: |
10.1371/journal.pone.0036826 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Background:Sugarcane is one of the most important crops in Brazil, mainly because of its use in biofuel production. Recent studies have sought to determine the role of sugarcane endophytic microbial diversity in microorganism-plant interactions, and their biotechnological potential. Epicoccum nigrum is an important sugarcane endophytic fungus that has been associated with the biological control of phytopathogens, and the production of secondary metabolites. In spite of several studies carried out to define the better conditions to useE. nigrumin different crops, little is known about the establishment of an endophytic interaction, and its potential effects on plant physiology. Methodology/Principal Findings:We report an approach based on inoculation followed by re-isolation, molecular monitoring, microscopic analysis, plant growth responses to fungal colonization, and antimicrobial activity tests to study the basic aspects of the E. nigrum endophytic interaction with sugarcane, and the effects of colonization on plant physiology. The results indicate thatE. nigrum was capable of increasing the root system biomass and producing compounds that inhibit the in vitro growth of sugarcane pathogensFusarium verticillioides, Colletotrichum falcatum, Ceratocystis paradoxa, and Xanthomomas albilineans. In addition, E. nigrumpreferentially colonizes the sugarcane surface and, occasionally, the endophytic environment. Conclusions/Significance:Our work demonstrates thatE. nigrum has great potential for sugarcane crop application because it is capable of increasing the root system biomass and controlling pathogens. The study of the basic aspects of the interaction of E. nigrumwith sugarcane demonstrated the facultative endophytism ofE. nigrumand its preference for the phylloplane environment, which should be considered in future studies of biocontrol using this species. In addition, this work contributes to the knowledge of the interaction of this ubiquitous endophyte with the host plant, and also to a better use of microbial endophytes in agriculture. MenosBackground:Sugarcane is one of the most important crops in Brazil, mainly because of its use in biofuel production. Recent studies have sought to determine the role of sugarcane endophytic microbial diversity in microorganism-plant interactions, and their biotechnological potential. Epicoccum nigrum is an important sugarcane endophytic fungus that has been associated with the biological control of phytopathogens, and the production of secondary metabolites. In spite of several studies carried out to define the better conditions to useE. nigrumin different crops, little is known about the establishment of an endophytic interaction, and its potential effects on plant physiology. Methodology/Principal Findings:We report an approach based on inoculation followed by re-isolation, molecular monitoring, microscopic analysis, plant growth responses to fungal colonization, and antimicrobial activity tests to study the basic aspects of the E. nigrum endophytic interaction with sugarcane, and the effects of colonization on plant physiology. The results indicate thatE. nigrum was capable of increasing the root system biomass and producing compounds that inhibit the in vitro growth of sugarcane pathogensFusarium verticillioides, Colletotrichum falcatum, Ceratocystis paradoxa, and Xanthomomas albilineans. In addition, E. nigrumpreferentially colonizes the sugarcane surface and, occasionally, the endophytic environment. Conclusions/Significance:Our work demonstrates thatE. nigrum has great... Mostrar Tudo |
|
Thesaurus NAL: |
sugarcane. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02615naa a2200169 a 4500
001 1937252
005 2017-09-21
008 2012 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0036826$2DOI
100 1 $aFAVARO, L. C. de L.
245 $aEpicoccum nigrumP16, a Sugarcane Endophyte, Produces Antifungal Compounds and Induces Root Growth.$h[electronic resource]
260 $c2012
520 $aBackground:Sugarcane is one of the most important crops in Brazil, mainly because of its use in biofuel production. Recent studies have sought to determine the role of sugarcane endophytic microbial diversity in microorganism-plant interactions, and their biotechnological potential. Epicoccum nigrum is an important sugarcane endophytic fungus that has been associated with the biological control of phytopathogens, and the production of secondary metabolites. In spite of several studies carried out to define the better conditions to useE. nigrumin different crops, little is known about the establishment of an endophytic interaction, and its potential effects on plant physiology. Methodology/Principal Findings:We report an approach based on inoculation followed by re-isolation, molecular monitoring, microscopic analysis, plant growth responses to fungal colonization, and antimicrobial activity tests to study the basic aspects of the E. nigrum endophytic interaction with sugarcane, and the effects of colonization on plant physiology. The results indicate thatE. nigrum was capable of increasing the root system biomass and producing compounds that inhibit the in vitro growth of sugarcane pathogensFusarium verticillioides, Colletotrichum falcatum, Ceratocystis paradoxa, and Xanthomomas albilineans. In addition, E. nigrumpreferentially colonizes the sugarcane surface and, occasionally, the endophytic environment. Conclusions/Significance:Our work demonstrates thatE. nigrum has great potential for sugarcane crop application because it is capable of increasing the root system biomass and controlling pathogens. The study of the basic aspects of the interaction of E. nigrumwith sugarcane demonstrated the facultative endophytism ofE. nigrumand its preference for the phylloplane environment, which should be considered in future studies of biocontrol using this species. In addition, this work contributes to the knowledge of the interaction of this ubiquitous endophyte with the host plant, and also to a better use of microbial endophytes in agriculture.
650 $asugarcane
700 1 $aSEBASTIANES, F. L. DE S.
700 1 $aARAUJO, W. L.
773 $tPLoS ONE$gv. 7, n. 6, e36826, p. 1-10, 2012.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agroenergia (CNPAE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
