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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
08/02/2024 |
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Data da última atualização: |
08/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CUNHA, T.; MIRANDA, M. P.; ZANARDI, O. Z.; MONNERAT, R.; MARQUES, J. P. R.; DORTA, S. O.; MACEDO, C. L.; MACHADO, M. A.; ASTUA, J. de F. |
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Afiliação: |
TATIANE CUNHA, UNIVERSIDADE DE SÃO PAULO; MARCELO P. MIRANDA, FUNDO DE DEFESA DA CITRICULTURA (FUNDECITRUS); ODIMAR Z. ZANARDI, INSTITUTO FEDERAL DE SANTA CATARINA; ROSE MONNERAT, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA; JOÃO P.R. MARQUES, UNIVERSITY OF SÃO PAULO; SÍLVIA O. DORTA, UNIVERSIDADE DE SÃO PAULO; CRISTINA L. MACEDO, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA; MARCOS A. MACHADO, CENTRO DE CITRICULTURA SYLVIO MOREIRA; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
Bacillus thuringiensis translocation in citrus scion/rootstock combinations and binding of cry toxins with the Asian citrus psyllid gut receptors. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Journal Pre-proof, Crop Protection, 2024. |
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Páginas: |
46 p. |
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DOI: |
https://doi.org/10.1016/j.cropro.2024.106593 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Bacillus thuringiensis (Bt) can be effective in controlling insect pests either in spray products or by their toxins expressed in transgenic crops. The discovery of Bt as endophytic in citrus opened new perspectives for the control of Diaphorina citri Kuwayama, the vector of the bacteria Candidatus Liberibacter spp. associated with Huanglongbing. In this study, the endophytic translocation of Bt in citrus seedlings (small and large plants) and in different scion-rootstock combinations were investigated. We also assessed the lethal concentration of D. citri nymphs caused by systemic Bt in citrus and, moreover, the binding of Cry toxins with the Asian citrus psyllid (ACP) gut receptors. Endophytic translocation of Bt in citrus seedlings and nursery trees was confirmed by colony isolation, PCR detection, and Btk-GFP microscopy analyses, which demonstrated that, after Bt inoculation into the substrate, the bacteria enter through lateral root emergence sites and are localized in stem xylem vessel elements. Mean lethal concentrations for D. citri third instar nymphs were 4.92 × 104 and 2.19 × 104 spores mL−1 for S1450 and S1302 strains, respectively. Nymphal mortality induced by the bacteria was higher in citrus seedlings than in citrus nursery trees. All Cry-toxins tested were able to bind to D. citri brush border membrane vesicles, suggesting the potential pathogenicity of Bt strains against psyllid. This study clearly demonstrates the penetration and Bt translocation in the xylem of small and large citrus plants and the potential of the bacteria to cause ACP nymphs mortality that fed on shoots from Bt-treated citrus. MenosAbstract: Bacillus thuringiensis (Bt) can be effective in controlling insect pests either in spray products or by their toxins expressed in transgenic crops. The discovery of Bt as endophytic in citrus opened new perspectives for the control of Diaphorina citri Kuwayama, the vector of the bacteria Candidatus Liberibacter spp. associated with Huanglongbing. In this study, the endophytic translocation of Bt in citrus seedlings (small and large plants) and in different scion-rootstock combinations were investigated. We also assessed the lethal concentration of D. citri nymphs caused by systemic Bt in citrus and, moreover, the binding of Cry toxins with the Asian citrus psyllid (ACP) gut receptors. Endophytic translocation of Bt in citrus seedlings and nursery trees was confirmed by colony isolation, PCR detection, and Btk-GFP microscopy analyses, which demonstrated that, after Bt inoculation into the substrate, the bacteria enter through lateral root emergence sites and are localized in stem xylem vessel elements. Mean lethal concentrations for D. citri third instar nymphs were 4.92 × 104 and 2.19 × 104 spores mL−1 for S1450 and S1302 strains, respectively. Nymphal mortality induced by the bacteria was higher in citrus seedlings than in citrus nursery trees. All Cry-toxins tested were able to bind to D. citri brush border membrane vesicles, suggesting the potential pathogenicity of Bt strains against psyllid. This study clearly demonstrates the penetration and Bt translocation ... Mostrar Tudo |
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Thesagro: |
Porta Enxerto; Translocação. |
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Thesaurus Nal: |
Brush border membrane vesicles; Citrus; Diaphorina citri; Endotoxins. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02611naa a2200313 a 4500
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024 7 $ahttps://doi.org/10.1016/j.cropro.2024.106593$2DOI
100 1 $aCUNHA, T.
