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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
30/07/2013 |
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Data da última atualização: |
30/07/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PAULO, E. M.; VASCONCELOS, M. P.; OLIVEIRA, I. S.; AFFE, H. M. J.; NASCIMENTO, R. dos S.; MELO, I. S. de; ROQUE, M. R. de A.; ASSIS, S. A. de. |
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Afiliação: |
ELINALVA MACIEL PAULO, UEFS; MURILO PINHO VASCONCELOS, UEFS; IVELISE SANTIAGO OLIVEIRA, UEFS; HELEN MICHELLE DE JESUS AFFE, UEFS; ROSELY DOS SANTOS NASCIMENTO, CNPMA; ITAMAR SOARES DE MELO, CNPMA; MILTON RICARDO DE ABREU ROQUE, UFBA; SANDRA APARECIDA DE ASSIS, UEFS. |
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Título: |
An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Ciência e Tecnologia de Alimentos, Campinas, v. 32, n. 4, p. 710-714, 2012. |
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Idioma: |
Português |
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Conteúdo: |
Abstract: The accumulation of exopolysaccharides (EPS) produced by microorganisms occurs in the presence of excess substrate and limiting conditions of elements that are essential to growth, such as nitrogen, phosphorus, sulfur, and magnesium. The presence of EPS produced by bacterial cells contributes to slime colonies formation in solid medium and increased viscosity in liquid medium. This paper proposes an alternative method for screening EPS-producing lactic acid bacteria using solid medium-containing discs of filter paper that are saturated with active cultures. The screening was carried out under different culture conditions varying the type of sugar, pH, and temperature. EPS production was visualized by the presence of mucoid colonies on the discs, which was confirmed by the formation of a precipitate when part of this colony was mixed with absolute alcohol. The established conditions for obtaining a high number of isolates producing EPS were 10% sucrose, pH 7.5 and 28 °C. This method proved to be effective and economical because several strains could be tested on the same plate, with immediate confirmation. Resumo: Em geral, o acúmulo de exopolissacarídeos (EPS) produzidos pelos microrganismos ocorre na presença de excesso de substrato e condições limitantes de elementos essenciais ao crescimento, como nitrogênio, fósforo, enxofre, ou magnésio. A presença de EPS associada com células bacterianas é reconhecida em meio sólido para a formação de colônias mucoides e no meio líquido pelo aumento da viscosidade. Este trabalho propõe um método alternativo para realizar triagem de bactérias láticas produtoras de EPS utilizando meio sólido contendo discos de papel de filtro impregnado pela cultura. A seleção foi realizada em diferentes condições de cultivo, variando o tipo de açúcar, pH e temperatura. A produção de EPS foi visualizada pela presença da colônia mucoide sobre os discos, que foram confirmadas pelo precipitado, quando parte desta colônia foi misturada com álcool absoluto. As condições de cultivo em que houve maior produção de EPS foram: sacarose 10%, pH 7,5 a 28 °C. Este método provou ser eficaz e econômico, uma vez que várias cepas podem ser testadas na mesma placa, sendo possível realizar uma prévia confirmação da produção do EPS. MenosAbstract: The accumulation of exopolysaccharides (EPS) produced by microorganisms occurs in the presence of excess substrate and limiting conditions of elements that are essential to growth, such as nitrogen, phosphorus, sulfur, and magnesium. The presence of EPS produced by bacterial cells contributes to slime colonies formation in solid medium and increased viscosity in liquid medium. This paper proposes an alternative method for screening EPS-producing lactic acid bacteria using solid medium-containing discs of filter paper that are saturated with active cultures. The screening was carried out under different culture conditions varying the type of sugar, pH, and temperature. EPS production was visualized by the presence of mucoid colonies on the discs, which was confirmed by the formation of a precipitate when part of this colony was mixed with absolute alcohol. The established conditions for obtaining a high number of isolates producing EPS were 10% sucrose, pH 7.5 and 28 °C. This method proved to be effective and economical because several strains could be tested on the same plate, with immediate confirmation. Resumo: Em geral, o acúmulo de exopolissacarídeos (EPS) produzidos pelos microrganismos ocorre na presença de excesso de substrato e condições limitantes de elementos essenciais ao crescimento, como nitrogênio, fósforo, enxofre, ou magnésio. A presença de EPS associada com células bacterianas é reconhecida em meio sólido para a formação de colônias mucoides e no m... Mostrar Tudo |
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Palavras-Chave: |
Exopolissacarídeos; Triagem. |
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Thesagro: |
Análise de laboratório; Polissacarídeo. |
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Thesaurus Nal: |
Exopolysaccharides; Lactic acid bacteria; Screening. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/86784/1/2013AP04.pdf
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Marc: |
LEADER 03240naa a2200289 a 4500
001 1963060
005 2013-07-30
008 2012 bl uuuu u00u1 u #d
100 1 $aPAULO, E. M.
