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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
03/10/2008 |
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Data da última atualização: |
11/12/2023 |
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Autoria: |
SETH, J.; RUTHERFORD, F. J.; MCKENZIE, I. |
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Título: |
Solid-phase radioimmunoassay of thyroxine in untreated serum. |
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Ano de publicação: |
1975 |
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Fonte/Imprenta: |
Clinical Chemistry, v. 21, n. 10, p. 1406-1413, 1975. |
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Idioma: |
Inglês |
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Conteúdo: |
We describe a simple, convenient solid-phase radioimmunoassay of total thyroxine in unextracted serum. Serum samples are added directly to the assay incubation mixture, interference in the antigen/antibody reaction by the thyroxine-binding serum proteins being almost completely eliminated by the addition of 8-anilino-1-naphthalene sulfonic acid and incubation at pH 10.5. Residual interference is compensated for by including thyroxine-free serum in the standards. Use of thyroxine antibodies that are coupled to a solid support permits separation of free and antibody-bound hormone by a single washing step, followed by centrifugation. The method is specific, accurate, and reasonably precise. The results obtained compare well with those for radioimmunoassay of thyroxine in serum freed of protein by gel filtration, and with results of a competitive protein-binding method. The technical simplicity of the procedure should readily permit automation. These features suggest that the technique should be well suited for routine clinical laboratory use. |
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Palavras-Chave: |
Competitive protein binding; Glandula tireoide; Intermethod comparison; Pediatric chemistry; Radio imunizacao; Radioassay; Radioimunoensaio. |
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Thesagro: |
Hormônio; Reprodução Animal; Tiroxina. |
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Thesaurus Nal: |
Thyroid diseases. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01840naa a2200277 a 4500
001 1528203
005 2023-12-11
008 1975 bl uuuu u00u1 u #d
100 1 $aSETH, J.
245 $aSolid-phase radioimmunoassay of thyroxine in untreated serum.$h[electronic resource]
260 $c1975
520 $aWe describe a simple, convenient solid-phase radioimmunoassay of total thyroxine in unextracted serum. Serum samples are added directly to the assay incubation mixture, interference in the antigen/antibody reaction by the thyroxine-binding serum proteins being almost completely eliminated by the addition of 8-anilino-1-naphthalene sulfonic acid and incubation at pH 10.5. Residual interference is compensated for by including thyroxine-free serum in the standards. Use of thyroxine antibodies that are coupled to a solid support permits separation of free and antibody-bound hormone by a single washing step, followed by centrifugation. The method is specific, accurate, and reasonably precise. The results obtained compare well with those for radioimmunoassay of thyroxine in serum freed of protein by gel filtration, and with results of a competitive protein-binding method. The technical simplicity of the procedure should readily permit automation. These features suggest that the technique should be well suited for routine clinical laboratory use.
650 $aThyroid diseases
650 $aHormônio
650 $aReprodução Animal
650 $aTiroxina
653 $aCompetitive protein binding
653 $aGlandula tireoide
653 $aIntermethod comparison
653 $aPediatric chemistry
653 $aRadio imunizacao
653 $aRadioassay
653 $aRadioimunoensaio
700 1 $aRUTHERFORD, F. J.
700 1 $aMCKENZIE, I.
773 $tClinical Chemistry$gv. 21, n. 10, p. 1406-1413, 1975.
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Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
10/04/1992 |
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Data da última atualização: |
13/05/2014 |
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Autoria: |
FOLLE, S. M.; QUEIROGA, R. B.; FRANZ, C. A. B. |
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Título: |
Influencia do teor de agua no solo na demanda de tracao especifica de implementos de preparo de solo I. Arado de discos lisos e recortados. |
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Ano de publicação: |
1990 |
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Fonte/Imprenta: |
Planaltina: EMBRAPA-CPAC, 1990. |
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Páginas: |
3p. |
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Série: |
(EMBRAPA-CPAC. Pesquisa em Andamento, 34). |
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Idioma: |
Português |
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Palavras-Chave: |
Agricultural equipment; Agricultural tools; Brasil; Cerrados; Disc ploughs; Implementos agricolas; Preparo; Solos. |
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Thesagro: |
Agricultura; Água; Arado de Disco; Cerrado; Irrigação; Manejo do Solo; Maquina. |
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Thesaurus NAL: |
agriculture; irrigation; soil management. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/102146/1/pesq-34.pdf
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Marc: |
LEADER 01007nam a2200361 a 4500
001 1549374
005 2014-05-13
008 1990 bl uuuu u0uu1 u #d
100 1 $aFOLLE, S. M.
245 $aInfluencia do teor de agua no solo na demanda de tracao especifica de implementos de preparo de solo I. Arado de discos lisos e recortados.
260 $aPlanaltina: EMBRAPA-CPAC$c1990
300 $a3p.
490 $a(EMBRAPA-CPAC. Pesquisa em Andamento, 34).
650 $aagriculture
650 $airrigation
650 $asoil management
650 $aAgricultura
650 $aÁgua
650 $aArado de Disco
650 $aCerrado
650 $aIrrigação
650 $aManejo do Solo
650 $aMaquina
653 $aAgricultural equipment
653 $aAgricultural tools
653 $aBrasil
653 $aCerrados
653 $aDisc ploughs
653 $aImplementos agricolas
653 $aPreparo
653 $aSolos
700 1 $aQUEIROGA, R. B.
700 1 $aFRANZ, C. A. B.
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