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Registro Completo |
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
27/01/2009 |
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Data da última atualização: |
27/01/2009 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CAZÉ, A. L. R.; LUCENA, V. S.; MENEZES, I. P. P. de; BARROSO, P. A. V.; RIBEIRO, J. L. |
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Afiliação: |
Ana Luíza Ramos Cazé, UEPB; Valeska Silva Lucena, estagiária; Ivandilson Pessoa Pinto de Menezes, UFRN; Paulo Augusto Vianna Barroso, Embrapa Algodão. |
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Título: |
Avaliação qualitativa in situ dos acessos de algodoeiros coletados no Estado do Maranhão. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE BOTÂNICA, 59.; REUNIÃO NORDESTINA DE BOTÂNICA, 31.; CONGRESSO LATINOAMERICANO Y DEL CARIBE DE CACTÁCEAS Y OTRAS SUCULENTAS, 4.; CONGRESS OF INTERNATIONAL ORGANIZATION FOR SUCULENT PLANT STUDY, 30., 2008, Natal. Atualidades, desafios e perspectivas da botânica no Brasil: resumos. Natal: UFERSA: UFRN: SBB, 2008. |
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Idioma: |
Português |
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Categoria do assunto: |
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Marc: |
LEADER 00775naa a2200157 a 4500
001 1277260
005 2009-01-27
008 2008 bl --- 0-- u #d
100 1 $aCAZÉ, A. L. R.
245 $aAvaliação qualitativa in situ dos acessos de algodoeiros coletados no Estado do Maranhão.
260 $c2008
700 1 $aLUCENA, V. S.
700 1 $aMENEZES, I. P. P. de
700 1 $aBARROSO, P. A. V.
700 1 $aRIBEIRO, J. L.
773 $tIn: CONGRESSO NACIONAL DE BOTÂNICA, 59.; REUNIÃO NORDESTINA DE BOTÂNICA, 31.; CONGRESSO LATINOAMERICANO Y DEL CARIBE DE CACTÁCEAS Y OTRAS SUCULENTAS, 4.; CONGRESS OF INTERNATIONAL ORGANIZATION FOR SUCULENT PLANT STUDY, 30., 2008, Natal. Atualidades, desafios e perspectivas da botânica no Brasil: resumos. Natal: UFERSA: UFRN: SBB, 2008.
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Registro original: |
Embrapa Algodão (CNPA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
15/06/2016 |
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Data da última atualização: |
15/06/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
MORAES, D. M. C.; DUARTE, S. C.; BASTOS, T. S. A.; REZENDE, C. L. G.; LEANDRO, N. S. M.; CAFÉ, M. B.; STRINGHINI, J. H.; ANDRADE, M. A. |
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Afiliação: |
DUNYA MARA CARDOSO MORAES, UFG/Escola de Veterinária e Zootecnia; SABRINA CASTILHO DUARTE, CNPSA; UFG/Escola de Veterinária e Zootecnia; UFG/Escola de Veterinária e Zootecnia; UFG/Escola de Veterinária e Zootecnia; UFG/Escola de Veterinária e Zootecnia; JOSÉ HENRIQUE STRINGHINI, UFG/Escola de Veterinária e Zootecnia; MARIA AUXILIADORA ANDRADE, UFG/Escola de Veterinária e Zootecnia. |
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Título: |
Detection of Salmonella spp. by Conventional Bacteriology and by Quantitative Polymerase-Chain Reaction in Commercial Egg Structures. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Brazilian Journal of Poultry Science, v. 18, n.1, p. 117-124, 2016. |
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DOI: |
http://dx.doi.org/10.1590/18069061-2015-006 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Conventional bacteriology techniques and quantitative polymerasechain reaction (qPCR) were applied to the eggshell, albumen, and yolk of washed and unwashed commercial white and brown eggs, to detect Salmonella spp. Pooled samples of eggshells, albumen, and yolk of white and brown eggs were collected at the poultry house and at the egg-storage room. Salmonella spp. was detected by conventional bacteriology in 5.4% (21/387) of analyzed samples and in 16% (68/387) by qPCR. In the 114 unwashed white eggs samples of eggshell, albumen and yolk, the bacterium was identified in 2.6% of the eggs (3/114) by conventional bacteriology and in 13.2% (15/114) by qPCR. In the 90 samples of washed eggs, 6.7% (6/90) were contaminated as detected by conventional bacteriology and 10.0% (9/90) by qPCR. In the 81 samples of unwashed brown eggs, Salmonella spp. was detected in 6.1% of the eggs (5/81) by conventional bacteriology and 27.2% (22/81) by qPCR. In the 102 samples of brown washed eggs, 6.9% (7/102) where positive by conventional bacteriology and 35.3% (16/102) by qPCR. All samples detected as positive by conventional bacteriology were also positive by qPCR. Salmonella Agona represented 18.2% (4/22) of identified serovars, Salmonella enterica subs. enterica O: 4.5 18.2% (4/22), Salmonella Schwarzengrund 18.2% (4/22), Salmonella Cerro 13.6% (3/22), Salmonella Anatum 13.6% (3/22), Salmonella Enteritidis 9.1% (2/22), Salmonella Johannesburg 