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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
04/12/2015 |
Data da última atualização: |
24/03/2016 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
SANTOS, K. C. F. dos; HORA, R. A. C. da; CARVALHO, M. de J. da S. de; SOUZA, A. da S.; SILVEIRA, D. M. de S.; MENDONÇA, J. de O. |
Afiliação: |
KAREN CRISTINA FIALHO DOS SANTOS, CNPMF; RAQUEL ALMEIDA CARDOSO DA HORA; MARIANE DE JESUS DA SILVA DE CARVALHO; ANTONIO DA SILVA SOUZA, CNPMF; DEYSE MARIA DE SOUZA SILVEIRA; JACKSON DE OLIVEIRA MENDONÇA. |
Título: |
Micropropagação de acessos de mandioca mediante microestacas coletadas de diferentes posições na planta in vitro. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
CONGRESSO BRASILEIRO DE MANDIOCA E FRUTICULTURA, 16., CONGRESSO LATINO-AMERICANO E CARIBENHO DE MANDIOCA, 2015, Foz do Iguaçu. Integração: segurança alimentar e geração de renda: anais. Foz do Iguaçu: SBM, 2015. 1 CD-ROM. |
Idioma: |
Português |
Conteúdo: |
O trabalho teve por objetivo avaliar o desenvolvimento in vitro de genótipos de mandioca utilizando microestacas coletadas em diferentes posições em plantas previamente cultivadas in vitro. Para isso, microestacas (apicais, basais, enraizadas, medianas e pequenas), com um tamanho aproximado de 1 cm, provenientes de plantas de cinco genótipos de mandioca (BGM 0133, BGM 0947, BGM 1325, BGM 1345 e ?Milagrosa?), foram inoculadas em tubos de ensaio contendo o meio de cultura MS001. |
Palavras-Chave: |
Multiplicação in vitro; Tipos de explante. |
Thesagro: |
Manihot Esculenta. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01362nam a2200205 a 4500 001 2030750 005 2016-03-24 008 2015 bl uuuu u00u1 u #d 100 1 $aSANTOS, K. C. F. dos 245 $aMicropropagação de acessos de mandioca mediante microestacas coletadas de diferentes posições na planta in vitro.$h[electronic resource] 260 $aCONGRESSO BRASILEIRO DE MANDIOCA E FRUTICULTURA, 16., CONGRESSO LATINO-AMERICANO E CARIBENHO DE MANDIOCA, 2015, Foz do Iguaçu. Integração: segurança alimentar e geração de renda: anais. Foz do Iguaçu: SBM, 2015. 1 CD-ROM.$c2015 520 $aO trabalho teve por objetivo avaliar o desenvolvimento in vitro de genótipos de mandioca utilizando microestacas coletadas em diferentes posições em plantas previamente cultivadas in vitro. Para isso, microestacas (apicais, basais, enraizadas, medianas e pequenas), com um tamanho aproximado de 1 cm, provenientes de plantas de cinco genótipos de mandioca (BGM 0133, BGM 0947, BGM 1325, BGM 1345 e ?Milagrosa?), foram inoculadas em tubos de ensaio contendo o meio de cultura MS001. 650 $aManihot Esculenta 653 $aMultiplicação in vitro 653 $aTipos de explante 700 1 $aHORA, R. A. C. da 700 1 $aCARVALHO, M. de J. da S. de 700 1 $aSOUZA, A. da S. 700 1 $aSILVEIRA, D. M. de S. 700 1 $aMENDONÇA, J. de O.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
24/08/2017 |
Data da última atualização: |
23/09/2019 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. |
Afiliação: |
Helena Fabiana Reis de Almeida Saraiva, Universidade de São Paulo (USA) - Pirassununga, SP, Brasil; Ribrio Ivan Tavares Pereira Batista, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brasil; Vivian Angélico Pereira Alfradique, UFF - Niterói, RJ, Brasil; Pedro Henrique Nicolau Pinto, UFF - Niterói, RJ, Brasil; Lilian Santos Ribeiro, UFF - Niterói, RJ, Brasil; CLARA SLADE OLIVEIRA, CNPGL; Joanna Maria Gonçalves de Souza Fabjan, UFF - Niterói, RJ, Brasil; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Zandonadi Brandão, UFF - Niterói, RJ, Brasil. |
Título: |
L-carnitine supplementation during vitrification did not improve survival and quality rates, but altered CrAT and PRDX1 expression in in vivo-produced ovine embryos. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Animal Reprodroduction, v. 14, n. 3, p. 898, Jul./Sept. 2017. |
Idioma: |
Inglês |
Notas: |
Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts. |
Conteúdo: |