245 $aBacillus thuringiensis translocation in citrus scion/rootstock combinations and binding of cry toxins with the Asian citrus psyllid gut receptors.$h[electronic resource]
260 $c2024
300 $a46 p.
520 $aAbstract: Bacillus thuringiensis (Bt) can be effective in controlling insect pests either in spray products or by their toxins expressed in transgenic crops. The discovery of Bt as endophytic in citrus opened new perspectives for the control of Diaphorina citri Kuwayama, the vector of the bacteria Candidatus Liberibacter spp. associated with Huanglongbing. In this study, the endophytic translocation of Bt in citrus seedlings (small and large plants) and in different scion-rootstock combinations were investigated. We also assessed the lethal concentration of D. citri nymphs caused by systemic Bt in citrus and, moreover, the binding of Cry toxins with the Asian citrus psyllid (ACP) gut receptors. Endophytic translocation of Bt in citrus seedlings and nursery trees was confirmed by colony isolation, PCR detection, and Btk-GFP microscopy analyses, which demonstrated that, after Bt inoculation into the substrate, the bacteria enter through lateral root emergence sites and are localized in stem xylem vessel elements. Mean lethal concentrations for D. citri third instar nymphs were 4.92 × 104 and 2.19 × 104 spores mL−1 for S1450 and S1302 strains, respectively. Nymphal mortality induced by the bacteria was higher in citrus seedlings than in citrus nursery trees. All Cry-toxins tested were able to bind to D. citri brush border membrane vesicles, suggesting the potential pathogenicity of Bt strains against psyllid. This study clearly demonstrates the penetration and Bt translocation in the xylem of small and large citrus plants and the potential of the bacteria to cause ACP nymphs mortality that fed on shoots from Bt-treated citrus.
650 $aBrush border membrane vesicles
650 $aCitrus
650 $aDiaphorina citri
650 $aEndotoxins
650 $aPorta Enxerto
650 $aTranslocação
700 1 $aMIRANDA, M. P.
700 1 $aZANARDI, O. Z.
700 1 $aMONNERAT, R.
700 1 $aMARQUES, J. P. R.
700 1 $aDORTA, S. O.
700 1 $aMACEDO, C. L.
700 1 $aMACHADO, M. A.
700 1 $aASTUA, J. de F.
773 $tJournal Pre-proof, Crop Protection, 2024.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
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Tipo/Formato |
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Registro |
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| Registros recuperados : 17 | |
| 3. |  | ASSIS, D. P.; OLIVEIRA, I. S.; NASCIMENTO, A. S. do; MIRANDA, M. P. de; AZEVEDO FILHO, W. S. de. Atratividade de cigarrinhas de xilema (Hemiptera: Cicadellidae) pelo boldo, Vernonia condensata Becker, em pomar de laranja Pera. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 8., 2014, Cruz das Almas, Ba. Pesquisa: despertando mentes para a inovação e transformando o futuro : [anais]. Cruz das Almas, BA, Embrapa Mandioca e Fruticultura, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 4. | | GIRARDI, E. A.; BASSANEZI, R. B.; ANDRADE, E. C. de; MIRANDA, M. P. de; BARBOSA, F. F. L. Identificação e doença huanglongbing (HLB) Informe Agropecuário, Belo Horizonte, v. 35, n. 281, p. 64-76, jul./ago. 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 6. | | VOLPE, H. X. L.; FAZOLIN, M.; GARCIA, R. B.; MAGNANI, R. F.; BARBOSA, J. C.; MIRANDA, M. P. Efficacy of essential oil of Piper aduncum against nymphs and adults of Diaphorina citri. Pest Management Science, New Jersey, v. 72, n. 6, p. 1242-1249, June 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Acre. |
