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Registro Completo |
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Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
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Data corrente: |
31/01/2019 |
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Data da última atualização: |
14/02/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TORATI, L. S.; TAGGART, J. B.; VARELA, E. S.; ARARIPE, J.; WEHNER, S.; MIGAUD, H. |
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Afiliação: |
LUCAS SIMON TORATI, CNPASA; JOHN BERNARD TAGGART, UNIVERSITY OF STIRLING, Scotland-UK; EDUARDO SOUSA VARELA, CNPASA; JULIANA ARARIPE, UNIVERSIDADE FEDERAL DO PARÁ, Bragança-PA; STEFANIE WEHNER, MAX PLANCK INSTITUTE, Munich; HERVÉ MIGAUD, UNIVERSITY OF STIRLING, Scotland-UK. |
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Título: |
Genetic diversity and structure in Arapaima gigas populations from Amazon and Araguaia-Tocantins river basins. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
BMC Genetics, v. 20, n. 13, Jan. 2019. |
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ISSN: |
1471-2156 |
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DOI: |
10.1186/s12863-018-0711-y |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Arapaima gigas (Schinz, 1822) is the largest freshwater scaled fish in the world, and an emerging species for tropical aquaculture development. Conservation of the species, and the expansion of aquaculture requires the development of genetic tools to study polymorphism, differentiation, and stock structure. This study aimed to investigate genomic polymorphism through ddRAD sequencing, in order to identify a panel of single nucleotide polymorphisms (SNPs) and to simultaneously assess genetic diversity and structure in wild (from rivers Amazon, Solimões, Tocantins and Araguaia) and captive populations. Results: Compared to many other teleosts, the degree of polymorphism in A. gigas was low with only 2.3% of identified RAD-tags (135 bases long) containing SNPs. A panel of 393 informative SNPs was identified and screened across the five populations. Higher genetic diversity indices (number of polymorphic loci and private alleles, Shannon?s Index and HO) were found in populations from the Amazon and Solimões, intermediate levels in Tocantins and Captive, and very low levels in the Araguaia population. These results likely reflect larger population sizes from less urbanized environments in the Amazon basin compared to Araguaia. Populations were significantly differentiated with pairwise FST values ranging from 0.086 (Amazon × Solimões) to 0.556 (Amazon × Araguaia). Mean pairwise relatedness among individuals was significant in all populations (P < 0.01), reflecting a degree of inbreeding possibly due to severe depletion of natural stocks, the species sedentary behaviour and possible sampling biases. Although Mantel test was not significant (P = 0.104; R2 = 0.65), Bayesian analysis in STRUCTURE and discriminant analysis of principal components (DAPC) showed populations of Amazon and Solimões to be genetically differentiated from Araguaia, with Tocantins comprising individuals from both identified stocks. Conclusions: This relatively rapid genotyping by sequencing approach proved to be successful in delineating arapaima stocks. The approach and / or SNP panels identified should prove valuable for more detailed genetic studies of arapaima populations, including the elucidation of the genetic status of described discrete morphotypes and aid in delivery of conservation programs to maintain genetic diversity in reservoirs across the Amazon region. MenosBackground: Arapaima gigas (Schinz, 1822) is the largest freshwater scaled fish in the world, and an emerging species for tropical aquaculture development. Conservation of the species, and the expansion of aquaculture requires the development of genetic tools to study polymorphism, differentiation, and stock structure. This study aimed to investigate genomic polymorphism through ddRAD sequencing, in order to identify a panel of single nucleotide polymorphisms (SNPs) and to simultaneously assess genetic diversity and structure in wild (from rivers Amazon, Solimões, Tocantins and Araguaia) and captive populations. Results: Compared to many other teleosts, the degree of polymorphism in A. gigas was low with only 2.3% of identified RAD-tags (135 bases long) containing SNPs. A panel of 393 informative SNPs was identified and screened across the five populations. Higher genetic diversity indices (number of polymorphic loci and private alleles, Shannon?s Index and HO) were found in populations from the Amazon and Solimões, intermediate levels in Tocantins and Captive, and very low levels in the Araguaia population. These results likely reflect larger population sizes from less urbanized environments in the Amazon basin compared to Araguaia. Populations were significantly differentiated with pairwise FST values ranging from 0.086 (Amazon × Solimões) to 0.556 (Amazon × Araguaia). Mean pairwise relatedness among individuals was significant in all populations (P < 0.01), reflecting a d... Mostrar Tudo |
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Palavras-Chave: |
Araguaia; Tocantins. |
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Thesagro: |
Arapauma Gigas; Conservação; Genética Animal; Pirarucu. |
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Thesaurus Nal: |
Amazonia; Aquaculture; Arapaima gigas; Fisheries; Rivers; Species diversity. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/191822/1/CNPASA-2019-bmcgenetics.pdf
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Marc: |
LEADER 03345naa a2200349 a 4500
001 2105220
005 2020-02-14
008 2019 bl uuuu u00u1 u #d
022 $a1471-2156
024 7 $a10.1186/s12863-018-0711-y$2DOI
100 1 $aTORATI, L. S.
