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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
18/06/2021 |
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Data da última atualização: |
18/06/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MELO, B. P.; LOURENCO, I. T.; FRAGA, O. T.; PINHEIRO, L. B.; LINS, C. B. de J.; MORGANTE, C. V.; ENGLER, J. A.; REIS, P. A. B.; SA, M. F. G. de; FONTES, E. P. B. |
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Afiliação: |
BRUNO PAES MELO, UFV; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; OTTO TEIXEIRA FRAGA, UFV; LUANNA BEZERRA PINHEIRO, UCB; CAMILA BARROZO DE JESUS LINS, INCT Plant Stress-Biotech; CAROLINA VIANNA MORGANTE, CPATSA; JANICE ALMEIDA ENGLER, INRAE, France; PEDRO AUGUSTO BRAGA REIS, UFV; MARIA FATIMA GROSSI DE SA, Cenargen; ELIZABETH PACHECO BATISTA FONTES, UFV. |
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Título: |
Contrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Scientific Reports, v. 11, 11178, 2021. |
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DOI: |
https://doi.org/10.1038/s41598-021-90767-6 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Isabela Tristan Lourenço-Tessutti; Maria Fátima Grossi-de-Sá. |
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Conteúdo: |
NACs are plant-specifc transcription factors involved in controlling plant development, stress responses, and senescence. As senescence-associated genes (SAGs), NACs integrate age- and stress-dependent pathways that converge to programmed cell death (PCD). In Arabidopsis, NACSAGs belong to well-characterized regulatory networks, poorly understood in soybean. Here, we interrogated the soybean genome and provided a comprehensive analysis of senescence-associated Glycine max (Gm) NACs. To functionally examine GmNAC-SAGs, we selected GmNAC065, a putative ortholog of Arabidopsis ANAC083/VNI2 SAG, and the cell death-promoting GmNAC085, an ANAC072 SAG putative ortholog, for analyses. Expression analysis of GmNAC065 and GmNAC085 in soybean demonstrated (i) these cell death-promoting GmNACs display contrasting expression changes during age- and stress-induced senescence; (ii) they are co-expressed with functionally diferent gene sets involved in stress and PCD, and (iii) are diferentially induced by PCD inducers. Furthermore, we demonstrated GmNAC065 expression delays senescence in Arabidopsis, a phenotype associated with enhanced oxidative performance under multiple stresses, higher chlorophyll, carotenoid and sugar contents, and lower stress-induced PCD compared to wild-type. In contrast, GmNAC085 accelerated stress-induced senescence, causing enhanced chlorophyll loss, ROS accumulation and cell death, decreased antioxidative system expression and activity. Accordingly, GmNAC065 and GmNAC085 targeted functionally contrasting sets of downstream AtSAGs, further indicating that GmNAC85 and
GmNAC065 regulators function inversely in developmental and environmental PCD. MenosNACs are plant-specifc transcription factors involved in controlling plant development, stress responses, and senescence. As senescence-associated genes (SAGs), NACs integrate age- and stress-dependent pathways that converge to programmed cell death (PCD). In Arabidopsis, NACSAGs belong to well-characterized regulatory networks, poorly understood in soybean. Here, we interrogated the soybean genome and provided a comprehensive analysis of senescence-associated Glycine max (Gm) NACs. To functionally examine GmNAC-SAGs, we selected GmNAC065, a putative ortholog of Arabidopsis ANAC083/VNI2 SAG, and the cell death-promoting GmNAC085, an ANAC072 SAG putative ortholog, for analyses. Expression analysis of GmNAC065 and GmNAC085 in soybean demonstrated (i) these cell death-promoting GmNACs display contrasting expression changes during age- and stress-induced senescence; (ii) they are co-expressed with functionally diferent gene sets involved in stress and PCD, and (iii) are diferentially induced by PCD inducers. Furthermore, we demonstrated GmNAC065 expression delays senescence in Arabidopsis, a phenotype associated with enhanced oxidative performance under multiple stresses, higher chlorophyll, carotenoid and sugar contents, and lower stress-induced PCD compared to wild-type. In contrast, GmNAC085 accelerated stress-induced senescence, causing enhanced chlorophyll loss, ROS accumulation and cell death, decreased antioxidative system expression and activity. Accordingly, GmNAC065 an... Mostrar Tudo |
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Palavras-Chave: |
GmNAC065 expression; NAC SAGs. |
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Thesagro: |
Genoma; Planta. |
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Thesaurus Nal: |
Arabidopsis; Soybeans. |
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Categoria do assunto: |
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G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/223872/1/s41598-021-90767-6.pdf
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Marc: |
LEADER 02705naa a2200325 a 4500
001 2132401
005 2021-06-18
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1038/s41598-021-90767-6$2DOI
100 1 $aMELO, B. P.
