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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
16/03/2011 |
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Data da última atualização: |
22/03/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COSTA, D. C. S.; BELO, V. A.; SILVA, P. S.; METZGER, I. F.; LANNA, C. M.; MACHADO, M. A.; SANTOS, J. E. T. |
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Afiliação: |
D. C. SOUZA COSTA, UFJF; V. A. BELO, UNICAMP; P. S. SILVA, UNICAMP; I. F. METZGER, FMRP/USP; C. M. LANNA, UFJF; MARCO ANTONIO MACHADO, CNPGL; J. E. TANUS SANTOS, FMRP/USP. |
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Título: |
eNOS haplotype associated with hypertension in obese children and adolescents. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
International Journal of Obesity, v. 35, p. 387-392, 2010. |
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DOI: |
https://doi.org/10.1038/ijo.2010.146 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of our study is to investigate whether genetic polymorphisms in the endothelial nitric oxide synthase (eNOS) gene (in the promoter region T−786C, in exon 7 (Glu298Asp) and in intron 4 (4b/4a)) or eNOS haplotypes are associated with hypertension in obese children and adolescents. Methods: We genotyped 175 healthy (controls), 110 normotensive obese and 73 hypertensive obese children and adolescents. Genotypes were determined by Taqman allele discrimination assay and real-time PCR, and by PCR followed by fragment separation by electrophoresis. We compared the distribution of eNOS genotypes, alleles and haplotypes in the three study groups of subjects. We have also measured whole-blood nitrite concentrations. Results: The 4a4a genotype for the intron 4 polymorphism was more common in normotensive obese and hypertensive obese (P<0.01). The AspAsp genotype for Glu298Asp polymorphism was less common in normotensive obese (P<0.02). No significant differences were found in allele distributions for the three eNOS polymorphisms. However, the haplotype combining the C, 4b and Glu variants for the three polymorphisms was more common in hypertensive obese than in normotensive obese or control children and adolescents (odds ratio=2.28 and 2.79, respectively; 95% confidence interval: 1.31–4.31 and 1.39–5.64, respectively; both P<0.00625). This haplotype was not associated with significantly different nitrite concentrations (P>0.05). Conclusions:Our findings suggest that the eNOS haplotype, C b Glu, is associated with hypertension in obese children and adolescents. Further studies examining the possible interactions of eNOS haplotypes with environmental factors and other genetic markers involved in the development of obesity and its complications are warranted. MenosThe aim of our study is to investigate whether genetic polymorphisms in the endothelial nitric oxide synthase (eNOS) gene (in the promoter region T−786C, in exon 7 (Glu298Asp) and in intron 4 (4b/4a)) or eNOS haplotypes are associated with hypertension in obese children and adolescents. Methods: We genotyped 175 healthy (controls), 110 normotensive obese and 73 hypertensive obese children and adolescents. Genotypes were determined by Taqman allele discrimination assay and real-time PCR, and by PCR followed by fragment separation by electrophoresis. We compared the distribution of eNOS genotypes, alleles and haplotypes in the three study groups of subjects. We have also measured whole-blood nitrite concentrations. Results: The 4a4a genotype for the intron 4 polymorphism was more common in normotensive obese and hypertensive obese (P<0.01). The AspAsp genotype for Glu298Asp polymorphism was less common in normotensive obese (P<0.02). No significant differences were found in allele distributions for the three eNOS polymorphisms. However, the haplotype combining the C, 4b and Glu variants for the three polymorphisms was more common in hypertensive obese than in normotensive obese or control children and adolescents (odds ratio=2.28 and 2.79, respectively; 95% confidence interval: 1.31–4.31 and 1.39–5.64, respectively; both P<0.00625). This haplotype was not associated with significantly different nitrite concentrations (P>0.05). Conclusions:Our findings suggest that the eNOS hap... Mostrar Tudo |
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Palavras-Chave: |
ENOS. |
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Thesaurus Nal: |
children; haplotypes; hypertension; nitric oxide. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/881116/1/eNOS-haplotype-associated-with-hypertension.pdf
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Marc: |
LEADER 02551naa a2200265 a 4500
001 1881116
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024 7 $ahttps://doi.org/10.1038/ijo.2010.146$2DOI
100 1 $aCOSTA, D. C. S.
245 $aeNOS haplotype associated with hypertension in obese children and adolescents.$h[electronic resource]
260 $c2010
520 $aThe aim of our study is to investigate whether genetic polymorphisms in the endothelial nitric oxide synthase (eNOS) gene (in the promoter region T−786C, in exon 7 (Glu298Asp) and in intron 4 (4b/4a)) or eNOS haplotypes are associated with hypertension in obese children and adolescents. Methods: We genotyped 175 healthy (controls), 110 normotensive obese and 73 hypertensive obese children and adolescents. Genotypes were determined by Taqman allele discrimination assay and real-time PCR, and by PCR followed by fragment separation by electrophoresis. We compared the distribution of eNOS genotypes, alleles and haplotypes in the three study groups of subjects. We have also measured whole-blood nitrite concentrations. Results: The 4a4a genotype for the intron 4 polymorphism was more common in normotensive obese and hypertensive obese (P<0.01). The AspAsp genotype for Glu298Asp polymorphism was less common in normotensive obese (P<0.02). No significant differences were found in allele distributions for the three eNOS polymorphisms. However, the haplotype combining the C, 4b and Glu variants for the three polymorphisms was more common in hypertensive obese than in normotensive obese or control children and adolescents (odds ratio=2.28 and 2.79, respectively; 95% confidence interval: 1.31–4.31 and 1.39–5.64, respectively; both P<0.00625). This haplotype was not associated with significantly different nitrite concentrations (P>0.05). Conclusions:Our findings suggest that the eNOS haplotype, C b Glu, is associated with hypertension in obese children and adolescents. Further studies examining the possible interactions of eNOS haplotypes with environmental factors and other genetic markers involved in the development of obesity and its complications are warranted.
650 $achildren
650 $ahaplotypes
650 $ahypertension
650 $anitric oxide
653 $aENOS
700 1 $aBELO, V. A.
700 1 $aSILVA, P. S.
700 1 $aMETZGER, I. F.
700 1 $aLANNA, C. M.
700 1 $aMACHADO, M. A.
700 1 $aSANTOS, J. E. T.
773 $tInternational Journal of Obesity$gv. 35, p. 387-392, 2010.
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Embrapa Gado de Leite (CNPGL) |
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| 1. |  | COSTA, D. C. S.; BELO, V. A.; SILVA, P. S.; METZGER, I. F.; LANNA, C. M.; MACHADO, M. A.; SANTOS, J. E. T. eNOS haplotype associated with hypertension in obese children and adolescents. International Journal of Obesity, v. 35, p. 387-392, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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