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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
20/09/2001 |
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Data da última atualização: |
20/09/2001 |
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Autoria: |
HERNANDEZ, L.; SOTOLONGO, M.; ROSABAL, Y.; MENENDEZ, C.; RAMIREZ, R.; CABALLERO-MELLADO, J.; ARRIETA, J. |
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Título: |
Structural levansucrase gene (lsd A) constitutes a functional locus conserved in the species gluconacetobacter diazotrophicus. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Archives of Microbiology, London, v. 174, p. 120-124, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
Levansucrase (EC 2.4.1.10) was identified as a constitutive exoenzyme in 14 Gluconacetobacter diazotrophicus strains recovered from different host plants in diverse geographical regions. The enzyme, consisting of a single 60-kDa polypeptide, hydrolysed sucrose to synthesise oligofructans and levan. Sugar-cane-associated strains of the most abundant genotype (electrophoretic type 1) showed maximal values of levansucrase production. These values were three-fold higher than those of the isolates recovered from coffe plants. Restriction fragment length polymorphism analysis revealed a high degree of conservation of the levansucrase locus (lsdA) among the 14 strains under study, which representes 11 different G. diazotrophicus genotypes. Targeted disruption of the lsdA. gene in four representative strains abolished their ability to grow on sucrose, indicating that the endophytic species G.diazotrophicus utilises plant sucrose via levansucrase. |
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Palavras-Chave: |
Acetobacter diazotrophicus; Cana-de-açúcar; Endofito. |
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Thesagro: |
Bactéria; Eletroforese. |
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Thesaurus Nal: |
electrophoresis; endophytes; Gluconacetobacter diazotrophicus; sugarcane. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01833naa a2200301 a 4500
001 1621277
005 2001-09-20
008 2000 bl --- 0-- u #d
100 1 $aHERNANDEZ, L.
245 $aStructural levansucrase gene (lsd A) constitutes a functional locus conserved in the species gluconacetobacter diazotrophicus.
260 $c2000
520 $aLevansucrase (EC 2.4.1.10) was identified as a constitutive exoenzyme in 14 Gluconacetobacter diazotrophicus strains recovered from different host plants in diverse geographical regions. The enzyme, consisting of a single 60-kDa polypeptide, hydrolysed sucrose to synthesise oligofructans and levan. Sugar-cane-associated strains of the most abundant genotype (electrophoretic type 1) showed maximal values of levansucrase production. These values were three-fold higher than those of the isolates recovered from coffe plants. Restriction fragment length polymorphism analysis revealed a high degree of conservation of the levansucrase locus (lsdA) among the 14 strains under study, which representes 11 different G. diazotrophicus genotypes. Targeted disruption of the lsdA. gene in four representative strains abolished their ability to grow on sucrose, indicating that the endophytic species G.diazotrophicus utilises plant sucrose via levansucrase.
650 $aelectrophoresis
650 $aendophytes
650 $aGluconacetobacter diazotrophicus
650 $asugarcane
650 $aBactéria
650 $aEletroforese
653 $aAcetobacter diazotrophicus
653 $aCana-de-açúcar
653 $aEndofito
700 1 $aSOTOLONGO, M.
700 1 $aROSABAL, Y.
700 1 $aMENENDEZ, C.
700 1 $aRAMIREZ, R.
700 1 $aCABALLERO-MELLADO, J.
700 1 $aARRIETA, J.
773 $tArchives of Microbiology, London$gv. 174, p. 120-124, 2000.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| 1. |  | PEREIRA, P. A. A.; MENENDEZ, C.; GEPTS, P.; BUTTERY, B.; PARK, S. Mapeamento do gene não-nodulante do feijoeiro na população midnight x R99. In: REUNIÃO NACIONAL DE PESQUISA DE FEIJÃO, 5., 1996, Goiânia. Anais. Goiânia: EMBRAPA-CNPAF, 1996. v. 1. p. 396-397. (EMBRAPA-CNPAF. Documentos, 69).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 1 | |
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