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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
05/11/2008 |
Data da última atualização: |
20/09/2013 |
Autoria: |
MESSINA, M. A. |
Afiliação: |
MIGUEL ANGEL MESSINA, INTA. |
Título: |
Viaje de intercambio cientifico al Japon, relacionado con "cancrosis" y virus en citrus. |
Ano de publicação: |
1980 |
Fonte/Imprenta: |
República Argentina: INTA, 1980. |
Páginas: |
14p. |
Série: |
(INTA. Informes especiales, 2). |
ISSN: |
0325-9412 |
Idioma: |
Espanhol |
Conteúdo: |
En junio de 1978, a propuesta del Dr. Masao Goto profesor de la Faculdad de Agricultura de la Universidad de Shizuoka y del Dr. Ralph E. Schwarz Jefe del proyecto argentino-alemán para el fomento de la citricultura en la provincia de Misiones-se realizó un viaje de documentación y estudio al Japón con el fin de interiorizarse de los progresos logrados en la investigación sobre la "cancrosis" de los citrus. El mismo se dividió en dos etapas. La primeira - que tuvo una duración de 14 dias - compredió una serie de visitas a todas las Estaciones Experimentales y Universidades relacionadas con la investigación de la "cancrosis" y enfermedades a virus que atacan los citrus. La segunda abarcó los 21 dias restantes y se desarrolló exclusivamente en los laboratorios a cargo del Dr. Goto en Shizuoka, donde se efectuaron trabajos experimentales con la bacteria Xanthomonas citri y sus bacteriófagos y se imtercambiaron ideas sobre los problemas básicas que habria que resolver en relación con esta enfermedad. |
Thesaurus Nal: |
Citrus. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01466nam a2200157 a 4500 001 1636650 005 2013-09-20 008 1980 bl uuuu u0uu1 u #d 022 $a0325-9412 100 1 $aMESSINA, M. A. 245 $aViaje de intercambio cientifico al Japon, relacionado con "cancrosis" y virus en citrus. 260 $aRepública Argentina: INTA$c1980 300 $a14p. 490 $a(INTA. Informes especiales, 2). 520 $aEn junio de 1978, a propuesta del Dr. Masao Goto profesor de la Faculdad de Agricultura de la Universidad de Shizuoka y del Dr. Ralph E. Schwarz Jefe del proyecto argentino-alemán para el fomento de la citricultura en la provincia de Misiones-se realizó un viaje de documentación y estudio al Japón con el fin de interiorizarse de los progresos logrados en la investigación sobre la "cancrosis" de los citrus. El mismo se dividió en dos etapas. La primeira - que tuvo una duración de 14 dias - compredió una serie de visitas a todas las Estaciones Experimentales y Universidades relacionadas con la investigación de la "cancrosis" y enfermedades a virus que atacan los citrus. La segunda abarcó los 21 dias restantes y se desarrolló exclusivamente en los laboratorios a cargo del Dr. Goto en Shizuoka, donde se efectuaron trabajos experimentales con la bacteria Xanthomonas citri y sus bacteriófagos y se imtercambiaron ideas sobre los problemas básicas que habria que resolver en relación con esta enfermedad. 650 $aCitrus
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
Biblioteca(s): |
Embrapa Solos. |
Data corrente: |
19/10/2021 |
Data da última atualização: |
19/10/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
EVANS, F. F.; ROSADO, A. S.; SEBASTIÁN, G. V.; CASELLA, R.; MACHADO, P. L. O. de A.; HOLMSTRÖM, C.; KJELLEBERG, S.; ELSAS, J. D. van; SELDIN, L. |
Afiliação: |
FLAVIA F. EVANS, UFRJ; ALEXANDRE S. ROSADO, UFRJ; GINA V. SEBASTIÁN, CENPES/PETROBRAS; RENATA CASELLA, CENPES/PETROBRAS; PEDRO LUIZ OLIVEIRA DE A MACHADO, CNPS; CAROLA HOLMSTRÖM, UNIVERSITY OF NEW SOUTH WALES; STAFFAN KJELLEBERG, UNIVERSITY OF NEW SOUTH WALES; JAN D. VAN ELSAS, GRONINGEN UNIVERSITY; LUCY SELDIN, UFRJ. |
Título: |
Impact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
FEMS Microbiology Ecology, v. 49, n. 2, p. 295-305, Aug. 2004. |
DOI: |
https://doi.org/10.1016/j.femsec.2004.04.007 |
Idioma: |
Inglês |
Notas: |
Na publicação: Pedro L. O. A. Machado. |
Conteúdo: |
The aim of this study was to analyse the effect of oil contamination and biostimulation (soil pH raise, and nitrogen, phosphate and sulphur addition) on the diversity of a bacterial community of an acidic Cambisol under Atlantic Forest. The experiment was based on the enumeration of bacterial populations and hydrocarbon degraders in microcosms through the use of conventional plating techniques and molecular fingerprinting of samples directly from the environment. PCR followed by denaturing gradient gel electrophoresis (DGGE) was used to generate microbial community fingerprints employing 16S rRNA gene as molecular marker. Biostimulation led to increases of soil pH (to 7.0) and of the levels of phosphorus and K, Ca, and Mg. Oil contamination caused an increase in soil organic carbon (170-190% higher than control soil). Total bacterial counts were stable throughout the experiment, while MPN counts of hydrocarbon degraders showed an increase in the biostimulated and oil-contaminated