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| Registros recuperados : 31 | |
| 23. |  | FERREIRA, P. da S.; MEDEIROS, N.; MELO, L. E. de L.; SOUZA, F. I. B. de; MORI, F. A.; GOULART, S. L. Propriedades da madeira de Sterculia apetala e sua qualidade para nidificação da arara-zul na Amazônia. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DA MADEIRA, 3., 2017, Florianópolis. Trabalhos publicados. [Seropédica]: SBCTEM, 2017.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 24. | | ROSA, M. S.; FANCELLI, M.; MEDEIROS, N. G. F. de; RAGNI, M.; KALILE, M. O.; MAGALHÃES, D. G. de; LEDO, C. A. da S.; FERREIRA, C. F. Resposta de Diaphorina citri Kuwayama (Hemiptera: Liviidae) a ondas mecânicas. In : JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 12., 2018. Ciência profissional : resumos. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2019. 1 p.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 25. | | BARROS, B. G. de F.; FREITAS, A. D. S. de; SOUZA, R. J. C. de; FERREIRA NETO, R. A.; SILVA, A. A. da; MEDEIROS, N. V. da S. Teores de fósforo em biofertilizante com ordem de fungos mucolares. In: SIMPÓSIO DE MUDANÇAS CLIMÁTICAS E DESERTIFICAÇÃO NO SEMIÁRIDO BRASILEIRO, 4., 2015, Petrolina. Experiências e oportunidades para o desenvolvimento. Petrolina: Embrapa Semiárido, 2015. 1 CD-ROM. (Embrapa Semiárido. Documentos, 262).| Biblioteca(s): Embrapa Semiárido. |
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| 26. | | SILVA, A. A. da; SOUZA, R. J. C. de; FERREIRA NETO, R. A.; BARROS, B. G. de F.; BERGER, L. R. R.; FREITAS, A. D. S. de; MEDEIROS, N. V. Biofertilizantes como alternativa à utilização de fertilizantes nitrogenados: uma opção para o Semiárido. In: SIMPÓSIO DE MUDANÇAS CLIMÁTICAS E DESERTIFICAÇÃO NO SEMIÁRIDO BRASILEIRO, 4., 2015, Petrolina. Experiências e oportunidades para o desenvolvimento. Petrolina: Embrapa Semiárido, 2015. 1 CD-ROM. (Embrapa Semiárido. Documentos, 262).| Biblioteca(s): Embrapa Semiárido. |
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| 27. | | SANTOS, M. G. O. dos; LIMA, S. C. P. de; CARVALHO, M. das G. X. de; MEDEIROS, N. G. de A.; SILVA, L. M. de; XAVIER, V. M. C. Monitoramento da eficiência de pasteurização de leite de cabra, baseado no sistema de análise de perigos e pontos críticos de controle, em mini-usinas do cariri paraibano. Higiene Alimentar, São Paulo, v. 20, n. 141, p. 106-110, maio/jun. 2006.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 28. | | SANTOS, M. G. O. dos; LIMA, S. C. P. de; CARVALHO, M. das G. X. de; MEDEIROS, N. G. de A.; SILVA, L. M. de; XAVIER, V. M. C. Monitoramento da eficiência de pasteurização de leite de cabra, baseado no sistema de análise de perigos e pontos críticos de controle, em mini-usinas do cariri paraibano. Higiene Alimentar, São Paulo, v. 20, n. 141, p. 106-110, maio/jun., 2006.| Biblioteca(s): Embrapa Amapá. |
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| 29. | | ROSA, M. S.; FANCELLI, M.; MEDEIROS, N. G. F. de; RAMOS, C. E. C. de O.; SILVA, J. N. da; RGÔLO, R. da S.; FERREIRA, C. F.; LEDO, C. A. da S. Efeito do protótipo emissor de ondas mecânicas sobre o comportamento de Diaphorina citri Kuwayama (Hemiptera: Liviidae) In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 17., 2017 Ciência e Empreendedorismo : resumos. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2017. 137p. 1p. Manejo das principais doenças e insetos-praga| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 30. | | SOUSA, F. F. DE; MEDEIROS, N. I.; ARRIEL, N. H. C.; LUCENA, A. M. A. DE; VASCONCELOS, G. C. L.; MEDEIROS, K. A. A. DE; MEDEIROS, O. DE S.; FREIRE, M. A. DE O. Caracterização de genótipos de pinhão manso do banco ativo de germoplasma da embrapa algodão. In: CONGRESSO BRASILEIRO DE PESQUISA EM PINHÃO-MANSO, 2., 2011, Brasília, DF. Pinhão-manso: focando em soluções sustentáveis para produção de biocombustíveis: anais. Brasília, DF: Embrapa Agroenergia: ABPPM, 2011. 2 p. 1 CD-ROM. (Embrapa Agroenergia. Documentos, 005).| Biblioteca(s): Embrapa Algodão. |
