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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
18/05/2007 |
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Data da última atualização: |
08/06/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ZUCCHI, T. D.; ZUCCHI, F. D.; POLI, P.; MELO, I. S. de; ZUCCHI, T. M. A. D. |
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Afiliação: |
Tiago Domingues Zucchi, ICB-USP; Fernando Domingues Zucchi, ICB-USP; Paola Poli, Dipartimento di Genetica Antropologia Evoluzione, Universita di Parma; ITAMAR SOARES DE MELO, CNPMA; Tania M. A. D. Zucchi, ICB-USP. |
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Título: |
A short-term test adapted to detect the genotoxic effects of environmental volatile pollutants (benzene fumes) using the filamentous fungus Aspergillus nidulans. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Journal of Environmental Monitoring, Washington, DC, v. 7, n. 6, p. 598-602, 2005. |
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Idioma: |
Inglês |
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Conteúdo: |
With the recent focus on environmental problems, increasing awareness of the harmful effects of industrial and agricultural pollution has created a demand for progressively more sophisticated pollutant and toxicity detection methods. Using Aspergillus nidulans strains this work presents a new short term-test that, most importantly, enables the rapid and inexpensive detection of volatile pollutants that induce genotoxic/carcinogenic effects in animals. The main aim is to contribute to environmental health protection, and special attention is directed to monitoring the hazard posed by benzene (as a carcinogenic agent model) mainly because its ubiquitous presence often leads to severe noxious effects in humans among whom increased rates of human leukemia have been reported. To evaluate even the submutagenic effects of benzene fumes, two Aspergillus nidulans diploid strains, heterozygous for several auxotrophic mutations, were used. The DNA lesions produced stimulate mitotic recombination and homozygotization of auxotrophic recessive mutations. Conidial exposure to a saturated atmosphere of benzene fumes for 20 s was enough to increase the mitotic recombination frequencies significantly. Genetic analyses of treated diploids evidenced alterations related to mitotic recombination frequencies, gene expression, and allelic segregation rates. Altogether they reflect the potential of benzene to induce alterations in the fungal DNA, and albeit indirectly, they also respond for the genotoxic/carcinogenic harmful side effects widely connected to benzene. This is the first description of a sensitive, rapid and inexpensive test able to detect the submutagenic dose effects of volatile environmental compounds. In addition, despite concentrating on benzene the same test can be applied to many other pollutants, volatile or not. Additionally, the test can also be used to detect the antigenotoxic properties of foods and drugs. MenosWith the recent focus on environmental problems, increasing awareness of the harmful effects of industrial and agricultural pollution has created a demand for progressively more sophisticated pollutant and toxicity detection methods. Using Aspergillus nidulans strains this work presents a new short term-test that, most importantly, enables the rapid and inexpensive detection of volatile pollutants that induce genotoxic/carcinogenic effects in animals. The main aim is to contribute to environmental health protection, and special attention is directed to monitoring the hazard posed by benzene (as a carcinogenic agent model) mainly because its ubiquitous presence often leads to severe noxious effects in humans among whom increased rates of human leukemia have been reported. To evaluate even the submutagenic effects of benzene fumes, two Aspergillus nidulans diploid strains, heterozygous for several auxotrophic mutations, were used. The DNA lesions produced stimulate mitotic recombination and homozygotization of auxotrophic recessive mutations. Conidial exposure to a saturated atmosphere of benzene fumes for 20 s was enough to increase the mitotic recombination frequencies significantly. Genetic analyses of treated diploids evidenced alterations related to mitotic recombination frequencies, gene expression, and allelic segregation rates. Altogether they reflect the potential of benzene to induce alterations in the fungal DNA, and albeit indirectly, they also respond for the geno... Mostrar Tudo |
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Palavras-Chave: |
Microbiologia ambiental. |
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Thesagro: |
Aspergillus Nidulans; Fungo; Microbiologia; Poluente; Poluição ambiental. |
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Thesaurus Nal: |
Carcinogens. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02770naa a2200253 a 4500
001 1015437
005 2015-06-08
008 2005 bl uuuu u00u1 u #d
100 1 $aZUCCHI, T. D.
245 $aA short-term test adapted to detect the genotoxic effects of environmental volatile pollutants (benzene fumes) using the filamentous fungus Aspergillus nidulans.$h[electronic resource]
260 $c2005
520 $aWith the recent focus on environmental problems, increasing awareness of the harmful effects of industrial and agricultural pollution has created a demand for progressively more sophisticated pollutant and toxicity detection methods. Using Aspergillus nidulans strains this work presents a new short term-test that, most importantly, enables the rapid and inexpensive detection of volatile pollutants that induce genotoxic/carcinogenic effects in animals. The main aim is to contribute to environmental health protection, and special attention is directed to monitoring the hazard posed by benzene (as a carcinogenic agent model) mainly because its ubiquitous presence often leads to severe noxious effects in humans among whom increased rates of human leukemia have been reported. To evaluate even the submutagenic effects of benzene fumes, two Aspergillus nidulans diploid strains, heterozygous for several auxotrophic mutations, were used. The DNA lesions produced stimulate mitotic recombination and homozygotization of auxotrophic recessive mutations. Conidial exposure to a saturated atmosphere of benzene fumes for 20 s was enough to increase the mitotic recombination frequencies significantly. Genetic analyses of treated diploids evidenced alterations related to mitotic recombination frequencies, gene expression, and allelic segregation rates. Altogether they reflect the potential of benzene to induce alterations in the fungal DNA, and albeit indirectly, they also respond for the genotoxic/carcinogenic harmful side effects widely connected to benzene. This is the first description of a sensitive, rapid and inexpensive test able to detect the submutagenic dose effects of volatile environmental compounds. In addition, despite concentrating on benzene the same test can be applied to many other pollutants, volatile or not. Additionally, the test can also be used to detect the antigenotoxic properties of foods and drugs.
650 $aCarcinogens
650 $aAspergillus Nidulans
650 $aFungo
650 $aMicrobiologia
650 $aPoluente
650 $aPoluição ambiental
653 $aMicrobiologia ambiental
700 1 $aZUCCHI, F. D.
700 1 $aPOLI, P.
700 1 $aMELO, I. S. de
700 1 $aZUCCHI, T. M. A. D.
773 $tJournal of Environmental Monitoring, Washington, DC$gv. 7, n. 6, p. 598-602, 2005.
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Embrapa Meio Ambiente (CNPMA) |
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| Registros recuperados : 575 | |
| 7. |  | MELO, I. S. de. Bioremediation of pesticides in soils. In: INTERNATIONAL CONFERENCE, 2004, Buenos Aires, Argentina. Resumos...Monitoring systems and novel technologies for detection/removal of pollutants in/from ecosystems. Buenos Aires, Argentina, 2004. p.18-19. pág. 18-19| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 8. | | MELO, I. S. de. Biorremediação de áreas contaminadas. In: SEMINÁRIO SOBRE PERSPECTIVAS DA BIOTENOLOGIA EM PIRACICABA, 2001. Anais...Piracicaba: ESALQ- Escola Superior de Agricultura Luiz de Queiroz, p. 7-8, 2001| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| Registros recuperados : 575 | |
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