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Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
21/07/2021 |
Data da última atualização: |
21/07/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SILVA, C. M. de J.; DIAS, R. de C. S.; MELO, N. F. de. |
Afiliação: |
CARLA MARIA DE JESUS SILVA; RITA DE CASSIA SOUZA DIAS, CPATSA; NATONIEL FRANKLIN DE MELO, CPATSA. |
Título: |
The effect of temperature and growth regulators on callus induction in watermelon anthers. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Brazilian Archives of Biology and Technology, v. 64, e21180505, 2021. |
ISSN: |
1678-4324 |
DOI: |
https://doi.org/10.1590/1678-4324-2021180505 |
Idioma: |
Inglês |
Conteúdo: |
Callus induction is one of the pathways required for haploid plant regeneration through anther culture. Pollen viability, as well as the effect of growth regulators and cold pretreatment on anthers of two watermelon lines (Smile and Sugar Baby) to induce callus formation were herein evaluated. Pollen viability was estimated through the staining technique using 2% acetic carmine. Male flower buds were collected and disinfested to allow removal anthers. These anthers were placed on Murashige and Skoog medium, which was supplemented with 2,4-dichlorophenoxyacetic (2,4-D) at 0.0, 0.5, 1.0, 2.0 or 5.0 ?M or with 6-benzylaminopurine at 0.0, 0.5, 1.0, 1.5, or 2.0 ?M, in combination with 2.0 ?M of 2,4-dichlorophenoxyacetic. Anthers were pretreated at 4 °C, for two days and then placed in vitro. Both watermelon lines provided high pollen viability rates (from 93 to 98%). The 2.0 and 5.0 ?M concentrations of 2,4-D stimulated higher friable callus formation. The optimal concentration of 2,4-D was estimated at 3.78 ?M and 4.17 ?M, which had callus induction rates of 64% and 52%, respectively. The combination of 2.0 ?M of 2,4-D and 6-benzylaminopurine did not lead to increased anther response to callus induction. The pre-treatment applied to flower buds at 4 °C enabled callus induction and the anther response to callus induction was genotype-dependent. |
Palavras-Chave: |
Calogênese de anteras; Viabilidade do pólen. |
Thesagro: |
Citrullus Lanatus; Cucurbitaceae; Fruta Cucurbitácea; Melancia; Regulador de Crescimento. |
Thesaurus Nal: |
Growth regulators; Watermelons. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/224590/1/The-effect-of-temperature-and-growth-regulator-2021.pdf
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Marc: |
LEADER 02236naa a2200277 a 4500 001 2133062 005 2021-07-21 008 2021 bl uuuu u00u1 u #d 022 $a1678-4324 024 7 $ahttps://doi.org/10.1590/1678-4324-2021180505$2DOI 100 1 $aSILVA, C. M. de J. 245 $aThe effect of temperature and growth regulators on callus induction in watermelon anthers.$h[electronic resource] 260 $c2021 520 $aCallus induction is one of the pathways required for haploid plant regeneration through anther culture. Pollen viability, as well as the effect of growth regulators and cold pretreatment on anthers of two watermelon lines (Smile and Sugar Baby) to induce callus formation were herein evaluated. Pollen viability was estimated through the staining technique using 2% acetic carmine. Male flower buds were collected and disinfested to allow removal anthers. These anthers were placed on Murashige and Skoog medium, which was supplemented with 2,4-dichlorophenoxyacetic (2,4-D) at 0.0, 0.5, 1.0, 2.0 or 5.0 ?M or with 6-benzylaminopurine at 0.0, 0.5, 1.0, 1.5, or 2.0 ?M, in combination with 2.0 ?M of 2,4-dichlorophenoxyacetic. Anthers were pretreated at 4 °C, for two days and then placed in vitro. Both watermelon lines provided high pollen viability rates (from 93 to 98%). The 2.0 and 5.0 ?M concentrations of 2,4-D stimulated higher friable callus formation. The optimal concentration of 2,4-D was estimated at 3.78 ?M and 4.17 ?M, which had callus induction rates of 64% and 52%, respectively. The combination of 2.0 ?M of 2,4-D and 6-benzylaminopurine did not lead to increased anther response to callus induction. The pre-treatment applied to flower buds at 4 °C enabled callus induction and the anther response to callus induction was genotype-dependent. 650 $aGrowth regulators 650 $aWatermelons 650 $aCitrullus Lanatus 650 $aCucurbitaceae 650 $aFruta Cucurbitácea 650 $aMelancia 650 $aRegulador de Crescimento 653 $aCalogênese de anteras 653 $aViabilidade do pólen 700 1 $aDIAS, R. de C. S. 700 1 $aMELO, N. F. de 773 $tBrazilian Archives of Biology and Technology$gv. 64, e21180505, 2021.
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Embrapa Semiárido (CPATSA) |
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102. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FARI, M.; SORVARI, S.; AHANEN, K; TOLDI, O.; MELO, N. F. de; HIRSALMI, H. Development and germination of somatic embryos on sugar-free wheat starch medium in carrot (Daucus carota L. cv. Nantes Duke). In: ENCONTRO BRASILEIRO DE BIOTECNOLOGIA VEGETAL, 2., 1997, Gramado. Programas e resumos... Passo Fundo: REDBIO Sub-Região Brasil, 1997. p. 128.Tipo: Resumo em Anais de Congresso |
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