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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
26/07/2018 |
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Data da última atualização: |
26/07/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARVALHO, A. L. de A.; REZENDE, L. C. de; COSTA, L. B.; HALFELD-VIEIRA, B. de A.; PINTO, Z. V.; MORANDI, M. A. B.; MEDEIROS, F. H. V.; BETTIOL, W. |
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Afiliação: |
ANDRE LUIZ DE ANDRADE CARVALHO, UFV; LARISSA CASTRO DE REZENDE, UFV; LUCIO BERTOLDO COSTA, FCA-UNESP; BERNARDO DE ALMEIDA HALFELD VIEIRA, CNPMA; ZAYAME VEGETTE PINTO, FCA-UNESP; MARCELO AUGUSTO BOECHAT MORANDI, CNPMA; FLAVIO HENRIQUE VASCONCELOS DE MEDEIROS, UFV; WAGNER BETTIOL, CNPMA. |
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Título: |
Optimizing the mass production of Clonostachys rosea by liquid-state fermentation. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Biological Control, v. 118, p. 16-25, 2018. |
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DOI: |
https://doi.org/10.1016/j.biocontrol.2017.11.014 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Optimization of the culture medium and the entire mass production process for various fungi in liquid medium has been studied. However, the technology is not well developed for Clonostachys rosea, a biocontrol agent against various plant pathogens due to its antagonistic capacity to act as a hyperparasite, compete for nutrients and space, and induce plant resistance to pathogens. In this study, we aimed to optimize the culture medium and to standardize parameters that may interfere with the production of C. rosea conidia in a liquid-state fermentation system. Culturing was performed in 250-mL Erlenmeyer flasks shaken for 7 days, followed by planned experimental methodology to reduce the number of analyses and consumable costs. Benchtop bioreactor tests with the optimized medium were performed. Glucose and sucrose were evaluated as carbon sources. Initially, the effects of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on inoculum production were evaluated, with the pH and photoperiod being factors that contributed to conidial production. Optimization of the fermentation conditions was performed using a central composite rotational design (CCD) with a wider range of pH values and photoperiods. The remaining variables were fixed according to the previous assay. Colony-forming unit (CFUs), biomass production and conidial viability were evaluated, and glucose was used as a carbon source to enhance conidial production. The optimized conditions that resulted in a maximum yield of conidia (1.78 x 107 conidia?mL-1), dried biomass (0.558?g) and CFUs (5.15 x 106 CFUs?mL-1) were a pH value of 4 and a photoperiod of 12?h. MenosAbstract: Optimization of the culture medium and the entire mass production process for various fungi in liquid medium has been studied. However, the technology is not well developed for Clonostachys rosea, a biocontrol agent against various plant pathogens due to its antagonistic capacity to act as a hyperparasite, compete for nutrients and space, and induce plant resistance to pathogens. In this study, we aimed to optimize the culture medium and to standardize parameters that may interfere with the production of C. rosea conidia in a liquid-state fermentation system. Culturing was performed in 250-mL Erlenmeyer flasks shaken for 7 days, followed by planned experimental methodology to reduce the number of analyses and consumable costs. Benchtop bioreactor tests with the optimized medium were performed. Glucose and sucrose were evaluated as carbon sources. Initially, the effects of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on inoculum production were evaluated, with the pH and photoperiod being factors that contributed to conidial production. Optimization of the fermentation conditions was performed using a central composite rotational design (CCD) with a wider range of pH values and photoperiods. The remaining variables were fixed according to the previous assay. Colony-forming unit (CFUs), biomass production and conidial viability were evaluated, and glucose was used as a carbon source to enhance conidial production. The optimized conditions th... Mostrar Tudo |
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Palavras-Chave: |
Bioreactor; Liquid media; Produção massal. |
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Thesagro: |
Fungo Para Controle Biológico. |
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Thesaurus Nal: |
Biological control agents; Biomass production; Bioreactors; Clonostachys rosea. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
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008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.biocontrol.2017.11.014$2DOI
100 1 $aCARVALHO, A. L. de A.
