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| Registros recuperados : 16 | |
| 3. |  | SOUSA, R. de C. P. de; SMIDERLE, O. J.; CHAGAS, E. A.; MELO FILHO, A. A. de. Período e dose mínima inter-relacionados a sanidade e comportamento fisiológico de leguminosa tratada com composto biotecnológico de sementes bioprocessadas do Caçari. In: SEMANA NACIONAL DE CIÊNCIA E TECNOLOGIA NO ESTADO DE RORAIMA, SNCT-RR , 12., 2017, Boa Vista. Boletim de Resumos... Boa Vista, RR: UERR/NUPECEM/LABTEMA, 2017.| Biblioteca(s): Embrapa Roraima. |
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| 7. | | MALDONADO, S. A. S.; CORDEIRO, A. C. C.; MELO, V. F.; SIQUEIRA, R. H. da S.; FERNÁNDEZ, I. M.; UCHÔA, S. C. P.; MELO FILHO, A. A. de. Biological Attributes of the Soil with Intercaled Crops and Oil Palm (Elaeis guineensis Jacq.): Sustainable Management in the Amazon. International Journal of Plant & Soil Science, v.26, n.5, p. 1-17, 2018.| Biblioteca(s): Embrapa Roraima. |
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| 10. | | FERNANDEZ, I. M.; CHAGAS, E. A.; MELO FILHO, A. A. de; MALDONADO, S. A. S.; SANTOS, R. C. dos; RIBEIRO, P. R. E.; CHAGAS, P. C.; MELO, A. C. G. R. de. Characterization of Bioactive Compounds in Northern Amazon Fruits. Journal of Agricultural Science, v. 11, n. 9, 2019| Biblioteca(s): Embrapa Roraima. |
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| 11. | | FERNANDEZ, I. M.; MELO FILHO, A. A. de; CHAGAS, E. A.; MALDONADO, S.; SANTOS, R. C. dos; RIVEIRO, P. R. E.; MELO, A. C. G. R. de; COSTA, H. N. R. da; MEDEIROS, S. R. Determinação de compostos fenólicos totais em frutas Amazônicas. In: CONGRESSO BRASILEIRO DE QUÍMICA, 56., 2016, Belém, PA. Anais... Rio de Janeiro: ABQ, 2016.| Biblioteca(s): Embrapa Roraima. |
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| 12. | | SANTOS, R. C. dos; MELO FILHO, A. A. de; CHAGAS, E. A.; TAKAHASHI, J. A.; FERRAZ, V. P.; COSTA, A. K. P.; MELO, A. C. G. R. de; MONTERO, I. F.; RIBEIRO, P. R. E. Fatty acid profile and bioactivity from Annona hypoglauca seeds oil. African journal of Biotechnology, v. 14, n. 30, p. 2377-2382, 2015.| Biblioteca(s): Embrapa Roraima. |
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| 13. | | SANTOS, R. C.; MELO FILHO, A. A. de; COSTA, H. N. R. da; PANERO, F. dos S.; BARRETO, H. C. dos S.; COSTA, M. C. G.; TONINI, H.; SOUSA, R. de C. P. de. Influência da adubação NPK no rendimento do óleo essencial de Eucalyptus camaldulensis. Orbital, v.2, n. 4, oct./dec., 2010.| Biblioteca(s): Embrapa Roraima. |
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| 14. | | MELO FILHO, A. A. de; FERNANDEZ, I. M.; CHAGAS, E. A.; SANTOS, R. C. dos; MELO, A. C. G. R. de; RIBEIRO, P. R. E.; TAKAHASSHIC, J. A.; MALDONADO, S.; COSTA, H. N. R. da. Physicochemical properties of the oil from murumuruí fruit (Astrocaryum acaule): reason w-6/w-3 and w-9 potencial. In: CONGRESSO BRASILEIRO DE QUÍMICA, 56., 2016, Belém, PA. Anais... Rio de Janeiro: ABQ, 2016.| Biblioteca(s): Embrapa Roraima. |
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| 15. | | FILHO, D.; MELO FILHO, A. A. de; CHAGAS, E. A.; SOUSA, R. de C. P. de; COSTA, A. K. P.; SANTOS, R. C. dos; MONTERO, I. F.; MELO, A. C. G. R. de; RIBEIRO, P. R. E.; MOZOMBITE, D. M. S.; YANES, C. V. I. Análise de minerais no óleo de camu-camu por ICP-OES. In: SEMINÁRIO INTERNACIONAL PROCESSAMENTO DE ÓLEOS E GORDURAS TENDÊNCIAS E DESAFIOS. Resumos... Florianópolis, SC: 2015.| Biblioteca(s): Embrapa Roraima. |
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| 16. | | FILHO, D.; MELO FILHO, A. A. de; CHAGAS, E. A.; SOUSA, R. de C. P. de; COSTA, A. K. P.; SANTOS, R. C. dos; MONTERO, I. F.; MELO, A. C. G. R. de; RIBEIRO, P. R. E.; MOZOMBITE, D. M. S.; YANES, C. V. I. Propriedades físico-químicas do óleo de camu-camu por RMN de 1H. In: SEMINÁRIO INTERNACIONAL PROCESSAMENTO DE ÓLEOS E GORDURAS TENDÊNCIAS E DESAFIOS. Resumos... Florianópolis, SC: 2015.| Biblioteca(s): Embrapa Roraima. |
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| Registros recuperados : 16 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
10/11/2017 |
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Data da última atualização: |
13/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
GALVÃO, C. E.; FRAGOSO, S. P.; OLIVEIRA, C. E. de; FORNER, O.; PEREIRA, R. R. B.; SOARES, C. O.; ROSINHA, G. M. S. |
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Afiliação: |
