Data/hora: 18/06/2024 15:18:29

Biblioteca(s): | Embrapa Agroindústria de Alimentos; Embrapa Arroz e Feijão.
Data corrente: | 25/06/2012
Data da última atualização: | 22/02/2016
Autoria: | DINON, A. Z.; BROD, F. C. A.; MELLO, C. S.; OLIVEIRA, E. M. M.; FARIA, J. C.; ARISI, A. C. M.
Afiliação: | ANDRÉIA Z. DINON, UFSC; FÁBIO C. A. BROD, UFSC; CARLA S. MELLO, UFSC; EDNA MARIA MORAIS OLIVEIRA, CTAA; JOSIAS CORREA DE FARIA, CNPAF; ANA C. M. ARISI, UFSC.
Título: | Primers and probes development for specific PCR detection of genetically modified common bean (Phaseolus vulgaris) Embrapa 5.1.
Ano de publicação: | 2012
Fonte/Imprenta: | Journal of Agricultural and Food Chemistry, Easton, v. 60, n. 18, p. 4672-4677, May 2012.
DOI: | 10.1021/jf3011257
Idioma: | Inglês
Conteúdo: | The genetically modified common bean Embrapa 5.1, developed by Brazilian Agricultural Research Corporation (Embrapa), is the first commercial GM plant produced in Latin America. It presents high resistance to the Bean golden mosaic virus. In this work, primers and probes targeting a taxon-specific reference DNA sequence for the common bean (Phaseolus vulgaris L.) and a construct-specific DNA sequence of Embrapa 5.1 GM common bean were successfully developed. The primers and probes showed high specificity for the target detection. Both methods showed suitable efficiency and performance to be used as an endogenous target for detection of common bean DNA and for construct-specific detection of GM common bean Embrapa 5.1, respectively. Both real-time PCR assays proved to be valuable for future assessment of interlaboratory studies.
Palavras-Chave: | Organismo geneticamente modificado; PCR.
Thesagro: | Biotecnologia; Engenharia genética; Feijão; Phaseolus vulgaris.
Thesaurus Nal: | Beans; Genetically modified organisms; Polymerase chain reaction; Transgenic plants.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
Registro original: | Embrapa Agroindústria de Alimentos (CTAA)

Exemplares:

CNPAF - (31429-1) - UPC - AP - DD - 2012.016 -  - 2012.016 - DIS
CTAA - (11655-1) - UPC - AP - PP - 2012/210 -  - 2012.00210 - DIS