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| Registros recuperados : 27 | |
| Registros recuperados : 27 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
22/03/2010 |
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Data da última atualização: |
20/11/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
FRANÇA, L. V. de; NASCIMENTO, W. M.; CARMONA, R.; FREITAS, R. A. de. |
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Afiliação: |
Leomara Vieira de França, Universidade Federal de Pelotas; WARLEY MARCOS NASCIMENTO, CNPH; Ricardo Carmona, Universidade de Brasília; RAQUEL ALVES DE FREITAS, SIN. |
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Título: |
Viability of eggplant pollen. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, Londrina, v. 9, p. 320-327, 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to test methodologies to evaluate the pollen viability of eggplant. The pollen germination was tested in vitro, incubating the pollen during 0.5 and 16 hours in different sucrose concentrations (0; 2.5; 5.0; 7.5 and 10.0 g L -1). For the in vivo germination test, the pollen was placed during 4 and 24 hours in the flower stigma and after that stained with aniline blue and evaluated in an ultraviolet light microscope. The viability of the pollen grains was also evaluated in tetrazolium solutions (at the concentrations of 1%; 0.75% and 0.5%) and the staining was evaluated after 24 hours. The highest in vitro pollen germination was 10.8%, showing the necessity for a better calibration of the culture medium. The in vivo pollen germination was 66%, showing that this is an adequate methodology to estimate pollen viability in eggplant. The methodologies involving tetrazolium solution weren’t efficient to estimate pollen viability evaluation, because of the deficient pollen staining. |
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Palavras-Chave: |
Cultivo in vitro; Cultivo in vivo; Germinação in vitro; Germinação in vivo; Tetrazólio; Variabilidade polínica em berinjela. |
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Thesagro: |
Berinjela; Germinação; Pólen; Semente; Solanum Melongena. |
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Thesaurus NAL: |
Pollen germination; Tetrazolium. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/17667/1/franca-viability.pdf
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Marc: |
LEADER 01855naa a2200313 a 4500
001 1780407
005 2018-11-20
008 2009 bl uuuu u00u1 u #d
100 1 $aFRANÇA, L. V. de
245 $aViability of eggplant pollen.
260 $c2009
520 $aThis study aimed to test methodologies to evaluate the pollen viability of eggplant. The pollen germination was tested in vitro, incubating the pollen during 0.5 and 16 hours in different sucrose concentrations (0; 2.5; 5.0; 7.5 and 10.0 g L -1). For the in vivo germination test, the pollen was placed during 4 and 24 hours in the flower stigma and after that stained with aniline blue and evaluated in an ultraviolet light microscope. The viability of the pollen grains was also evaluated in tetrazolium solutions (at the concentrations of 1%; 0.75% and 0.5%) and the staining was evaluated after 24 hours. The highest in vitro pollen germination was 10.8%, showing the necessity for a better calibration of the culture medium. The in vivo pollen germination was 66%, showing that this is an adequate methodology to estimate pollen viability in eggplant. The methodologies involving tetrazolium solution weren’t efficient to estimate pollen viability evaluation, because of the deficient pollen staining.
650 $aPollen germination
650 $aTetrazolium
650 $aBerinjela
650 $aGerminação
650 $aPólen
650 $aSemente
650 $aSolanum Melongena
653 $aCultivo in vitro
653 $aCultivo in vivo
653 $aGerminação in vitro
653 $aGerminação in vivo
653 $aTetrazólio
653 $aVariabilidade polínica em berinjela
700 1 $aNASCIMENTO, W. M.
700 1 $aCARMONA, R.
700 1 $aFREITAS, R. A. de
773 $tCrop Breeding and Applied Biotechnology, Londrina$gv. 9, p. 320-327, 2009.
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Registro original: |
Embrapa Hortaliças (CNPH) |
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