245 $aAn alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation.$h[electronic resource]
260 $c2012
520 $aAbstract: The accumulation of exopolysaccharides (EPS) produced by microorganisms occurs in the presence of excess substrate and limiting conditions of elements that are essential to growth, such as nitrogen, phosphorus, sulfur, and magnesium. The presence of EPS produced by bacterial cells contributes to slime colonies formation in solid medium and increased viscosity in liquid medium. This paper proposes an alternative method for screening EPS-producing lactic acid bacteria using solid medium-containing discs of filter paper that are saturated with active cultures. The screening was carried out under different culture conditions varying the type of sugar, pH, and temperature. EPS production was visualized by the presence of mucoid colonies on the discs, which was confirmed by the formation of a precipitate when part of this colony was mixed with absolute alcohol. The established conditions for obtaining a high number of isolates producing EPS were 10% sucrose, pH 7.5 and 28 °C. This method proved to be effective and economical because several strains could be tested on the same plate, with immediate confirmation. Resumo: Em geral, o acúmulo de exopolissacarídeos (EPS) produzidos pelos microrganismos ocorre na presença de excesso de substrato e condições limitantes de elementos essenciais ao crescimento, como nitrogênio, fósforo, enxofre, ou magnésio. A presença de EPS associada com células bacterianas é reconhecida em meio sólido para a formação de colônias mucoides e no meio líquido pelo aumento da viscosidade. Este trabalho propõe um método alternativo para realizar triagem de bactérias láticas produtoras de EPS utilizando meio sólido contendo discos de papel de filtro impregnado pela cultura. A seleção foi realizada em diferentes condições de cultivo, variando o tipo de açúcar, pH e temperatura. A produção de EPS foi visualizada pela presença da colônia mucoide sobre os discos, que foram confirmadas pelo precipitado, quando parte desta colônia foi misturada com álcool absoluto. As condições de cultivo em que houve maior produção de EPS foram: sacarose 10%, pH 7,5 a 28 °C. Este método provou ser eficaz e econômico, uma vez que várias cepas podem ser testadas na mesma placa, sendo possível realizar uma prévia confirmação da produção do EPS.
650 $aExopolysaccharides
650 $aLactic acid bacteria
650 $aScreening
650 $aAnálise de laboratório
650 $aPolissacarídeo
653 $aExopolissacarídeos
653 $aTriagem
700 1 $aVASCONCELOS, M. P.
700 1 $aOLIVEIRA, I. S.
700 1 $aAFFE, H. M. J.
700 1 $aNASCIMENTO, R. dos S.
700 1 $aMELO, I. S. de
700 1 $aROQUE, M. R. de A.
700 1 $aASSIS, S. A. de
773 $tCiência e Tecnologia de Alimentos, Campinas$gv. 32, n. 4, p. 710-714, 2012.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
29/08/2023 |
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Data da última atualização: |
29/08/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CHOCOBAR, M. L. E.; BELLO, H. J. S.; BASSETTO, C. C.; SILVA-MARINHO, N. M.; SATO, L. M. N.; SPERB, C.; CHAGAS, A. C. de S.; AMARANTE, A. F. T. |
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Afiliação: |
M. L. E. CHOCOBAR, São Paulo State University; H. J. S. BELLO, São Paulo State University; C. C. BASSETTO, São Paulo State University; N. M. SILVA-MARINHO, São Paulo State University; L. M. N. SATO, São Paulo State University; C. SPERB, São Paulo State University; ANA CAROLINA DE SOUZA CHAGAS, CPPSE; A. F. T. AMARANTE, São Paulo State University. |
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Título: |
Biotic potential and pathogenicity of a Haemonchus contortus susceptible laboratory isolate compared to a resistant field isolate in Brazil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Small Ruminant Research, v. 227, oct. 2023, 107063. |
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Páginas: |
10 p. |
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DOI: |
https://doi.org/10.1016/j.smallrumres.2023.107063 |
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Idioma: |
Inglês |
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Conteúdo: |
The high prevalence of anthelmintic resistance of Haemonchus contortus is threatening sheep farming. In this context, resistant populations? replacement using susceptible isolates appears to be a strategy to overcome this situation. The aim of the present study was to evaluate the ability of a susceptible H. contortus laboratory isolate in comparison with a multiresistant field isolate to infect lambs and to study its behaviour in field conditions. Sixteen worm-free lambs were experimentally infected with 4000 infective larvae (L3) of a H. contortus anthelmintic-resistant field isolate (Group R, n = 8) or a laboratory isolate susceptible to anthelmintics (Group S, n = 8). Groups were placed in separate clean pastures, where they were kept for 266 days. Faecal egg counts (FEC) showed maximum values between 21- and 42-days post-infection (PI) and decreased over time, with no statistical differences between groups (P > 0.05). The pathogenicity of the isolates was similar, as lambs from both groups showed a decrease in packed cell volume and total plasma protein values in the beginning of the study, coinciding with the highest FECs. Production of L3 were similar for both groups, when evaluating faecal cultures kept in vitro or L3 recovered from pasture samples and from faecal pats collected from the paddocks, which suggests that both isolates have the same potential of development