CLEBER EDUARDO GALVÃO, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS.; STENIO PERDIGÃO FRAGOSO, Carlos Chagas Institute, Oswaldo Cruz Foundation (ICC-FIOCRUZ), Curitiba, PR.; CARINA ELISEI DE OLIVEIRA, Dom Bosco Catholic University (UCDB), Campo Grande, MS.; ODINÉIA FORNER, Federal University of Paraná (UFPR), Curitiba, PR.; RENATA RIBEIRO BASTOS PEREIRA, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS.; CLEBER OLIVEIRA SOARES, DE/TT; GRACIA MARIA SOARES ROSINHA, CNPGC. |
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Título: |
Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
BMC Microbiology, v. 17, n. 202, p. 1-8, sept., 2017. |
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DOI: |
10.1186/s12866-017-1110-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Caseous lymphadenitis (CLA) is a disease that affects sheep, goats and occasionally humans. The etiologic agent is the Corynebacterium pseudotuberculosis bacillus. The objective of this study was to build a gene expression library from C. pseudotuberculosis and use immunoscreening to identify genes that encode potential antigenic proteins for the development of DNA and subunit vaccines against CLA. Results: A wild strain of C. pseudotuberculosis was used for extraction and partial digestion of genomic DNA. Sequences between 1000 and 5000 base pairs (bp) were excised from the gel, purified, and the digested DNA fragments were joined to bacteriophage vector ZAP Express, packaged into phage and transfected into Escherichia coli . For immunoscreening a positive sheep sera pool and a negative sera pool for CLA were used. Four clones were identified that strongly reacted to sera. The clones were confirmed by polymerase chain reaction (PCR) followed by sequencing for genomic comparison of C. pseudotuberculosis in GenBank. The genes identified were dak2, fagA, fagB, NlpC/P60 protein family and LPxTG putative protein family. Conclusion: Proteins of this type can be antigenic which could aid in the development of subunit or DNA vaccines against CLA as well as in the development of serological tests for diagnosis. Immunoscreening of the gene expression library was shown to be a sensitive and efficient technique to identify probable immunodominant genes. |
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Palavras-Chave: |
DNA vaccines. |
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Thesagro: |
Corynebacterium Pseudotuberculosis. |
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Thesaurus NAL: |
Caseous lymphadenitis; Gene expression; Sheep. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166571/1/Identification-of-new-Corynebacterium.pdf
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Marc: |
LEADER 02301naa a2200265 a 4500
001 2079448
005 2017-11-13
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1186/s12866-017-1110-7$2DOI
100 1 $aGALVÃO, C. E.
245 $aIdentification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library.$h[electronic resource]
260 $c2017
520 $aBackground: Caseous lymphadenitis (CLA) is a disease that affects sheep, goats and occasionally humans. The etiologic agent is the Corynebacterium pseudotuberculosis bacillus. The objective of this study was to build a gene expression library from C. pseudotuberculosis and use immunoscreening to identify genes that encode potential antigenic proteins for the development of DNA and subunit vaccines against CLA. Results: A wild strain of C. pseudotuberculosis was used for extraction and partial digestion of genomic DNA. Sequences between 1000 and 5000 base pairs (bp) were excised from the gel, purified, and the digested DNA fragments were joined to bacteriophage vector ZAP Express, packaged into phage and transfected into Escherichia coli . For immunoscreening a positive sheep sera pool and a negative sera pool for CLA were used. Four clones were identified that strongly reacted to sera. The clones were confirmed by polymerase chain reaction (PCR) followed by sequencing for genomic comparison of C. pseudotuberculosis in GenBank. The genes identified were dak2, fagA, fagB, NlpC/P60 protein family and LPxTG putative protein family. Conclusion: Proteins of this type can be antigenic which could aid in the development of subunit or DNA vaccines against CLA as well as in the development of serological tests for diagnosis. Immunoscreening of the gene expression library was shown to be a sensitive and efficient technique to identify probable immunodominant genes.
650 $aCaseous lymphadenitis
650 $aGene expression
650 $aSheep
650 $aCorynebacterium Pseudotuberculosis
653 $aDNA vaccines
700 1 $aFRAGOSO, S. P.
700 1 $aOLIVEIRA, C. E. de
700 1 $aFORNER, O.
700 1 $aPEREIRA, R. R. B.
700 1 $aSOARES, C. O.
700 1 $aROSINHA, G. M. S.
773 $tBMC Microbiology$gv. 17, n. 202, p. 1-8, sept., 2017.
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