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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
17/11/2022 |
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Data da última atualização: |
14/07/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ZWAR, I. P.; TROTTA, C. do V.; ZIOTTI, A. B. S.; LIMA NETO, M.; ARAÚJO, W. L. de; MELO, I. S. de; OTTONI, C. A.; SOUZA, A. O. de. |
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Afiliação: |
INGRID PADOVESE ZWAR, INSTITUTO BUTANTAN; CATERINA DO VALLE TROTTA, UNIVERSIDADE ESTADUAL PAULISTA; ANA BEATRIZ SICCHIERI ZIOTTI, UNIVERSIDADE ESTADUAL PAULISTA; MILTON LIMA NETO, UNIVERSIDADE ESTADUAL PAULISTA; WELINGTON LUIZ DE ARAÚJO, UNIVERSIDADE DE SÃO PAULO; ITAMAR SOARES DE MELO, CNPMA; CRISTIANE ANGÉLICA OTTONI, UNIVERSIDADE ESTADUAL PAULISTA; ANA OLÍVIA DE SOUZA, INSTITUTO BUTANTAN. |
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Título: |
Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Journal of Basic Microbiology, v. 63, n. 1, p. 64-74, 2022. |
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ISSN: |
0233-111X |
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DOI: |
https://doi.org/10.1002/jobm.202200439 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: To find effective silver nanoparticles (AgNPs) for control of phytopathogens, in this study, two strains of actinomycetes isolated from the soil of the Brazilian biome Caatinga (Caat5-35) and from mangrove sediment (Canv1-58) were utilized. The strains were identified by using the 16S rRNA gene sequencing as Streptomyces sp., related to Streptomyces mimosus species. The obtained AgNPs were coded as AgNPs 35 and AgNPs58 and characterized by size and morphology using dynamic light scattering, zeta potential, transmission electron microscopy, and Fourier transformed infrared (FTIR). The antifungal activity of the AgNPs35 and AgNPs58 was evaluated in vitro by the minimal inhibitory concentration (MIC) assay on the phytopathogens, Alternaria solani, Alternaria alternata, and Colletotrichum gloeosporioides. The phytotoxic effect was evaluated by the germination rate and seedling growth of rice (Oryza sativa). AgNPs35 and AgNPs58 showed surface plasmon resonance and average sizes of 30 and 60 nm, respectively. Both AgNPs presented spherical shape and the FTIR analysis confirmed the presence of functional groups such as free amines and hydroxyls of biomolecules bounded to the external layer of the nanoparticles. Both AgNPs inhibited the growth of the three phytopathogens tested, and A. alternate was the most sensible (MIC < = 4 µM). Moreover, the AgNPs35 and AgNPs58 did not induce phytotoxic effects on the germination and development of rice seedlings. In conclusion, these AgNPs are promising candidates to biocontrol of these phytopathogens without endangering rice plants. MenosAbstract: To find effective silver nanoparticles (AgNPs) for control of phytopathogens, in this study, two strains of actinomycetes isolated from the soil of the Brazilian biome Caatinga (Caat5-35) and from mangrove sediment (Canv1-58) were utilized. The strains were identified by using the 16S rRNA gene sequencing as Streptomyces sp., related to Streptomyces mimosus species. The obtained AgNPs were coded as AgNPs 35 and AgNPs58 and characterized by size and morphology using dynamic light scattering, zeta potential, transmission electron microscopy, and Fourier transformed infrared (FTIR). The antifungal activity of the AgNPs35 and AgNPs58 was evaluated in vitro by the minimal inhibitory concentration (MIC) assay on the phytopathogens, Alternaria solani, Alternaria alternata, and Colletotrichum gloeosporioides. The phytotoxic effect was evaluated by the germination rate and seedling growth of rice (Oryza sativa). AgNPs35 and AgNPs58 showed surface plasmon resonance and average sizes of 30 and 60 nm, respectively. Both AgNPs presented spherical shape and the FTIR analysis confirmed the presence of functional groups such as free amines and hydroxyls of biomolecules bounded to the external layer of the nanoparticles. Both AgNPs inhibited the growth of the three phytopathogens tested, and A. alternate was the most sensible (MIC < = 4 µM). Moreover, the AgNPs35 and AgNPs58 did not induce phytotoxic effects on the germination and development of rice seedlings. In conclusion, these... Mostrar Tudo |
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Thesagro: |
Actinomiceto; Controle Biológico; Doença de Planta. |
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Thesaurus Nal: |
Actinomyces; Biological control agents; Fungal diseases of plants; Nanoparticles; Nanosilver. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02634naa a2200325 a 4500
001 2148405
005 2023-07-14
008 2022 bl uuuu u00u1 u #d
022 $a0233-111X
024 7 $ahttps://doi.org/10.1002/jobm.202200439$2DOI
100 1 $aZWAR, I. P.
