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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
22/03/2016 |
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Data da última atualização: |
19/06/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PÉREZ-MONTAÑO, F.; CERRO, P. del; JIMÉNEZ-GUERRERO, I.; LÓPEZ-BAENA, F. J.; CUBO, M. T.; HUNGRIA, M.; MEGÍAS, M.; OLLERO, F. J. |
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Afiliação: |
Francisco Péres-Montaño, Universidade de Sevilla; Pablo del Cerro, Universidade de Sevilia; Irene Jiménez-Guerreiro, Universidade de Sevilla; Francisco Javier López-Baena, Universidade de Sevilla; maria Teresa Cubo, Universidade de Sevilla; MARIANGELA HUNGRIA DA CUNHA, CNPSO; Manuel Megías, Universidade de Sevilla; Francisco Javier Ollero, Uiversidade de Sevilla. |
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Título: |
RNA-seq analysis of the Rhizobium tropici CIAT 899 Transcripitome shows similarites in the activation patterns of symbiotic genes in the presence of apigenin and salt. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
BMC Genomics, v. 17, n. 1, p. 198, Mar. 2016. |
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ISSN: |
1471-2164 |
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DOI: |
10.1186/s12864-016-2543-3 |
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Idioma: |
Inglês |
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Conteúdo: |
Rhizobium tropici strain CIAT 899 establishes effective symbioses with several legume species, including Phaseolus vulgaris and Leucaena leucocephala. This bacterium synthesizes a large variety of nodulation factors in response to nod-gene inducing flavonoids and, surprisingly, also under salt stress conditions. The aim of this study was to identify differentially expressed genes in the presence of both inducer molecules, and analyze the promoter regions located upstream of these genes. Results obtained by RNA-seq analyses of CIAT 899 induced with apigenin, a nod gene-inducing flavonoid for this strain, or salt allowed the identification of 19 and 790 differentially expressed genes, respectively. Fifteen of these genes were up-regulated in both conditions and were involved in the synthesis of both Nod factors and indole-3-acetic acid. Transcription of these genes was presumably activated through binding of at least one of the five NodD proteins present in this strain to specific nod box promoter sequences when the bacterium was induced by both apigenin and salt. Finally, under saline conditions, many other transcriptional responses were detected, including an increase in the transcription of genes involved in trehalose catabolism, chemotaxis and protein secretion, as well as ribosomal genes, and a decrease in the transcription of genes involved in transmembrane transport. To our knowledge this is the first time that a transcriptomic study shows that salt stress induces the expression of nodulation genes in the absence of flavonoids. Thus, in the presence of both nodulation inducer molecules, apigenin and salt, R. tropici CIAT 899 up-regulated the same set of symbiotic genes. It could be possible that the increases in the transcription levels of several genes related to nodulation under saline conditions could represent a strategy to establish symbiosis under abiotic stressing conditions. MenosRhizobium tropici strain CIAT 899 establishes effective symbioses with several legume species, including Phaseolus vulgaris and Leucaena leucocephala. This bacterium synthesizes a large variety of nodulation factors in response to nod-gene inducing flavonoids and, surprisingly, also under salt stress conditions. The aim of this study was to identify differentially expressed genes in the presence of both inducer molecules, and analyze the promoter regions located upstream of these genes. Results obtained by RNA-seq analyses of CIAT 899 induced with apigenin, a nod gene-inducing flavonoid for this strain, or salt allowed the identification of 19 and 790 differentially expressed genes, respectively. Fifteen of these genes were up-regulated in both conditions and were involved in the synthesis of both Nod factors and indole-3-acetic acid. Transcription of these genes was presumably activated through binding of at least one of the five NodD proteins present in this strain to specific nod box promoter sequences when the bacterium was induced by both apigenin and salt. Finally, under saline conditions, many other transcriptional responses were detected, including an increase in the transcription of genes involved in trehalose catabolism, chemotaxis and protein secretion, as well as ribosomal genes, and a decrease in the transcription of genes involved in transmembrane transport. To our knowledge this is the first time that a transcriptomic study shows that salt stress induces the e... Mostrar Tudo |
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Palavras-Chave: |
Lipochitooligosaccharides; Nod factors; Nodutlation; RNA-seq. |
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Thesagro: |
Nodulação; Rhizobium. |
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Thesaurus Nal: |
Apigenin; Rhizobium tropici; Salt stress. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142584/1/RNA-seq-analysis-of-the-rhizobium...pdf
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Marc: |
LEADER 02939naa a2200337 a 4500
001 2041600
005 2017-06-19
008 2016 bl uuuu u00u1 u #d
022 $a1471-2164
024 7 $a10.1186/s12864-016-2543-3$2DOI
100 1 $aPÉREZ-MONTAÑO, F.
