Data/hora: 08/06/2024 08:45:27

Biblioteca(s): | Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido.
Data corrente: | 29/05/2023
Data da última atualização: | 25/04/2024
Autoria: | PEREIRA, B. M.; ARRAES, F.; MARTINS, A. C. Q.; ALVES, N. S. F.; MELO, B. P.; MORGANTE, C. V.; SARAIVA, M. A. P.; SA, M. F. G. de; GUIMARAES, P. M.; BRASILEIRO, A. C. M.
Afiliação: | BRUNA MEDEIROS PEREIRA, UNIVERSIDADE DE BRASÍLIA; FABRÍCIO ARRAES, UNIVERSIDADE DE BRASILIA; ANDRESSA CUNHA QUINTANA MARTINS, UNIVERSIDADE CATOLICA DE BRASILIA; NAYARA SABRINA FREITAS ALVES, UNIVERSIDADE FEDERAL DO PARANÁ; BRUNO PAES MELO, LONGPING HIGH TECH; CAROLINA VIANNA MORGANTE, CPATSA; MARIO ALFREDO PASSOS SARAIVA, INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIA; MARIA FATIMA GROSSI DE SA, CENARGEN; PATRICIA MESSEMBERG GUIMARAES, CENARGEN; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN.
Título: | A novel soybean hairy root system for gene functional validation.
Ano de publicação: | 2023
Fonte/Imprenta: | Plos One, v. 18, n. 5, e0285504, 2023.
DOI: | https://doi. org/10.1371/journal.pone.0285504
Idioma: | Inglês
Notas: | Na publicação: Maria Fátima Grossi-de-Sa; Patricia Messenberg Guimaraes.
Conteúdo: | Agrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suitable for high throughput in root analysis of candidate genes in soybean.
Palavras-Chave: | Agrobacterium rhizogenes; Transgenic soybean.
Thesagro: | Glycine Max; Melhoramento Genético Vegetal; Meloidogyne spp; Soja.
Thesaurus Nal: | Soybeans.

Categoria do assunto:

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G Melhoramento Genético

URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1154066/1/A-novel-soybean-hairy-root-system-for-gene-functional-validation-2023.pdf
Registro original: | Embrapa Semiárido (CPATSA)

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CENARGEN - (39167-1) - UPC - AP - DD -  -  -  - DIS
CPATSA - (60434-1) - UPC - AP - DD -  -  -  - DIS