4.5% (1/22), and Salmonella Corvallis 4.5% (1/22). The qPCR method provided better detection of Salmonella spp. in commercial eggs than conventional bacteriology. The conventional egg washing and disinfection procedures are not efficient to eliminate Salmonella. MenosAbstract: Conventional bacteriology techniques and quantitative polymerasechain reaction (qPCR) were applied to the eggshell, albumen, and yolk of washed and unwashed commercial white and brown eggs, to detect Salmonella spp. Pooled samples of eggshells, albumen, and yolk of white and brown eggs were collected at the poultry house and at the egg-storage room. Salmonella spp. was detected by conventional bacteriology in 5.4% (21/387) of analyzed samples and in 16% (68/387) by qPCR. In the 114 unwashed white eggs samples of eggshell, albumen and yolk, the bacterium was identified in 2.6% of the eggs (3/114) by conventional bacteriology and in 13.2% (15/114) by qPCR. In the 90 samples of washed eggs, 6.7% (6/90) were contaminated as detected by conventional bacteriology and 10.0% (9/90) by qPCR. In the 81 samples of unwashed brown eggs, Salmonella spp. was detected in 6.1% of the eggs (5/81) by conventional bacteriology and 27.2% (22/81) by qPCR. In the 102 samples of brown washed eggs, 6.9% (7/102) where positive by conventional bacteriology and 35.3% (16/102) by qPCR. All samples detected as positive by conventional bacteriology were also positive by qPCR. Salmonella Agona represented 18.2% (4/22) of identified serovars, Salmonella enterica subs. enterica O: 4.5 18.2% (4/22), Salmonella Schwarzengrund 18.2% (4/22), Salmonella Cerro 13.6% (3/22), Salmonella Anatum 13.6% (3/22), Salmonella Enteritidis 9.1% (2/22), Salmonella Johannesburg 4.5% (1/22), and Salmonella Corvallis 4.... Mostrar Tudo |
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Palavras-Chave: |
QPCR. |
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Thesagro: |
Bacteriologia; Casca de ovo; Gema de ovo; Salmonella. |
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Thesaurus NAL: |
Egg albumen; Egg shell; Egg yolk; Quantitative polymerase chain reaction. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02730naa a2200325 a 4500
001 2047116
005 2016-06-15
008 2016 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1590/18069061-2015-006$2DOI
100 1 $aMORAES, D. M. C.
245 $aDetection of Salmonella spp. by Conventional Bacteriology and by Quantitative Polymerase-Chain Reaction in Commercial Egg Structures.$h[electronic resource]
260 $c2016
520 $aAbstract: Conventional bacteriology techniques and quantitative polymerasechain reaction (qPCR) were applied to the eggshell, albumen, and yolk of washed and unwashed commercial white and brown eggs, to detect Salmonella spp. Pooled samples of eggshells, albumen, and yolk of white and brown eggs were collected at the poultry house and at the egg-storage room. Salmonella spp. was detected by conventional bacteriology in 5.4% (21/387) of analyzed samples and in 16% (68/387) by qPCR. In the 114 unwashed white eggs samples of eggshell, albumen and yolk, the bacterium was identified in 2.6% of the eggs (3/114) by conventional bacteriology and in 13.2% (15/114) by qPCR. In the 90 samples of washed eggs, 6.7% (6/90) were contaminated as detected by conventional bacteriology and 10.0% (9/90) by qPCR. In the 81 samples of unwashed brown eggs, Salmonella spp. was detected in 6.1% of the eggs (5/81) by conventional bacteriology and 27.2% (22/81) by qPCR. In the 102 samples of brown washed eggs, 6.9% (7/102) where positive by conventional bacteriology and 35.3% (16/102) by qPCR. All samples detected as positive by conventional bacteriology were also positive by qPCR. Salmonella Agona represented 18.2% (4/22) of identified serovars, Salmonella enterica subs. enterica O: 4.5 18.2% (4/22), Salmonella Schwarzengrund 18.2% (4/22), Salmonella Cerro 13.6% (3/22), Salmonella Anatum 13.6% (3/22), Salmonella Enteritidis 9.1% (2/22), Salmonella Johannesburg 4.5% (1/22), and Salmonella Corvallis 4.5% (1/22). The qPCR method provided better detection of Salmonella spp. in commercial eggs than conventional bacteriology. The conventional egg washing and disinfection procedures are not efficient to eliminate Salmonella.
650 $aEgg albumen
650 $aEgg shell
650 $aEgg yolk
650 $aQuantitative polymerase chain reaction
650 $aBacteriologia
650 $aCasca de ovo
650 $aGema de ovo
650 $aSalmonella
653 $aQPCR
700 1 $aDUARTE, S. C.
700 1 $aBASTOS, T. S. A.
700 1 $aREZENDE, C. L. G.
700 1 $aLEANDRO, N. S. M.
700 1 $aCAFÉ, M. B.
700 1 $aSTRINGHINI, J. H.
700 1 $aANDRADE, M. A.
773 $tBrazilian Journal of Poultry Science$gv. 18, n.1, p. 117-124, 2016.
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Embrapa Suínos e Aves (CNPSA) |
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