Data/hora: 12/06/2024 05:26:38

Biblioteca(s): | Embrapa Uva e Vinho.
Data corrente: | 22/05/2012
Data da última atualização: | 22/05/2012
Autoria: | SILVA, G. A. da; BERNARDI, T. L.; SCHAKER, P. D. C.; MENEGOTTO, M.; VALENTE, P.
Afiliação: | GILDO ALMEIDA DA SILVA, CNPUV; Taís Letícia Bernardi, CNPUV (BOLSISTA); Patrícia Dayane Carvalho Schaker, CNPUV (BOLSISTA); Morgana Menegotto, CNPUV (BOLSISTA); Patricia Valente, UFRGS.
Título: | Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification.
Ano de publicação: | 2012
Fonte/Imprenta: | Brazilian Archives of Biology and Technology, Curitiba, v. 55, n. 2, p. 319-327, mar./abr. 2012.
Idioma: | Inglês
Conteúdo: | The purpose of this work was to study a rapid yeast DNA extraction by boiling and freeze-thawing processes without using chemical reagents or any purification procedures, to obtain a high grade PCR-product. A specific DNA fragment of the 18S region of Dekkera bruxellensis and Saccharomyces cerevisiae was chosen. The described boiling and freeze-thawing protocols generated the PCR-grade product preparations and could be used to process many samples. The amplification of the fragments could be observed after 30 and 35 cycles. These processes of extraction without using any kind of chemical reagents, especial water, and purification procedures proved to be efficient, reproducible, simple, fast, and inexpensive.
Palavras-Chave: | Ebulição; Extração de DNA; Gelo-degelo; Metodologia.
Thesagro: | DNA; Microbiologia; Saccharomyces Cerevisiae.
Thesaurus Nal: | Dekkera bruxellensis.
Categoria do assunto: | --

URL:

http://www.scielo.br/pdf/babt/v55n2/20.pdf
http://ainfo.cnptia.embrapa.br/digital/bitstream/item/59826/1/SILVA-BrazArchBiolTech-v55n2p319-2012.pdf

Registro original: | Embrapa Uva e Vinho (CNPUV)
Exemplares: | CNPUV - (13901-1) - UPC - AP - DD - Online -  -  - DIS