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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
02/06/2000 |
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Data da última atualização: |
07/03/2013 |
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Autoria: |
MEZENCIO, J. M. S.; MOUSSATCHE, N.; MOSCARDI, F.; REBELLO, M. A. |
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Título: |
DNA-dependent RNA polymerase activity associated with Anticarsia gemmatalis nuclear polyhedrosis virus. |
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Ano de publicação: |
1986 |
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Fonte/Imprenta: |
In: ENCONTRO NACIONAL DE VIROLOGIA, 3., 1986, Sao Lourenco. Resumos... Rio de Janeiro: Centro Panamericano de Febre Aftosa, 1986. p.103. |
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Idioma: |
Inglês |
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Conteúdo: |
Anticarsia gemmatalis nuclear polyhedrosis virus (Ag-NPV) is an insect patogen, member of the Baculoviridae family. This group of virus contains a large, circular double-strand DNA genome at 7,5-10 x 10 daltons, that replicates in the nuclei of infected host cells. The infection leads to the formation of polyhedral inclusion bodies referred polyhedra (Summers & Smith, Virology, 84:390-402, 1978). Studies on the replication of baculovirus has been demonstrated the induction of DNA-dependent DNA polymerase (Miller et al., J. Virol., 40:305-308, 1981) and alpha-amanitin resistant DNA-dependent RNA polymerase (Fucns et al., J. Virol., 48:641-646, 1983) by Autographa californica nuclear polyedrosis virus in lepidoptera infected cells. This results suggest the presence of a viral RNA polymerase codified by the viral genone. In this communication we report a possible DNA-dependent RNA polymerase associated with Ag-NPV polyhedra. The enzyme activity was measured after activation the mixture contained polyhedra with sarkosyl or alpha-chymotrypsin. The enzyme was resistant to alpha-amanitin, and the addition of rifampicin, actinomycin D. ethidium bromide or neutral acriflavin, inhibit the transcription activity to some extent. The transcription products analysed by sucrose gradient centrifugation shows the presence of 3 RNA peaks. This results suggest a possible presence of RNA polymerase associated with Ag-NPV polyhedra. |
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Palavras-Chave: |
Anticarsia germmatalis; Brasil; Insects pest; Insetos pragas; Nuclear polyhedrosis virus; Poliedrose nuclear. |
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Thesagro: |
Controle Biológico; Vírus. |
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Thesaurus Nal: |
biological control; Brazil. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02312naa a2200277 a 4500
001 1461949
005 2013-03-07
008 1986 bl --- 0-- u #d
100 1 $aMEZENCIO, J. M. S.
245 $aDNA-dependent RNA polymerase activity associated with Anticarsia gemmatalis nuclear polyhedrosis virus.
260 $c1986
520 $aAnticarsia gemmatalis nuclear polyhedrosis virus (Ag-NPV) is an insect patogen, member of the Baculoviridae family. This group of virus contains a large, circular double-strand DNA genome at 7,5-10 x 10 daltons, that replicates in the nuclei of infected host cells. The infection leads to the formation of polyhedral inclusion bodies referred polyhedra (Summers & Smith, Virology, 84:390-402, 1978). Studies on the replication of baculovirus has been demonstrated the induction of DNA-dependent DNA polymerase (Miller et al., J. Virol., 40:305-308, 1981) and alpha-amanitin resistant DNA-dependent RNA polymerase (Fucns et al., J. Virol., 48:641-646, 1983) by Autographa californica nuclear polyedrosis virus in lepidoptera infected cells. This results suggest the presence of a viral RNA polymerase codified by the viral genone. In this communication we report a possible DNA-dependent RNA polymerase associated with Ag-NPV polyhedra. The enzyme activity was measured after activation the mixture contained polyhedra with sarkosyl or alpha-chymotrypsin. The enzyme was resistant to alpha-amanitin, and the addition of rifampicin, actinomycin D. ethidium bromide or neutral acriflavin, inhibit the transcription activity to some extent. The transcription products analysed by sucrose gradient centrifugation shows the presence of 3 RNA peaks. This results suggest a possible presence of RNA polymerase associated with Ag-NPV polyhedra.
650 $abiological control
650 $aBrazil
650 $aControle Biológico
650 $aVírus
653 $aAnticarsia germmatalis
653 $aBrasil
653 $aInsects pest
653 $aInsetos pragas
653 $aNuclear polyhedrosis virus
653 $aPoliedrose nuclear
700 1 $aMOUSSATCHE, N.
700 1 $aMOSCARDI, F.
700 1 $aREBELLO, M. A.
773 $tIn: ENCONTRO NACIONAL DE VIROLOGIA, 3., 1986, Sao Lourenco. Resumos... Rio de Janeiro: Centro Panamericano de Febre Aftosa, 1986. p.103.
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Embrapa Soja (CNPSO) |
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