245 $aContrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses.$h[electronic resource]
260 $c2021
500 $aNa publicação: Isabela Tristan Lourenço-Tessutti; Maria Fátima Grossi-de-Sá.
520 $aNACs are plant-specifc transcription factors involved in controlling plant development, stress responses, and senescence. As senescence-associated genes (SAGs), NACs integrate age- and stress-dependent pathways that converge to programmed cell death (PCD). In Arabidopsis, NACSAGs belong to well-characterized regulatory networks, poorly understood in soybean. Here, we interrogated the soybean genome and provided a comprehensive analysis of senescence-associated Glycine max (Gm) NACs. To functionally examine GmNAC-SAGs, we selected GmNAC065, a putative ortholog of Arabidopsis ANAC083/VNI2 SAG, and the cell death-promoting GmNAC085, an ANAC072 SAG putative ortholog, for analyses. Expression analysis of GmNAC065 and GmNAC085 in soybean demonstrated (i) these cell death-promoting GmNACs display contrasting expression changes during age- and stress-induced senescence; (ii) they are co-expressed with functionally diferent gene sets involved in stress and PCD, and (iii) are diferentially induced by PCD inducers. Furthermore, we demonstrated GmNAC065 expression delays senescence in Arabidopsis, a phenotype associated with enhanced oxidative performance under multiple stresses, higher chlorophyll, carotenoid and sugar contents, and lower stress-induced PCD compared to wild-type. In contrast, GmNAC085 accelerated stress-induced senescence, causing enhanced chlorophyll loss, ROS accumulation and cell death, decreased antioxidative system expression and activity. Accordingly, GmNAC065 and GmNAC085 targeted functionally contrasting sets of downstream AtSAGs, further indicating that GmNAC85 and GmNAC065 regulators function inversely in developmental and environmental PCD.
650 $aArabidopsis
650 $aSoybeans
650 $aGenoma
650 $aPlanta
653 $aGmNAC065 expression
653 $aNAC SAGs
700 1 $aLOURENCO, I. T.
700 1 $aFRAGA, O. T.
700 1 $aPINHEIRO, L. B.
700 1 $aLINS, C. B. de J.
700 1 $aMORGANTE, C. V.
700 1 $aENGLER, J. A.
700 1 $aREIS, P. A. B.
700 1 $aSA, M. F. G. de
700 1 $aFONTES, E. P. B.
773 $tScientific Reports$gv. 11, 11178, 2021.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
31/05/2016 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
PINTO, M. G. F.; FAVARON, P. O.; ALCÂNTARA, D.; ANUNCIAÇÃO, A. R. A.; MIGLINO, M. A.; BORELLI, V.; FILHO, A. F. |
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Afiliação: |
MARIA G. F. PINTO, UFBA; PHELIPE O. FAVARON, FMVZ/USP; DAYANE ALCÂNTARA, FMVZ/USP; ADRIANA R. A. ANUNCIAÇÃO, FMVZ/USP; MARIA A. MIGLINO, FMVZ/USP; VICENTI BORELLI, FMVZ/USP; ANTONIO F. FILHO, FMVZ/USP. |
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Título: |
Patterns of arterial vascularization in swine hearts. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 5, p. 417-422, maio. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to characterize the patterns of arterial vascularization in swine hearts. Ninety swine hearts were submitted to the Spalteholz diaphanization technique in order to dissect the coronary arteries. Three types of arterial vascularization patterns were characterized through the behaviorof the rami circumflexus and interventricularis, namely: balanced, right and left types. The balanced pattern was the most frequently (42.2%); in this case, the rami circumflexus and interventricularis occupied their respective sulci. The right type (40%) was further categorized into three vascularization subtypes. In the first, ramus circumflexus dexter branched from the ramus interventricularis subsinuosus. In the second, the arteria coronaria dextra branched from ramus interventricularis subsinuosus and ramus circumflexus. In the third model, arteria coronaria sinister branched from ramus interventricularis paraconalis. The left type (17.7%) exhibited two subtypes. In the first, ramus interventricularis paraconalis ran through the entire corresponding sulcus and the ventral third of sulcus interventricularis subsinuosus, and ramus interventricularis subsinuosus occupied the dorsal and middle third of its respective sulcus. In the second, ramus interventricularis subsinuosus branched from arteria coronaria dextra and ran through the dorsal and medium thirds of its respective sulcus, and the ventral third was occupied by the collateral branch of ramus circumflexus sinister. Our results reinforce the thesis that the