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| 8. | | MAMEDE, M. E. O.; MIRANDA, M. P. S.; RITZINGER, R.; GODOY, R. C. B. de; VELOZO, E. S. Physico-chemical and sensorial evaluation of new varieties of acerola. British Food Journal,United Kingdom, v. 111, n. 4, p. 387-395, 2009. DOI 10.1108/00070700910951867| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 9. | | ASSIS, D. P.; OLIVEIRA, I. S.; MIRANDA, M. P. de; AZEVEDO FILHO, W. S. de; MELO, R. L. de; BARBOSA, C. de J.; NASCIMENTO, A. S. do. Atratividade a cigarrinhas de xilema (Hemiptera: Cicadellidae) por plantas de boldo (Vernonia condensata Becker), em pomar de laranja 'Pera'. Cruz das Almas: Embrapa Mandioca e Fruticultura, 2019. (Embrapa Mandioca e Fruticultura. Boletim de Pesquisa e Desenvolvimento, 104).| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 10. | | CUNHA, T.; MIRANDA, M. P.; ZANARDI, O. Z.; MONNERAT, R.; MARQUES, J. P. R.; DORTA, S. O.; MACEDO, C. L.; MACHADO, M. A.; ASTUA, J. de F. Bacillus thuringiensis translocation in citrus scion/rootstock combinations and binding of cry toxins with the Asian citrus psyllid gut receptors. Journal Pre-proof, Crop Protection, 2024. 46 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 11. | | SILVA, J. B. N. de J.; CRUZ, M. A.; MELO, R. L. de; ABREU, E. F. M.; MIRANDA, M. P. de; NASCIMENTO, A. S. do. Levantamento e identificação de espécies de cigarrinhas de xilema, vetoras da CVC em pomares de citros no Recôncavo Sul da Bahia. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA TROPICAL, 7., 2013, Cruz das Almas. Anais... Cruz das Almas: Embrapa Mandioca e Fruticultura, 2013. Publicação online.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 14. | | ZANARDI, O. Z.; VOLPE, H. X. L.; FAZOLIN, M.; VEIGA, A. C. P.; FREITAS, R. de; CARVALHO, R. I.; MAGNANI, R. F.; MIRANDA, M. P. Toxicity of dillapiole-based essential oil to Diaphorina citri Kuwayama nymphs and Tamarixia radiata (Waterston) adults. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 27.; CONGRESSO LATINO-AMERICANO DE ENTOMOLOGIA, 10., 2018, Gramado. Entomologia: saúde, ambiente e agricultura: anais. Curitiba: SBE; Santa Maria: UFSM, 2018. p. 1130.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Acre. |
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| 15. | | DORTA, S. de O.; BALBINOTTE, J.; MONNERAT, R.; LOPES, J. R. S.; CUNHA, T. da; ZANARDI, O. Z.; MIRANDA, M. P. de; MACHADO, M. A.; ASTUA, J. de F. Selection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera: Liviidae) nymphs. Insect Science, p.1-12, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 16. | | DORTA, S. de O.; BALBINOTTE, J.; MONNERAT, R.; LOPES, J. R. S.; CUNHA, T. da; ZANARDI, O. Z.; MIRANDA, M. P. de; MACHADO, M. A.; ASTUA, J. de F. Selection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera: Liviidae) nymphs. Insect Science, v. 27, n. 3, p. 519-530, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | ALMEIDA, E. V. de; SCHNADELBACH, A. S.; NASCIMENTO, A. S. do; BARBOSA, C. de J.; ABREU, E. F. M.; BARBOSA, F. F. L.; SANTOS FILHO, H. P.; SILVA, I. P. da; REZENDE, J. de O.; J. M. C. de; BARBOSA, L. V.; MIRANDA, M. P. de; MIRANDA, S. H. G. de; SILVA, S. X. de B.; CASAIS, V. O.; AZEVEDO FILHO, W. S. de. Rede de Pesquisa para o Estudo do Patossistema da clorose variegada dos citros no Estado da Bahia: resultados de quatro anos de avaliações. Cruz das Almas, Ba: Embrapa Mandioca e Fruticultura. 2017. (Embrapa Mandioca e Fruticultura. Boletim de Pesquisa e Desenvolvimento, 85).| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 17 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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