245 $aBiosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens.$h[electronic resource]
260 $c2022
520 $aAbstract: To find effective silver nanoparticles (AgNPs) for control of phytopathogens, in this study, two strains of actinomycetes isolated from the soil of the Brazilian biome Caatinga (Caat5-35) and from mangrove sediment (Canv1-58) were utilized. The strains were identified by using the 16S rRNA gene sequencing as Streptomyces sp., related to Streptomyces mimosus species. The obtained AgNPs were coded as AgNPs 35 and AgNPs58 and characterized by size and morphology using dynamic light scattering, zeta potential, transmission electron microscopy, and Fourier transformed infrared (FTIR). The antifungal activity of the AgNPs35 and AgNPs58 was evaluated in vitro by the minimal inhibitory concentration (MIC) assay on the phytopathogens, Alternaria solani, Alternaria alternata, and Colletotrichum gloeosporioides. The phytotoxic effect was evaluated by the germination rate and seedling growth of rice (Oryza sativa). AgNPs35 and AgNPs58 showed surface plasmon resonance and average sizes of 30 and 60 nm, respectively. Both AgNPs presented spherical shape and the FTIR analysis confirmed the presence of functional groups such as free amines and hydroxyls of biomolecules bounded to the external layer of the nanoparticles. Both AgNPs inhibited the growth of the three phytopathogens tested, and A. alternate was the most sensible (MIC < = 4 µM). Moreover, the AgNPs35 and AgNPs58 did not induce phytotoxic effects on the germination and development of rice seedlings. In conclusion, these AgNPs are promising candidates to biocontrol of these phytopathogens without endangering rice plants.
650 $aActinomyces
650 $aBiological control agents
650 $aFungal diseases of plants
650 $aNanoparticles
650 $aNanosilver
650 $aActinomiceto
650 $aControle Biológico
650 $aDoença de Planta
700 1 $aTROTTA, C. do V.
700 1 $aZIOTTI, A. B. S.
700 1 $aLIMA NETO, M.
700 1 $aARAÚJO, W. L. de
700 1 $aMELO, I. S. de
700 1 $aOTTONI, C. A.
700 1 $aSOUZA, A. O. de
773 $tJournal of Basic Microbiology$gv. 63, n. 1, p. 64-74, 2022.
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Embrapa Meio Ambiente (CNPMA) |
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| Registros recuperados : 563 | |
| 2. |  | MELO, I. S. de. Bioremediation of pesticides in soils. In: INTERNATIONAL CONFERENCE, 2004, Buenos Aires, Argentina. Resumos...Monitoring systems and novel technologies for detection/removal of pollutants in/from ecosystems. Buenos Aires, Argentina, 2004. p.18-19. pág. 18-19| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 3. | | MELO, I. S. de. Biorremediação de áreas contaminadas. In: SEMINÁRIO SOBRE PERSPECTIVAS DA BIOTENOLOGIA EM PIRACICABA, 2001. Anais...Piracicaba: ESALQ- Escola Superior de Agricultura Luiz de Queiroz, p. 7-8, 2001| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| Registros recuperados : 563 | |
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