245 $aRNA-seq analysis of the Rhizobium tropici CIAT 899 Transcripitome shows similarites in the activation patterns of symbiotic genes in the presence of apigenin and salt.$h[electronic resource]
260 $c2016
520 $aRhizobium tropici strain CIAT 899 establishes effective symbioses with several legume species, including Phaseolus vulgaris and Leucaena leucocephala. This bacterium synthesizes a large variety of nodulation factors in response to nod-gene inducing flavonoids and, surprisingly, also under salt stress conditions. The aim of this study was to identify differentially expressed genes in the presence of both inducer molecules, and analyze the promoter regions located upstream of these genes. Results obtained by RNA-seq analyses of CIAT 899 induced with apigenin, a nod gene-inducing flavonoid for this strain, or salt allowed the identification of 19 and 790 differentially expressed genes, respectively. Fifteen of these genes were up-regulated in both conditions and were involved in the synthesis of both Nod factors and indole-3-acetic acid. Transcription of these genes was presumably activated through binding of at least one of the five NodD proteins present in this strain to specific nod box promoter sequences when the bacterium was induced by both apigenin and salt. Finally, under saline conditions, many other transcriptional responses were detected, including an increase in the transcription of genes involved in trehalose catabolism, chemotaxis and protein secretion, as well as ribosomal genes, and a decrease in the transcription of genes involved in transmembrane transport. To our knowledge this is the first time that a transcriptomic study shows that salt stress induces the expression of nodulation genes in the absence of flavonoids. Thus, in the presence of both nodulation inducer molecules, apigenin and salt, R. tropici CIAT 899 up-regulated the same set of symbiotic genes. It could be possible that the increases in the transcription levels of several genes related to nodulation under saline conditions could represent a strategy to establish symbiosis under abiotic stressing conditions.
650 $aApigenin
650 $aRhizobium tropici
650 $aSalt stress
650 $aNodulação
650 $aRhizobium
653 $aLipochitooligosaccharides
653 $aNod factors
653 $aNodutlation
653 $aRNA-seq
700 1 $aCERRO, P. del
700 1 $aJIMÉNEZ-GUERRERO, I.
700 1 $aLÓPEZ-BAENA, F. J.
700 1 $aCUBO, M. T.
700 1 $aHUNGRIA, M.
700 1 $aMEGÍAS, M.
700 1 $aOLLERO, F. J.