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| 31. | | LIMA, S. C. P. de; SANTOS, M. G. O. dos; CARVALHO, M. das G. X. de; SILVA, L. M.; MEDEIROS, N. G. de A.; XAVIER, V. M. S. C.; HOLANDA, S. A. de M. Características microbiológicas do leite de cabra cru e pasteurizado em seis mini-usinas do cariri paraibano. Higiene Alimentar, São Paulo, v. 20, n. 142, p. 79-84, jul. 2006.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 31 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
29/12/2014 |
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Data da última atualização: |
07/04/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
BENCKE-MALATO, M.; CABREIRA, C.; WIEBKE-STROHM, B.; BÜCKER-NETO, L.; MANCINI, E.; OSORIO, M. B.; HOMRICH, M. S.; TURCHETTO-ZOLET, A. C.; CARVALHO, M. C. C. G. de; STOLF, R.; WEBER, R. L. M.; WESTERGAARD, G.; CASTAGNARO, A. P.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C.; MARGIS-PINHEIRO, M.; BODANESE-ZANETTINI, M. H. |
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Afiliação: |
MARTA BENCKE-MALATO, UFRGS; CAROLINE CABREIRA, UFRGS; BEATRIZ WIEBKE-STROHM, UFRGS; LAURO BÜCKER-NETO, UFRGS; ESTEFANIA MANCINI, Instituto de Agrobiotecnologia Rosario SA; MARINA B. OSORIO, UFRGS; MILENA S. HOMRICH, UFRGS; ANDREIA CARINA TURCHETTO-ZOLET, UFRGS; MAYRA C. C. G. DE CARVALHO, CNPSO - estagiária; RENATA STOLF, CNPSO - estagiària; RICARDO L. M. WEBER, UFRGS; GASTÓN WESTERGAARD, Instituto de Agrobiotecnologia Rosario SA; ATÍLIO P. CASTAGNARO, Estación Experimental Agroindustrial Obispo Colombres (EEAOC); RICARDO VILELA ABDELNOOR, CNPSO; FRANCISMAR CORREA MARCELINO-GUIMARÃES, CNPSO; MÁRCIA MARGIS-PINHEIRO, UFRGS; MARIA HELENA BODANESE-ZANETTINI, UFRGS. |
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Título: |
Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
BMC Plant Biology, v. 14, n. 1, article 236, Sept. 2014. |
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Páginas: |
18 p. |
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ISSN: |
1471-2229 |
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DOI: |
10.1186/s12870-014-0236-0 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified. Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants. Conclusions: The present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants. MenosBackground: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified. Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than ... Mostrar Tudo |
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Thesagro: |
Soja. |
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Thesaurus NAL: |
Soybeans. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/114583/1/Genome-wide-annotation-of-the-soybean-WRKY-family-and-functional-characterization-of-genes-involved-in-response-to-Phakopsora-pachyrhizi-infection.pdf
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Marc: |
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022 $a1471-2229
024 7 $a10.1186/s12870-014-0236-0$2DOI
100 1 $aBENCKE-MALATO, M.
245 $aGenome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection.$h[electronic resource]
260 $c2014
300 $a18 p.
520 $aBackground: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified. Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants. Conclusions: The present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants.
650 $aSoybeans
650 $aSoja
700 1 $aCABREIRA, C.
700 1 $aWIEBKE-STROHM, B.
700 1 $aBÜCKER-NETO, L.
700 1 $aMANCINI, E.
700 1 $aOSORIO, M. B.
700 1 $aHOMRICH, M. S.
700 1 $aTURCHETTO-ZOLET, A. C.
700 1 $aCARVALHO, M. C. C. G. de
700 1 $aSTOLF, R.
700 1 $aWEBER, R. L. M.
700 1 $aWESTERGAARD, G.
700 1 $aCASTAGNARO, A. P.
700 1 $aABDELNOOR, R. V.
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aMARGIS-PINHEIRO, M.
700 1 $aBODANESE-ZANETTINI, M. H.
773 $tBMC Plant Biology$gv. 14, n. 1, article 236, Sept. 2014.
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Embrapa Soja (CNPSO) |
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