245 $aGenetic diversity and structure in Arapaima gigas populations from Amazon and Araguaia-Tocantins river basins.$h[electronic resource]
260 $c2019
520 $aBackground: Arapaima gigas (Schinz, 1822) is the largest freshwater scaled fish in the world, and an emerging species for tropical aquaculture development. Conservation of the species, and the expansion of aquaculture requires the development of genetic tools to study polymorphism, differentiation, and stock structure. This study aimed to investigate genomic polymorphism through ddRAD sequencing, in order to identify a panel of single nucleotide polymorphisms (SNPs) and to simultaneously assess genetic diversity and structure in wild (from rivers Amazon, Solimões, Tocantins and Araguaia) and captive populations. Results: Compared to many other teleosts, the degree of polymorphism in A. gigas was low with only 2.3% of identified RAD-tags (135 bases long) containing SNPs. A panel of 393 informative SNPs was identified and screened across the five populations. Higher genetic diversity indices (number of polymorphic loci and private alleles, Shannon?s Index and HO) were found in populations from the Amazon and Solimões, intermediate levels in Tocantins and Captive, and very low levels in the Araguaia population. These results likely reflect larger population sizes from less urbanized environments in the Amazon basin compared to Araguaia. Populations were significantly differentiated with pairwise FST values ranging from 0.086 (Amazon × Solimões) to 0.556 (Amazon × Araguaia). Mean pairwise relatedness among individuals was significant in all populations (P < 0.01), reflecting a degree of inbreeding possibly due to severe depletion of natural stocks, the species sedentary behaviour and possible sampling biases. Although Mantel test was not significant (P = 0.104; R2 = 0.65), Bayesian analysis in STRUCTURE and discriminant analysis of principal components (DAPC) showed populations of Amazon and Solimões to be genetically differentiated from Araguaia, with Tocantins comprising individuals from both identified stocks. Conclusions: This relatively rapid genotyping by sequencing approach proved to be successful in delineating arapaima stocks. The approach and / or SNP panels identified should prove valuable for more detailed genetic studies of arapaima populations, including the elucidation of the genetic status of described discrete morphotypes and aid in delivery of conservation programs to maintain genetic diversity in reservoirs across the Amazon region.
650 $aAmazonia
650 $aAquaculture
650 $aArapaima gigas
650 $aFisheries
650 $aRivers
650 $aSpecies diversity
650 $aArapauma Gigas
650 $aConservação
650 $aGenética Animal
650 $aPirarucu
653 $aAraguaia
653 $aTocantins
700 1 $aTAGGART, J. B.
700 1 $aVARELA, E. S.
700 1 $aARARIPE, J.
700 1 $aWEHNER, S.
700 1 $aMIGAUD, H.
773 $tBMC Genetics$gv. 20, n. 13, Jan. 2019.
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Registro original: |
Embrapa Pesca e Aquicultura (CNPASA) |
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| Registros recuperados : 12 | |
| 10. |  | TORATI, L. S.; LIMA, A. F.; MESQUITA, P. E. C.; TAGGART, J. B.; ALBALAT, A.; TAYLOR, J.; MIGAUD, H. Advances into the captive reproduction of the amazon pirarucu (Osteoglossidae: Arapaima). In: FENACAM & LACQUA/SARA (WAS)'15.; LATIN AMERICAN & CARIBBEAN AQUACULTURE 15.; SOUTH AMERICAN REGIONAL AQUACULTURE 15.; INTERNATIONAL SHRIMP FARMING SYMPOSIUM, 12.; INTERNATIONAL ACQUACULTURE TRADE SHOW, 12.; INTERNATIONAL AQUACULTURE SYMPOSIUM, 9.; TILAPIA ECONOMIC FORUM, 3., 2015, Fortaleza. Abstracts... Fortaleza: ABCC: World Aquaculture Society, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pesca e Aquicultura. |
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| 11. | | TORATI, L. S.; MIGAUD, H.; DOHERTY, M. K.; SIWY, J.; MULLEN, W.; MESQUITA, P. E. C.; ALBALAT, A. Comparative proteome and peptidome analysis of the cephalic fluid secreted by Arapaima gigas (Teleostei: Osteoglossidae) during and outside parental care. Plos One, v. 12, n. 10, e0186692, Oct. 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pesca e Aquicultura. |
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| 12. | | VARELA, E. S.; BEKAERT, M.; KIRSCHNIK, L. N. G.; TORATI, L. S.; SHIOTSUKI, L.; O'SULLIVAN, F. L. A.; VILLELA, L. C. V.; REZENDE, F. P.; BARROSO, A. da S.; FREITAS, L. E. L. de; TAGGART, J. B.; MIGAUD, H. A high-density linkage map and sex-linked markers for the Amazon tambaqui Colossoma macropomum. BMC Genomics, v. 22, n. 709, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Pesca e Aquicultura. |
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| Registros recuperados : 12 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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