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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
16/08/1993 |
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Data da última atualização: |
15/02/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
VALARINI, P. J.; MENTEN, J. O. M. |
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Afiliação: |
PEDRO JOSE VALARINI, EMBRAPA-CNPDA; USP/PIRACICABA. |
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Título: |
Routine detection of bacterial blight in common bean seeds by inoculation of an indicator plant. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
In: INTERNATIONAL PLANT PROTECTION CONGRESS, 12., 1991, Rio de Janeiro. Oral and poster sessions - program and abstracts. Rio de Janeiro: [s.n.], 1991. |
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Páginas: |
n.p |
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Idioma: |
Inglês |
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Conteúdo: |
Seedborne inoculum of Xanthomonas campestris pv. phaseoli is an important mean of the pathogen dissemination and for epidemic disease development. The comparison of four bacterial extraction techniques and three identification methods showed that the pathogen extraction by soaking whole seeds in sterile destilled water for 18-24 hrs, at 5.C and inoculation of an indicator plant was the most appropriate procedure for detection of pathogen in bean seeds. Indicator plant test was evaluatet for sensibility of 0,1%, reproductibility, high specificity, low cost and easy execution. It is possible to determine an estimated percentage of infected/contaminated seeds in a sample; a technician can analyse 10 sample/day, obtaining results in 8-10 days. A survey of certified bean seeds produced in the state of Sao Paulo, Brazil, from 1988, dry and winter seasons and 1988/89, rainy season showed most of the samples carrying the pathogen (levels varying from 0,1 to 1,1%). Its application is recommended in routine seed health testing and in programmes of seed certification in tropical developing countries. |
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Palavras-Chave: |
Disease; Inoculation; phaseoli; Xanthomonas campestris pv. |
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Thesagro: |
Bactéria. |
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Thesaurus Nal: |
beans; detection; seeds. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/10812/1/1991PC-Valarini-Routine-1455.PDF
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Marc: |
LEADER 01848nam a2200229 a 4500
001 1010812
005 2023-02-15
008 1991 bl uuuu u00u1 u #d
100 1 $aVALARINI, P. J.
245 $aRoutine detection of bacterial blight in common bean seeds by inoculation of an indicator plant.$h[electronic resource]
260 $aIn: INTERNATIONAL PLANT PROTECTION CONGRESS, 12., 1991, Rio de Janeiro. Oral and poster sessions - program and abstracts. Rio de Janeiro: [s.n.]$c1991
300 $an.p
520 $aSeedborne inoculum of Xanthomonas campestris pv. phaseoli is an important mean of the pathogen dissemination and for epidemic disease development. The comparison of four bacterial extraction techniques and three identification methods showed that the pathogen extraction by soaking whole seeds in sterile destilled water for 18-24 hrs, at 5.C and inoculation of an indicator plant was the most appropriate procedure for detection of pathogen in bean seeds. Indicator plant test was evaluatet for sensibility of 0,1%, reproductibility, high specificity, low cost and easy execution. It is possible to determine an estimated percentage of infected/contaminated seeds in a sample; a technician can analyse 10 sample/day, obtaining results in 8-10 days. A survey of certified bean seeds produced in the state of Sao Paulo, Brazil, from 1988, dry and winter seasons and 1988/89, rainy season showed most of the samples carrying the pathogen (levels varying from 0,1 to 1,1%). Its application is recommended in routine seed health testing and in programmes of seed certification in tropical developing countries.
650 $abeans
650 $adetection
650 $aseeds
650 $aBactéria
653 $aDisease
653 $aInoculation
653 $aphaseoli
653 $aXanthomonas campestris pv
700 1 $aMENTEN, J. O. M.
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Embrapa Meio Ambiente (CNPMA) |
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