blood distribution system through the coronary artery in swine is similar to human, not only in qualitative but also by a quantitative comparison. MenosThis study aimed to characterize the patterns of arterial vascularization in swine hearts. Ninety swine hearts were submitted to the Spalteholz diaphanization technique in order to dissect the coronary arteries. Three types of arterial vascularization patterns were characterized through the behaviorof the rami circumflexus and interventricularis, namely: balanced, right and left types. The balanced pattern was the most frequently (42.2%); in this case, the rami circumflexus and interventricularis occupied their respective sulci. The right type (40%) was further categorized into three vascularization subtypes. In the first, ramus circumflexus dexter branched from the ramus interventricularis subsinuosus. In the second, the arteria coronaria dextra branched from ramus interventricularis subsinuosus and ramus circumflexus. In the third model, arteria coronaria sinister branched from ramus interventricularis paraconalis. The left type (17.7%) exhibited two subtypes. In the first, ramus interventricularis paraconalis ran through the entire corresponding sulcus and the ventral third of sulcus interventricularis subsinuosus, and ramus interventricularis subsinuosus occupied the dorsal and middle third of its respective sulcus. In the second, ramus interventricularis subsinuosus branched from arteria coronaria dextra and ran through the dorsal and medium thirds of its respective sulcus, and the ventral third was occupied by the collateral branch of ramus circumflexus sinister. Our r... Mostrar Tudo |
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Palavras-Chave: |
Circulação coronária; Coronary circulation; Modelo animal. |
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Thesagro: |
Aparelho circulatório; Coração. |
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Thesaurus NAL: |
Animal models; Cardiovascular system; Heart; Swine. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/143600/1/Patterns-of-arterial.pdf
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Marc: |
LEADER 02520naa a2200301 a 4500
001 2045977
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aPINTO, M. G. F.
245 $aPatterns of arterial vascularization in swine hearts.
260 $c2016
520 $aThis study aimed to characterize the patterns of arterial vascularization in swine hearts. Ninety swine hearts were submitted to the Spalteholz diaphanization technique in order to dissect the coronary arteries. Three types of arterial vascularization patterns were characterized through the behaviorof the rami circumflexus and interventricularis, namely: balanced, right and left types. The balanced pattern was the most frequently (42.2%); in this case, the rami circumflexus and interventricularis occupied their respective sulci. The right type (40%) was further categorized into three vascularization subtypes. In the first, ramus circumflexus dexter branched from the ramus interventricularis subsinuosus. In the second, the arteria coronaria dextra branched from ramus interventricularis subsinuosus and ramus circumflexus. In the third model, arteria coronaria sinister branched from ramus interventricularis paraconalis. The left type (17.7%) exhibited two subtypes. In the first, ramus interventricularis paraconalis ran through the entire corresponding sulcus and the ventral third of sulcus interventricularis subsinuosus, and ramus interventricularis subsinuosus occupied the dorsal and middle third of its respective sulcus. In the second, ramus interventricularis subsinuosus branched from arteria coronaria dextra and ran through the dorsal and medium thirds of its respective sulcus, and the ventral third was occupied by the collateral branch of ramus circumflexus sinister. Our results reinforce the thesis that the blood distribution system through the coronary artery in swine is similar to human, not only in qualitative but also by a quantitative comparison.
650 $aAnimal models
650 $aCardiovascular system
650 $aHeart
650 $aSwine
650 $aAparelho circulatório
650 $aCoração
653 $aCirculação coronária
653 $aCoronary circulation
653 $aModelo animal
700 1 $aFAVARON, P. O.
700 1 $aALCÂNTARA, D.
700 1 $aANUNCIAÇÃO, A. R. A.
700 1 $aMIGLINO, M. A.
700 1 $aBORELLI, V.
700 1 $aFILHO, A. F.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 5, p. 417-422, maio. 2016.
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