773 $tBMC Genomics$gv. 17, n. 1, p. 198, Mar. 2016.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 55 | |
| 41. |  | MATEOS, C.; GÓMEZ, R. B.; GARCIA-TRIGUEROS, C.; CHUEIRE, L. M. O.; LAMRABET, Y.; HUNGRIA, M.; MANYANI, H.; MEGÍAS, M.; SOUSA, C. Genotypic and phenotypic characterization of soybean sinorhizobial strains isolated from brazilian soils. In: INTERNATIONAL CONGRESS ON NITROGEN FIXATION, 12., 1999, Foz do Iguacu. Nitrogen fixation: from molecules to crop productivity: proceedings. Dordrechet: Kluwer, 2000. p. 191. (Current Plant Science and Biotechnology in Agriculture, 38). Editado por Fabio O. Pedrosa, Mariangela Hungria, Geoffrey Yates, William E. Newton.| Biblioteca(s): Embrapa Soja. |
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| 42. | | HUNGRIA, M.; CHUEIRE, L. M. O.; ANDRADE, D. S.; PROBANZA, A.; GUTTIERREZ-MANERO, F.J.; MOSTASSO, L.; VARGAS, M. A. T.; MEGIAS, M. Selection of Rhizobium strains for the common bean crop. In: INTERNATIONAL CONGRESS ON NITROGEN FIXATION, 12., 1999, Foz do Iguacu. Nitrogen fixation: from molecules to crop productivity - proceedings. Dordrechet: Kluwer, 2000. p.624. (Current Plant Science and Biotechnology in Agriculture, v.38). Editado por Fabio O. Pedrosa, Mariangela Hungria, Geoffrey Yates, William E. Newton.| Biblioteca(s): Embrapa Cerrados. |
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| 43. | | HUNGRIA, M.; CHUEIRE, L. M. de O.; ANDRADE, D. S.; PROBANZA, A.; GUTTIERREZ-MAÑERO, F. J.; MOSTASSO, L.; VARGAS, M. A. T.; MEGIAS, M. Selection of Rhizobium strains for the common bean crop. In: INTERNATIONAL CONGRESS ON NITROGEN FIXATION, 12., 1999, Foz do Iguacu. Nitrogen fixation: from molecules to crop productivity: proceedings. Dordrechet: Kluwer, 2000. p. 624. (Current Plant Science and Biotechnology in Agriculture, 38). Editado por Fabio O. Pedrosa, Mariangela Hungria, Geoffrey Yates, William E. Newton.| Biblioteca(s): Embrapa Soja. |
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| 44. | | GOMES, D. F; TULLIO, L. D.; DEL CERRO, P.; NAKATANI, A. S.; ROLLA-SANTOS, A. A. P.; GIL-SERRANO, A.; MEGÍAS, M.; OLLERO, F. J.; HUNGRIA, M. Regulation of hsnT, nodF and nodE genes in Rhizobium tropici CIAT 899 and their roles in the synthesis of Nod factors and in the symbiosis. Microbiology, v. 165, p. 990-1000, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja. |
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| 46. | | ORMEÑO-ORRILLO, E.; GOMES, D. F.; CERRO, P. del; VASCONCELOS, A. T. R.; CANCHAYA, C.; ALMEIDA, L. G. P.; MERCANTE, F. M.; JAVIER OLLERO, F.; MEGÍAS, M.; HUNGRIA, M. Genome of Rhizobium leucaenae strains CFN 299T and CPAO 29.8: searching for genes related to a successful symbiotic performance under stressful conditions. BMC Genomics, v. 17, n. 534, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agropecuária Oeste; Embrapa Soja. |
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| 47. | | CERRO, P. del; ROLLA-SANTOS, A. A. P.; GOMES, D. F.; MARKS, B. B.; PÉREZ-MONTAÑO, F.; RODRÍGUEZ-CARVAJAL, M. A.; NAKATANI, A. S.; GIL-SERRANO, A.; MEGÍAS, M.; OLLERO, F. J.; HUNGRIA, M. Regulatory nodD1 and nodD2 genes of Rhizobium tropici strain CIAT 899 and their roles in the early stages of molecular signaling and host-legume nodulation. BMC Genomics, London, [S. l.], v. 16, n. 251, Mar. 2015. 