Embryo cryodamage is observed mainly at metabolic and molecular aspects and it impairs post warming quality and survival rates. This study aimed to evaluate the effect of L-carnitine (LC) supplementation during either vitrification or post warming solutions on the 6-7th day of in vivo-produced ovine embryos. LC (3.72 mM) was added to vitrification (Experiment 1; C1: control; LC1: supplemented embryos) or warming solutions (Experiment 2; C2; LC2). In vitro culture (IVC) of warmed embryos was performed for 72 h at 38,5 °C, 5% CO2 and 5% O2 to evaluate survival rates in both Experiments. In Experiment 1, reactive oxygen species (ROS) levels were measured by CellROX Green staining, total cell number (TCN) by Hoechst 33342, number of apoptotic cells by caspase-3 immunofluorescence staining protocol, apoptotic index evaluation in both groups. Gene expression analysis of carnitine palmitoyltransferase 1 and 2 (CPT1 and CPT2), carnitine O-acyltransferase (CrAT) and peroxiredoxin 1 (PRDX1), were performed by RT-qPCR (ACTB as endogenous control) in Experiments 1 and 2 and results were compared to fresh embryos (FE). Averages of survival rates were compared by the Chi-Square test. Means of TCN, apoptotic cells, apoptotic index and fluorescence intensity were compared by Student's t-test, at 5% significance level. Survival rates were similar between groups (p> 0.05) in Experiments 1 (68.7%, C1 vs 81.8%, LC1) and 2 (48.5%, C2 vs 64.7%, LC2). In Experiment 1, ROS levels at 24 h of IVC (85.83 ± 68.37 x 1010, C1 vs 89.04 ± 84.48 x 1010, LC1), total cell number at 24 h (89 ± 22, C1 vs 82.2 ± 28, LC1) and 72 h (86 ± 19.9, C1 vs 68.5 ± 25.26, LC1), apoptotic cells (3.75 ± 1.48, C1 vs 4.50 ± 4.72, LC1) and apoptotic index (4.37 ± 1.45, C1 vs 5.23 ± 4.72, LC1) at 72 h of IVC did not differ (p> 0.05) between C1 and LC1. Gene expression analysis showed no differences in CPT1 and CPT2 mRNA relative abundance in embryos of both experiments compared to FE, however, CrAT was downregulated (p< 0.05) in C1 and PRDX1 was downregulated (p< 0.05) in both C1 and LC1, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p< 0.05) in C2 and CrAT was downregulated (p< 0.05) in LC2, in relation to FE. In conclusion, although the short-term LC supplementation at 3.72 mM during cryopreservation did not improve post-warming survival and morphological parameters of the evaluated embryos, it was able to modulate expression of genes related to energy homeostasis (CrAT) and oxidative stress (PRDX1), proving to be beneficial, in both forms of supplementation, to in vivo-produced ovine embryos.". MenosEmbryo cryodamage is observed mainly at metabolic and molecular aspects and it impairs post warming quality and survival rates. This study aimed to evaluate the effect of L-carnitine (LC) supplementation during either vitrification or post warming solutions on the 6-7th day of in vivo-produced ovine embryos. LC (3.72 mM) was added to vitrification (Experiment 1; C1: control; LC1: supplemented embryos) or warming solutions (Experiment 2; C2; LC2). In vitro culture (IVC) of warmed embryos was performed for 72 h at 38,5 °C, 5% CO2 and 5% O2 to evaluate survival rates in both Experiments. In Experiment 1, reactive oxygen species (ROS) levels were measured by CellROX Green staining, total cell number (TCN) by Hoechst 33342, number of apoptotic cells by caspase-3 immunofluorescence staining protocol, apoptotic index evaluation in both groups. Gene expression analysis of carnitine palmitoyltransferase 1 and 2 (CPT1 and CPT2), carnitine O-acyltransferase (CrAT) and peroxiredoxin 1 (PRDX1), were performed by RT-qPCR (ACTB as endogenous control) in Experiments 1 and 2 and results were compared to fresh embryos (FE). Averages of survival rates were compared by the Chi-Square test. Means of TCN, apoptotic cells, apoptotic index and fluorescence intensity were compared by Student's t-test, at 5% significance level. Survival rates were similar between groups (p> 0.05) in Experiments 1 (68.7%, C1 vs 81.8%, LC1) and 2 (48.5%, C2 vs 64.7%, LC2). In Experiment 1, ROS levels at 24 h of IVC (85... Mostrar Tudo |