soil samples. Molecular fingerprinting performed with 16S rRNA gene PCR and DGGE analysis revealed stable patterns along the 360 days of experiment, showing little change in oil-contaminated microcosms after 90 days. The DGGE patterns of the biostimulated samples showed severe changes due to decreases in the number of bands as compared to the control samples as from 15 days after addition of nutrients to the soil. Results obtained in the present study indicate that the addition of inorganic compounds to soil in conjunction with oil contamination has a greater impact on the bacterial community than oil contamination only. MenosThe aim of this study was to analyse the effect of oil contamination and biostimulation (soil pH raise, and nitrogen, phosphate and sulphur addition) on the diversity of a bacterial community of an acidic Cambisol under Atlantic Forest. The experiment was based on the enumeration of bacterial populations and hydrocarbon degraders in microcosms through the use of conventional plating techniques and molecular fingerprinting of samples directly from the environment. PCR followed by denaturing gradient gel electrophoresis (DGGE) was used to generate microbial community fingerprints employing 16S rRNA gene as molecular marker. Biostimulation led to increases of soil pH (to 7.0) and of the levels of phosphorus and K, Ca, and Mg. Oil contamination caused an increase in soil organic carbon (170-190% higher than control soil). Total bacterial counts were stable throughout the experiment, while MPN counts of hydrocarbon degraders showed an increase in the biostimulated and oil-contaminated soil samples. Molecular fingerprinting performed with 16S rRNA gene PCR and DGGE analysis revealed stable patterns along the 360 days of experiment, showing little change in oil-contaminated microcosms after 90 days. The DGGE patterns of the biostimulated samples showed severe changes due to decreases in the number of bands as compared to the control samples as from 15 days after addition of nutrients to the soil. Results obtained in the present study indicate that the addition of inorganic compound... Mostrar Tudo |
Palavras-Chave: |
16S rRNA gene; Atlantic forest; Bacterial diversity; Biostimulation; Crude oil; DGGE; Soil microcosms. |
Thesaurus NAL: |
Cambisols. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227068/1/Impact-of-oil-contamination-and-biostimulation-2004.pdf
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Marc: |
LEADER 02661naa a2200337 a 4500 001 2135450 005 2021-10-19 008 2004 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.femsec.2004.04.007$2DOI 100 1 $aEVANS, F. F. 245 $aImpact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms.$h[electronic resource] 260 $c2004 500 $aNa publicação: Pedro L. O. A. Machado. 520 $aThe aim of this study was to analyse the effect of oil contamination and biostimulation (soil pH raise, and nitrogen, phosphate and sulphur addition) on the diversity of a bacterial community of an acidic Cambisol under Atlantic Forest. The experiment was based on the enumeration of bacterial populations and hydrocarbon degraders in microcosms through the use of conventional plating techniques and molecular fingerprinting of samples directly from the environment. PCR followed by denaturing gradient gel electrophoresis (DGGE) was used to generate microbial community fingerprints employing 16S rRNA gene as molecular marker. Biostimulation led to increases of soil pH (to 7.0) and of the levels of phosphorus and K, Ca, and Mg. Oil contamination caused an increase in soil organic carbon (170-190% higher than control soil). Total bacterial counts were stable throughout the experiment, while MPN counts of hydrocarbon degraders showed an increase in the biostimulated and oil-contaminated soil samples. Molecular fingerprinting performed with 16S rRNA gene PCR and DGGE analysis revealed stable patterns along the 360 days of experiment, showing little change in oil-contaminated microcosms after 90 days. The DGGE patterns of the biostimulated samples showed severe changes due to decreases in the number of bands as compared to the control samples as from 15 days after addition of nutrients to the soil. Results obtained in the present study indicate that the addition of inorganic compounds to soil in conjunction with oil contamination has a greater impact on the bacterial community than oil contamination only. 650 $aCambisols 653 $a16S rRNA gene 653 $aAtlantic forest 653 $aBacterial diversity 653 $aBiostimulation 653 $aCrude oil 653 $aDGGE 653 $aSoil microcosms 700 1 $aROSADO, A. S. 700 1 $aSEBASTIÁN, G. V. 700 1 $aCASELLA, R. 700 1 $aMACHADO, P. L. O. de A. 700 1 $aHOLMSTRÖM, C. 700 1 $aKJELLEBERG, S. 700 1 $aELSAS, J. D. van 700 1 $aSELDIN, L. 773 $tFEMS Microbiology Ecology$gv. 49, n. 2, p. 295-305, Aug. 2004.
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