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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
23/03/2020 |
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Data da última atualização: |
27/04/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, C. de O. G.; TEIXEIRA, S. T.; RODRIGUES, K. B.; SOUZA, A. A.; MONCLARO, A. V.; MENDES, T. D.; RIBEIRO, J. A. de A.; SIQUEIRA, F. G. de; FAVARO, L. C. de L.; ABDELNUR, P. V. |
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Afiliação: |
Caio de Oliveira Gorgulho Silva; Tallyta Santos Teixeira, Universidade Federal de Tocantins; Kelly Barreto Rodrigues; Amanda Araujo Souza; Antonielle Vieira Monclaro; THAIS DEMARCHI MENDES, CNPAE; JOSE ANTONIO DE AQUINO RIBEIRO, CNPAE; FELIX GONCALVES DE SIQUEIRA, CNPAE; LEIA CECILIA DE LIMA FAVARO, CNPAE; PATRICIA VERARDI ABDELNUR, CNPAE. |
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Título: |
Combination of MALDI-TOF MS and UHPLC-ESI-MS for the characterization of lytic polysaccharide monooxygenase activity. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Analytical Methods, n. 2, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
Lytic polysaccharide monooxygenases (LPMOs) are redox enzymes of high biotechnological interest due to their capacity to degrade recalcitrant polysaccharides, such as cellulose, by an oxidative mechanism. The characterization of LPMOs is challenging since they generate a variety of catalytic products which include native oligosaccharides (non-oxidized) and oligosaccharides oxidized at the reducing end (C1 position), the non-reducing end (C4 position), or both ends, with different degrees of polymerization. Moreover, oxidized products exist in equilibrium with their hydrated forms, which further complicates their identification. The lack of commercial analytical standards for all these possible forms of oxidized oligosaccharides and the low concentration of these products make LPMO functional characterization dependent on advanced mass spectrometry techniques capable of identifying the profile of oxidized products. Here, a new approach for the characterization of cellulose-active LPMOs based on the combination of MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS was proposed and optimized. The LPMO TrAA9A from Trichoderma reesei was used as a model enzyme to develop and test the MS methods. MALDI-TOF MS and UHPLC-ESI-MS methods were both capable of identifying putative C1, C4 and C1/C4 oxidized cello-oligosaccharides as well as their native counterparts generated after cellulose treatment with LPMO, which allows their utilization to characterize type I (C1-oxidizer), type II (C4-oxidizer) and type III (C1- and C4-oxidizer) LPMOs. Moreover, both methods were complementary since MALDI-TOF MS was capable of detecting oligosaccharides with higher degrees of polymerization (DP3?DP10), while UHPLC-ESI-MS allowed the evaluation of smaller oligosaccharides (DP1?DP5). The combined use of both methods offers a comprehensive description of LPMO catalytic products. MenosLytic polysaccharide monooxygenases (LPMOs) are redox enzymes of high biotechnological interest due to their capacity to degrade recalcitrant polysaccharides, such as cellulose, by an oxidative mechanism. The characterization of LPMOs is challenging since they generate a variety of catalytic products which include native oligosaccharides (non-oxidized) and oligosaccharides oxidized at the reducing end (C1 position), the non-reducing end (C4 position), or both ends, with different degrees of polymerization. Moreover, oxidized products exist in equilibrium with their hydrated forms, which further complicates their identification. The lack of commercial analytical standards for all these possible forms of oxidized oligosaccharides and the low concentration of these products make LPMO functional characterization dependent on advanced mass spectrometry techniques capable of identifying the profile of oxidized products. Here, a new approach for the characterization of cellulose-active LPMOs based on the combination of MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS was proposed and optimized. The LPMO TrAA9A from Trichoderma reesei was used as a model enzyme to develop and test the MS methods. MALDI-TOF MS and UHPLC-ESI-MS methods were both capable of identifying putative C1, C4 and C1/C4 oxidized cello-oligosaccharides as well as their native counterparts generated after cellulose treatment with LPMO, which allows their utilization to characterize type I (C1-oxidizer), type... Mostrar Tudo |
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Palavras-Chave: |
Caracterização de LPMOs. |
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Categoria do assunto: |
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Marc: |
LEADER 02626naa a2200241 a 4500
001 2121326
005 2020-04-27
008 2020 bl uuuu u00u1 u #d
100 1 $aSILVA, C. de O. G.
245 $aCombination of MALDI-TOF MS and UHPLC-ESI-MS for the characterization of lytic polysaccharide monooxygenase activity.$h[electronic resource]
260 $c2020
520 $aLytic polysaccharide monooxygenases (LPMOs) are redox enzymes of high biotechnological interest due to their capacity to degrade recalcitrant polysaccharides, such as cellulose, by an oxidative mechanism. The characterization of LPMOs is challenging since they generate a variety of catalytic products which include native oligosaccharides (non-oxidized) and oligosaccharides oxidized at the reducing end (C1 position), the non-reducing end (C4 position), or both ends, with different degrees of polymerization. Moreover, oxidized products exist in equilibrium with their hydrated forms, which further complicates their identification. The lack of commercial analytical standards for all these possible forms of oxidized oligosaccharides and the low concentration of these products make LPMO functional characterization dependent on advanced mass spectrometry techniques capable of identifying the profile of oxidized products. Here, a new approach for the characterization of cellulose-active LPMOs based on the combination of MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS was proposed and optimized. The LPMO TrAA9A from Trichoderma reesei was used as a model enzyme to develop and test the MS methods. MALDI-TOF MS and UHPLC-ESI-MS methods were both capable of identifying putative C1, C4 and C1/C4 oxidized cello-oligosaccharides as well as their native counterparts generated after cellulose treatment with LPMO, which allows their utilization to characterize type I (C1-oxidizer), type II (C4-oxidizer) and type III (C1- and C4-oxidizer) LPMOs. Moreover, both methods were complementary since MALDI-TOF MS was capable of detecting oligosaccharides with higher degrees of polymerization (DP3?DP10), while UHPLC-ESI-MS allowed the evaluation of smaller oligosaccharides (DP1?DP5). The combined use of both methods offers a comprehensive description of LPMO catalytic products.