Palavras-Chave: |
Animal embryos; Santa Inês; Survival. |
Thesaurus NAL: |
Carnitine; Cell culture; Cryopreservation; Ewes; Gene expression; In vitro culture; Sheep; Vitrification. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163011/1/CNPC-2017-L-carnitine.pdf
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Marc: |
LEADER 03898nam a2200361 a 4500 001 2074433 005 2019-09-23 008 2017 bl uuuu u00u1 u #d 100 1 $aSARAIVA, H. F. R. de A. 245 $aL-carnitine supplementation during vitrification did not improve survival and quality rates, but altered CrAT and PRDX1 expression in in vivo-produced ovine embryos.$h[electronic resource] 260 $aAnimal Reprodroduction, v. 14, n. 3, p. 898, Jul./Sept. 2017.$c2017 500 $aProceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts. 520 $aEmbryo cryodamage is observed mainly at metabolic and molecular aspects and it impairs post warming quality and survival rates. This study aimed to evaluate the effect of L-carnitine (LC) supplementation during either vitrification or post warming solutions on the 6-7th day of in vivo-produced ovine embryos. LC (3.72 mM) was added to vitrification (Experiment 1; C1: control; LC1: supplemented embryos) or warming solutions (Experiment 2; C2; LC2). In vitro culture (IVC) of warmed embryos was performed for 72 h at 38,5 °C, 5% CO2 and 5% O2 to evaluate survival rates in both Experiments. In Experiment 1, reactive oxygen species (ROS) levels were measured by CellROX Green staining, total cell number (TCN) by Hoechst 33342, number of apoptotic cells by caspase-3 immunofluorescence staining protocol, apoptotic index evaluation in both groups. Gene expression analysis of carnitine palmitoyltransferase 1 and 2 (CPT1 and CPT2), carnitine O-acyltransferase (CrAT) and peroxiredoxin 1 (PRDX1), were performed by RT-qPCR (ACTB as endogenous control) in Experiments 1 and 2 and results were compared to fresh embryos (FE). Averages of survival rates were compared by the Chi-Square test. Means of TCN, apoptotic cells, apoptotic index and fluorescence intensity were compared by Student's t-test, at 5% significance level. Survival rates were similar between groups (p> 0.05) in Experiments 1 (68.7%, C1 vs 81.8%, LC1) and 2 (48.5%, C2 vs 64.7%, LC2). In Experiment 1, ROS levels at 24 h of IVC (85.83 ± 68.37 x 1010, C1 vs 89.04 ± 84.48 x 1010, LC1), total cell number at 24 h (89 ± 22, C1 vs 82.2 ± 28, LC1) and 72 h (86 ± 19.9, C1 vs 68.5 ± 25.26, LC1), apoptotic cells (3.75 ± 1.48, C1 vs 4.50 ± 4.72, LC1) and apoptotic index (4.37 ± 1.45, C1 vs 5.23 ± 4.72, LC1) at 72 h of IVC did not differ (p> 0.05) between C1 and LC1. Gene expression analysis showed no differences in CPT1 and CPT2 mRNA relative abundance in embryos of both experiments compared to FE, however, CrAT was downregulated (p< 0.05) in C1 and PRDX1 was downregulated (p< 0.05) in both C1 and LC1, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p< 0.05) in C2 and CrAT was downregulated (p< 0.05) in LC2, in relation to FE. In conclusion, although the short-term LC supplementation at 3.72 mM during cryopreservation did not improve post-warming survival and morphological parameters of the evaluated embryos, it was able to modulate expression of genes related to energy homeostasis (CrAT) and oxidative stress (PRDX1), proving to be beneficial, in both forms of supplementation, to in vivo-produced ovine embryos.". 650 $aCarnitine 650 $aCell culture 650 $aCryopreservation 650 $aEwes 650 $aGene expression 650 $aIn vitro culture 650 $aSheep 650 $aVitrification 653 $aAnimal embryos 653 $aSanta Inês 653 $aSurvival 700 1 $aBATISTA, R. I. T. P. 700 1 $aALFRADIQUE, V. A. P. 700 1 $aPINTO, P. H. N. 700 1 $aRIBEIRO, L. S. 700 1 $aOLIVEIRA, C. S. 700 1 $aSOUZA FABJAN, J. M. G. de 700 1 $aCAMARGO, L. S. de A. 700 1 $aFONSECA, J. F. da 700 1 $aBRANDÃO, F. Z.
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