245 $aOptimizing the mass production of Clonostachys rosea by liquid-state fermentation.$h[electronic resource]
260 $c2018
520 $aAbstract: Optimization of the culture medium and the entire mass production process for various fungi in liquid medium has been studied. However, the technology is not well developed for Clonostachys rosea, a biocontrol agent against various plant pathogens due to its antagonistic capacity to act as a hyperparasite, compete for nutrients and space, and induce plant resistance to pathogens. In this study, we aimed to optimize the culture medium and to standardize parameters that may interfere with the production of C. rosea conidia in a liquid-state fermentation system. Culturing was performed in 250-mL Erlenmeyer flasks shaken for 7 days, followed by planned experimental methodology to reduce the number of analyses and consumable costs. Benchtop bioreactor tests with the optimized medium were performed. Glucose and sucrose were evaluated as carbon sources. Initially, the effects of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on inoculum production were evaluated, with the pH and photoperiod being factors that contributed to conidial production. Optimization of the fermentation conditions was performed using a central composite rotational design (CCD) with a wider range of pH values and photoperiods. The remaining variables were fixed according to the previous assay. Colony-forming unit (CFUs), biomass production and conidial viability were evaluated, and glucose was used as a carbon source to enhance conidial production. The optimized conditions that resulted in a maximum yield of conidia (1.78 x 107 conidia?mL-1), dried biomass (0.558?g) and CFUs (5.15 x 106 CFUs?mL-1) were a pH value of 4 and a photoperiod of 12?h.
650 $aBiological control agents
650 $aBiomass production
650 $aBioreactors
650 $aClonostachys rosea
650 $aFungo Para Controle Biológico
653 $aBioreactor
653 $aLiquid media
653 $aProdução massal
700 1 $aREZENDE, L. C. de
700 1 $aCOSTA, L. B.
700 1 $aHALFELD-VIEIRA, B. de A.
700 1 $aPINTO, Z. V.
700 1 $aMORANDI, M. A. B.
700 1 $aMEDEIROS, F. H. V.
700 1 $aBETTIOL, W.
773 $tBiological Control$gv. 118, p. 16-25, 2018.
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Embrapa Meio Ambiente (CNPMA) |
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| Registros recuperados : 27 | |
| 4. |  | MEDEIROS, F. H. V.; BETTIOL, W.; SOUZA, R. M. de. Efeito do tratamento preventivo e curativo com leite e teste de suas fontes e frações no controle do oídio da abobrinha. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 39., 2006, Salvador, BA. Resumos... Salvador, BA: SBF, 2006. Revista Fitopatologia Brasileira, Brasília, DF, v.31, supl., p. S.207, ago. 2006.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 7. | | FARIA, A. F. de; SCHULMAN, P.; MEYER, M. C.; CRUZ-MAGALHÃES, V.; MEDEIROS, F. H. V. Sete anos de eficácia de produtos de biocontrole de mofo branco na germinação carpogênica de Sclerotinia sclerotiorum no Brasil: uma metanálise. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 53., 2023, Brasília, DF. Anais... Brasília, DF: SBF, 2023. resumo. p. 221. Título em inglês: Seven years of white mold biocontrol product's performance efficacy on Sclerotinia sclerotiorum carpogenic germination in Brazil: a meta-analysis.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 9. | | MEDEIROS, F. H. V.; BETTIOL, W.; SOUZA, R. M. de; ALVES, E.; PINTO, Z. V.; IOST, R. Microorganisms, application timing and fractions as players of the milk-mediated powdery mildew management. Crop Protection, Surrey, v. 40, p. 8-15, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 11. | | MEDEIROS, F. H. V.; MARTINS, S. J.; ZUCCHI, T. D.; MELO, I. S. de; BATISTA, L. R.; MACHADO, J. da C. Biological control of mycotoxin-producing molds. Ciência e Agrotecnologia, Lavras, v. 36, n. 5, p. 483-497, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 12. | | FARIA, M. R. de; GUIMARÃES, R. A.; PINTO, F. A. M. F.; SIQUEIRA, C. da S.; SILVA, C. A.; MEDEIROS, F. H. V.; BETTIOL, W. Contribution of