653 $aCaracterização de LPMOs
700 1 $aTEIXEIRA, S. T.
700 1 $aRODRIGUES, K. B.
700 1 $aSOUZA, A. A.
700 1 $aMONCLARO, A. V.
700 1 $aMENDES, T. D.
700 1 $aRIBEIRO, J. A. de A.
700 1 $aSIQUEIRA, F. G. de
700 1 $aFAVARO, L. C. de L.
700 1 $aABDELNUR, P. V.
773 $tAnalytical Methods$gn. 2, 2020.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
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Registro |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Clima Temperado. Para informações adicionais entre em contato com cpact.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Clima Temperado. |
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Data corrente: |
21/03/2023 |
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Data da última atualização: |
21/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
BICCA, J. M.; ARDUIN, R. L. N.; PINTO, L. F. S.; BAMBERG, A. L.; MIGUEL, P.; STUMPF, L. |
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Afiliação: |
JULIANA MACIEL BICCA; RICARDO LUIZ NUNES ARDUIN; LUIZ FERNANDO SPINELLI PINTO; ADILSON LUIS BAMBERG, CPACT; PABLO MIGUEL; LIZETE STUMPF. |
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Título: |
Clay stabilization and recovery of soil functions of a degraded solodic planosol through incorporation of agrominerals: A case study in southern Brazil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Journal of South American Earth Sciences, v. 122, 104177, 2023. |
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DOI: |
https://doi.org/10.1016/j.jsames.2022.104177 |
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Idioma: |
Inglês |
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Conteúdo: |
This paper describes the performance of addition rates of basaltic breccia and agricultural gypsum on clay stabilization and nutrients release of a solodic Planosol from a degraded borrow area located in Southern Brazil. A leaching experiment was carried out in PVC cylinders filled with 1.5 kg of soil (C horizon), in which two chemical amendments were added in three different proportions: a) basaltic breccia in 7, 14 and 21 Mg ha?1 doses; b) agricultural gypsum in 7, 14 and 21 Mg ha?1 doses; and c) a standard soil, without chemical correction. Distilled water (300 mL) were added every 7 days for 20 weeks and the pH, electrical conductivity, calcium, magnesium, sodium and potassium contents and the water-dispersible clay were analyzed in the collected leachates. Gypsum was more efficient than basaltic breccia to promote clay flocculation, even at the lower rate (7 Mg ha?1). The application of basaltic breccia up to 21 Mg ha?1 reduced the amount of nutrients released directly to the soil solution, reducing soil leaching. The application of basaltic breccia at the dosage of 7 Mg ha?1 was able to reduce more than 65% of the water-dispersive clay from the degraded solodic Planosol. |
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Palavras-Chave: |
Agromineral; Brecha basáltica; Gesso agrícola. |
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Thesagro: |
Corretivo; Gesso; Matéria Prima Mineral; Mineral; Solo. |
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Thesaurus NAL: |
Basaltic soils; Gypsum. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02152naa a2200313 a 4500
001 2152575
005 2023-03-21
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.jsames.2022.104177$2DOI
100 1 $aBICCA, J. M.
245 $aClay stabilization and recovery of soil functions of a degraded solodic planosol through incorporation of agrominerals$bA case study in southern Brazil.$h[electronic resource]
260 $c2023
520 $aThis paper describes the performance of addition rates of basaltic breccia and agricultural gypsum on clay stabilization and nutrients release of a solodic Planosol from a degraded borrow area located in Southern Brazil. A leaching experiment was carried out in PVC cylinders filled with 1.5 kg of soil (C horizon), in which two chemical amendments were added in three different proportions: a) basaltic breccia in 7, 14 and 21 Mg ha?1 doses; b) agricultural gypsum in 7, 14 and 21 Mg ha?1 doses; and c) a standard soil, without chemical correction. Distilled water (300 mL) were added every 7 days for 20 weeks and the pH, electrical conductivity, calcium, magnesium, sodium and potassium contents and the water-dispersible clay were analyzed in the collected leachates. Gypsum was more efficient than basaltic breccia to promote clay flocculation, even at the lower rate (7 Mg ha?1). The application of basaltic breccia up to 21 Mg ha?1 reduced the amount of nutrients released directly to the soil solution, reducing soil leaching. The application of basaltic breccia at the dosage of 7 Mg ha?1 was able to reduce more than 65% of the water-dispersive clay from the degraded solodic Planosol.
650 $aBasaltic soils
650 $aGypsum
650 $aCorretivo
650 $aGesso
650 $aMatéria Prima Mineral
650 $aMineral
650 $aSolo
653 $aAgromineral
653 $aBrecha basáltica
653 $aGesso agrícola
700 1 $aARDUIN, R. L. N.
700 1 $aPINTO, L. F. S.
700 1 $aBAMBERG, A. L.
700 1 $aMIGUEL, P.
700 1 $aSTUMPF, L.
773 $tJournal of South American Earth Sciences$gv. 122, 104177, 2023.
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