in vitro and in the environment. The degree of resistance/susceptibility to thiabendazole (TIA), levamisole (LEV), monepantel (MON) and ivermectin (IVE) was evaluated using the Larval Development Assay (RESISTA-Test©) 160 days PI. The resistant isolate showed a high resistance factor (RF) to TIA (141), LEV (822), MON (34) and IVE (196), while the RF of the susceptible isolate remained below three, which indicates high susceptibility to all drugs tested. Our study demonstrates that the susceptible isolate was able to infect lambs and did not show higher pathogenicity nor any differences on its biotic potential when compared to the resistant field isolate. These results indicate that the susceptible isolate has the potential to be used to replace a resistant population of H. contortus, which should be validated in long-term studies under commercial sheep farming conditions. MenosThe high prevalence of anthelmintic resistance of Haemonchus contortus is threatening sheep farming. In this context, resistant populations? replacement using susceptible isolates appears to be a strategy to overcome this situation. The aim of the present study was to evaluate the ability of a susceptible H. contortus laboratory isolate in comparison with a multiresistant field isolate to infect lambs and to study its behaviour in field conditions. Sixteen worm-free lambs were experimentally infected with 4000 infective larvae (L3) of a H. contortus anthelmintic-resistant field isolate (Group R, n = 8) or a laboratory isolate susceptible to anthelmintics (Group S, n = 8). Groups were placed in separate clean pastures, where they were kept for 266 days. Faecal egg counts (FEC) showed maximum values between 21- and 42-days post-infection (PI) and decreased over time, with no statistical differences between groups (P > 0.05). The pathogenicity of the isolates was similar, as lambs from both groups showed a decrease in packed cell volume and total plasma protein values in the beginning of the study, coinciding with the highest FECs. Production of L3 were similar for both groups, when evaluating faecal cultures kept in vitro or L3 recovered from pasture samples and from faecal pats collected from the paddocks, which suggests that both isolates have the same potential of development in vitro and in the environment. The degree of resistance/susceptibility to thiabendazole (TIA), le... Mostrar Tudo |
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Palavras-Chave: |
Anthelmintic Resistance; Nematodes; Parasitic Control; Refugia. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 03176naa a2200277 a 4500
001 2156181
005 2023-08-29
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.smallrumres.2023.107063$2DOI
100 1 $aCHOCOBAR, M. L. E.
245 $aBiotic potential and pathogenicity of a Haemonchus contortus susceptible laboratory isolate compared to a resistant field isolate in Brazil.$h[electronic resource]
260 $c2023
300 $a10 p.
520 $aThe high prevalence of anthelmintic resistance of Haemonchus contortus is threatening sheep farming. In this context, resistant populations? replacement using susceptible isolates appears to be a strategy to overcome this situation. The aim of the present study was to evaluate the ability of a susceptible H. contortus laboratory isolate in comparison with a multiresistant field isolate to infect lambs and to study its behaviour in field conditions. Sixteen worm-free lambs were experimentally infected with 4000 infective larvae (L3) of a H. contortus anthelmintic-resistant field isolate (Group R, n = 8) or a laboratory isolate susceptible to anthelmintics (Group S, n = 8). Groups were placed in separate clean pastures, where they were kept for 266 days. Faecal egg counts (FEC) showed maximum values between 21- and 42-days post-infection (PI) and decreased over time, with no statistical differences between groups (P > 0.05). The pathogenicity of the isolates was similar, as lambs from both groups showed a decrease in packed cell volume and total plasma protein values in the beginning of the study, coinciding with the highest FECs. Production of L3 were similar for both groups, when evaluating faecal cultures kept in vitro or L3 recovered from pasture samples and from faecal pats collected from the paddocks, which suggests that both isolates have the same potential of development in vitro and in the environment. The degree of resistance/susceptibility to thiabendazole (TIA), levamisole (LEV), monepantel (MON) and ivermectin (IVE) was evaluated using the Larval Development Assay (RESISTA-Test©) 160 days PI. The resistant isolate showed a high resistance factor (RF) to TIA (141), LEV (822), MON (34) and IVE (196), while the RF of the susceptible isolate remained below three, which indicates high susceptibility to all drugs tested. Our study demonstrates that the susceptible isolate was able to infect lambs and did not show higher pathogenicity nor any differences on its biotic potential when compared to the resistant field isolate. These results indicate that the susceptible isolate has the potential to be used to replace a resistant population of H. contortus, which should be validated in long-term studies under commercial sheep farming conditions.
653 $aAnthelmintic Resistance
653 $aNematodes
653 $aParasitic Control
653 $aRefugia
700 1 $aBELLO, H. J. S.
700 1 $aBASSETTO, C. C.
700 1 $aSILVA-MARINHO, N. M.
700 1 $aSATO, L. M. N.
700 1 $aSPERB, C.
700 1 $aCHAGAS, A. C. de S.
700 1 $aAMARANTE, A. F. T.
773 $tSmall Ruminant Research$gv. 227, oct. 2023, 107063.
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