13 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 48. | | FAGOTTI, D. dos S. L.; ABRANTES, J. L. F.; CEREZINI, P.; FUKAMI, J.; NOGUEIRA, M. A.; CERRO, P. del; VALDERRAMA-FERNANDEZ, R.; OLLERO, F. J.; MEGIAS, M.; HUNGRIA, M. Quorum sensing communication: Bradyrhizobium-Azospirillum interaction via N-acyl-homoserine lactones in the promotion of soybean symbiosis. Journal of Basic Microbiology, v. 59, p. 38-53, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 49. | | SANTOS, I. R.; RIBEIRO, D. G.; TÁVORA, F. T. P. K.; MAXIMIANO, M. R.; RABELO, A. C.; RIOS, T. B.; REIS JUNIOR, F. B. dos; MEGÍAS, M.; SILVA, L. P. da; REIS, A. M. dos. Priming of defense-related genes in Brassica oleracea var. capitata using concentrated metabolites produced by Rhizobium tropici CIAT 899. Brazilian Journal of Microbiology, 2022. On-line first. Na publicação: Fábio Bueno Reis Junior, Luciano Paulino Silva, Angela Mehta.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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| 50. | | CERRO, P. del; ROLLA-SANTOS, A. A. P.; VALDERRAMA-FERNANDEZ, R.; GIL-SERRANO, A.; BELLOGÍN, R. A.; GOMES, D. F.; MONTAÑO, F. P.; MEGÍAS, M.; HUNGRIA, M.; JAVIER OLLERO, F. NrcR, a new transcriptional regulator of Rhizobium tropici CIAT 899 involved in the Legume root-nodule symbiosis. PLOS One, v. 11, n. 4, e0154029, Apr. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 51. | | PINTO, F. G. S.; CHUEIRE, L. M. O.; VASCONCELOS, A. T. R.; NICOLÁS, M. F.; ALMEIDA, L. G. P.; SOUZA, R. C.; MENNA, P.; BARCELLOS, F. G.; MEGÍAS, M.; HUNGRIA, M. Novel genes related to nodulation, secretion systems, and surface structures revealed by a genome draft of Rhizobium tropici strain PRF 81. Functional & Integrative Genomics, Heidelberg, v. 9, n. 2, p. 263-270, May 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 52. | | CERRO, P. del; AYALA-GARCÍA, P.; JIMÉNEZ-GUERRERO, I.; LÓPEZ-BAENA, F. J.; VINARDELL, J. M.; MEGÍAS, M.; HUNGRIA, M.; GIL-SERRANO, A. M.; PÉREZ-MONTÃNO, F.; OLLERO, F. J. The non-flavonoid inducible nodA3 and the flavonoid regulated nodA1 genes of Rhizobium tropici CIAT 899 guarantee nod factor production and nodulation of different host legumes. Plant and Soil, v. 440, p. 185-200, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 53. | | CERRO, P. del; ROLLA-SANTOS, A. A. P.; GOMES, D. F.; MARKS, B. B.; ESPUNY, M. del R.; RODRÍGUEZ-CARVAJAL, M. A.; SORIA-DÍAZ, M. E.; NAKATANI, A. S.; HUNGRIA, M.; JAVIER OLLERO, F.; MEGÍAS, M. Opening the "black box" of nodD3, nodD4 and nodD5 genes of Rhizobium tropici strain CIAT 899. BMC Genomics, v. 16, n. 1, p. 864, Oct. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 54. | | RODRIGUEZ-NAVARRO, D. N.; BELLOGIN, R.; BUENDIA, A. M.; CAMACHO, M.; CUBO, T.; CHUEIRE, L. M. O.; DAZA, A.; LYRA, M. C.; ESPUNY, R.; GOMEZ, R.; HUNGRIA, M.; MANYANI, H.; MEGÍAS, M.; OLLERO, F. J.; RUIZ-SAINZ, J. E.; SANTAMARIA, C.; SOUSA, C.; TEMPRANO, F.; YANG, S. S.; ZHOU, J. C. Biodiversity and selection of Sinorhizobium fredii strains for soybean inoculants. In: INTERNATIONAL CONGRESS ON NITROGEN FIXATION, 12., 1999, Foz do Iguacu. Nitrogen fixation: from molecules to crop productivity: proceedings. Dordrechet: Kluwer, 2000. p. 183-184. (Current Plant Science and Biotechnology in Agriculture, 38). Editado por Fabio O. Pedrosa, Mariangela Hungria, Geoffrey Yates, William E. Newton.| Biblioteca(s): Embrapa Soja. |
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| 55. | | ORMEÑO-ORRILLO, E.; MENNA, P.; ALMEIDA, L. G. P.; JAVIER OLLERO, F.; NICOLÁS, M. F.; RODRIGUES, E. P.; NAKATANI, A. S.; BATISTA, J. S. S.; CHUEIRE, L. M. de O.; SOUZA, R. C.; VASCONCELOS, A. T. R.; MEGÍAS, M.; HUNGRIA, M.; MARTÍNEZ-ROMERO, E. Genomic basis of broad host range and environmental adaptability of Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 which are used in inoculants for common bean (Phaseolus vulgaris L.). BMC Genomics, v. 13, n. 735, 2012. 26 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 55 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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