organic amendments to soil properties and survival of Stenocarpella on maize stalk. Scientia Agricola, Piracicaba, v. 77, n. 6, e20180033, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 13. | | TALAMINI, V.; CARVALHO, E. de A.; MEDEIROS, F. H. V. de; PEREIRA, P. de F.; OLIVEIRA, F. A. de; SANTOS, C. C. dos; SILVA, J. C. da. Prospecção e eficiência de antagonistas à resinose do coqueiro (Thielaviopsis paradoxa) nos Tabuleiros Costeiros e na Amazônia Oriental. In: SEMINÁRIO SOBRE INTENSIFICAÇÃO ECOLÓGICA DA FRUTICULTURA, 3.; REUNIÃO COMITÊ GESTOR DO PROJETO SISTEMA ECOLOGICAMENTE INTENSIVO DE PRODUÇÃO DE FRUTAS, 3., 2014, Aracaju. Seifrut: [anais..]. Brasília, DF: Embrapa, 2014. p. 172-176. Editores Técnicos: Carlos Roberto Martins, Francisco Alisson Xavier, Antônio Souza do Nascimento.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Tabuleiros Costeiros. |
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| 14. | | NUNES, P. S. de O.; JÚNIOR, G. V. L.; MASCARIN, G. M.; GUIMARÃES, R. A.; MEDEIROS, F. H. V. de; ARTHURS, S.; BETTIOL, W. Microbial consortia of biological products: do they have a future? Biological Control, v. 188, article 105439, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 15. | | MARTINS, S. A.; SCHURT, D. A.; SEABRA, S. S.; MARTINS, S. J.; RAMALHO, M. A. P.; MOREIRA, F. M. de S.; SILVA, J. C. P. da; SILVA, J. A. G. da; MEDEIROS, F. H. V. de. Common bean (Phaseolus vulgaris L.) growth promotion and biocontrol by rhizobacteria under Rhizoctonia solani suppressive and conducive soils. Applied Soil Ecology, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Roraima. |
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| 16. | | BUENO, A. de F.; CARVALHO. G. A.; NOGUEIRA, M. A.; MEDEIROS, F. H. V. de; MEDEIROS, F. C. L. de; HUNGRIA, M.; ARDISSON-ARAÚJO, D. M.; RIBEIRO, B. M.; SÓSA-GOMEZ, D. R.; HIROSE, E. Compatibilidade no uso de bioinsumos e insumos sintéticos no manejo da cultura da soja. In: MEYER, M. C.; BUENO, A. de F.; MAZARO, S. M.; SILVA, J. C. da (ed.). Bioinsumos na cultura da soja. Brasília, DF: Embrapa, 2022. cap. 27. p. 473-492.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Soja. |
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| 17. | | GUIMARÃES, R. A.; ZANOTTO, E.; ZANOTTO, L. A. S.; MACHADO, J. C.; MELO, I. S. de; VON PINHO, R. G.; PINTO, F. A. M. F.; FORTÊS, P. H. O. S.; MEDEIROS, H. N.; MEDEIROS, F. H. V. Integrating fungicide and biocontrol foliar spray on maize grain yield and fumonisin content. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 49., 2016, Maceió. Anais... Maceió: Sociedade Brasileira de Fitopatologia, 2016. Ref. 254.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 18. | | CARVALHO, A. L. de A.; REZENDE, L. C. de; COSTA, L. B.; HALFELD-VIEIRA, B. de A.; PINTO, Z. V.; MORANDI, M. A. B.; MEDEIROS, F. H. V.; BETTIOL, W. Optimizing the mass production of Clonostachys rosea by liquid-state fermentation. Biological Control, v. 118, p. 16-25, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 19. | | REZENDE, L. C. de; CARVALHO, A. L. de A.; COSTA, L. B.; HALFELD-VIEIRA, B. de A.; SILVA, L. G.; PINTO. Z. V.; MORANDI, M. A. B.; MEDEIROS, F. H. V. de; MASCARIN, G. M.; BETTIOL, W. Optimizing mass production of Trichoderma asperelloides by submerged liquid fermentation and its antagonism against Sclerotinia sclerotiorum. World Journal of Microbiology & Biotechnology, v. 36, n. 8, article 113, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 20. | | MEYER, M. C.; CAMPOS, H. D.; GODOY, C. V.; UTIAMADA, C. M.; OLIVEIRA, M. C. N. de; JACCOUD FILHO, D. de S.; VENANCIO, W. S.; MEDEIROS, F. H. V. de; JULIATTI, F. C.; CARNEIRO, L. C.; NUNES JUNIOR, J.; MARTINS, M. C. Experimentos cooperativos de controle biológico de Sclerotinia sclerotiorum na cultura da soja: Resultados sumarizados da safra 2018/2019. Londrina: Embrapa Soja, 2019. 5 p. (Embrapa Soja. Circular técnica, 155).| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 27 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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