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Registro Completo |
Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
14/06/2010 |
Data da última atualização: |
01/11/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
NOBILE, P. M.; QUECINI, V.; BAZZO, B.; QUITERIO, G.; MAZZAFERA, P.; COLOMBO, C. A. |
Afiliação: |
PAULA M. NOBILE, IAC; VERA MARIA QUECINI, CNPUV; BARBARA BAZZO, IAC; GABRIELA QUITERIO, IAC; PAULO MAZZAFERA, INSTITUTO DE BIOLOGIA - UNICAMP; CARLOS A. COLOMBO, IAC. |
Título: |
Transcriptional profile of genes involved in the byosynthesis of phytate and ferritin in coffea. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Journal of Agricultural and Food Chemistry, Washington DC, v. 58, n. 6, p. 3479-3487, mar. 2010. |
DOI: |
10.1021/jf9043088 |
Idioma: |
Inglês |
Conteúdo: |
The present work aimed to study the control of the biosynthesis of the antinutritional factor phytate and its associated Fe-rich protein family, ferritin, in coffee. Phytate has the ability to chelate Fe, making it unavailable to human absorption. The Coffea genome databases were queried for genes associated with phytate metabolism and ferritin genes. The genetic framework for phytate biosynthesis and its reverse pathway was identified in silico analyses and indicate that Coffea phosphatidyl inositol kinase and monophosphatase families play nonredundant roles in phytate metabolism. The transcriptional profiles of phytate biosynthesis key-genes MYO-INOSITOL(3)P1 SYNTHASE, two genes coding for PHOSPHATIDYL INOSITOL KINASE, and three FERRITIN genes were temporally evaluated by qPCR in coffee seeds from two crop locations, Adamantina-SP and Ouro-Fino-MG, the last one traditionally associated with high-quality coffee beverage grain. A targeted metabolome profile of phytic acid contents throughout three fruit maturation stages in association with the transcriptional analysis was also obtained. Taken together, our data indicate that the investigated local conditions did not cause significant alterations in phytate biosynthesis. Futhermore, the temporal transcriptional profiling revealed that candidate gene expression is regulated independently of phytate accumulation. In contrast, the expression profile of ferritin-unit genes is affected by environmental conditions and genetic background. The roles of the investigated genes are discussed concerning the quality of coffee beverage. MenosThe present work aimed to study the control of the biosynthesis of the antinutritional factor phytate and its associated Fe-rich protein family, ferritin, in coffee. Phytate has the ability to chelate Fe, making it unavailable to human absorption. The Coffea genome databases were queried for genes associated with phytate metabolism and ferritin genes. The genetic framework for phytate biosynthesis and its reverse pathway was identified in silico analyses and indicate that Coffea phosphatidyl inositol kinase and monophosphatase families play nonredundant roles in phytate metabolism. The transcriptional profiles of phytate biosynthesis key-genes MYO-INOSITOL(3)P1 SYNTHASE, two genes coding for PHOSPHATIDYL INOSITOL KINASE, and three FERRITIN genes were temporally evaluated by qPCR in coffee seeds from two crop locations, Adamantina-SP and Ouro-Fino-MG, the last one traditionally associated with high-quality coffee beverage grain. A targeted metabolome profile of phytic acid contents throughout three fruit maturation stages in association with the transcriptional analysis was also obtained. Taken together, our data indicate that the investigated local conditions did not cause significant alterations in phytate biosynthesis. Futhermore, the temporal transcriptional profiling revealed that candidate gene expression is regulated independently of phytate accumulation. In contrast, the expression profile of ferritin-unit genes is affected by environmental conditions and genetic back... Mostrar Tudo |
Palavras-Chave: |
Fator anti-nutricional; Ferritina; Fitato. |
Thesagro: |
Biologia; Café; Ferro; Genética. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/204172/1/12303-2010-p.3479-3487-2.pdf
|
Marc: |
LEADER 02404naa a2200277 a 4500 001 1854944 005 2019-11-01 008 2010 bl uuuu u00u1 u #d 024 7 $a10.1021/jf9043088$2DOI 100 1 $aNOBILE, P. M. 245 $aTranscriptional profile of genes involved in the byosynthesis of phytate and ferritin in coffea.$h[electronic resource] 260 $c2010 520 $aThe present work aimed to study the control of the biosynthesis of the antinutritional factor phytate and its associated Fe-rich protein family, ferritin, in coffee. Phytate has the ability to chelate Fe, making it unavailable to human absorption. The Coffea genome databases were queried for genes associated with phytate metabolism and ferritin genes. The genetic framework for phytate biosynthesis and its reverse pathway was identified in silico analyses and indicate that Coffea phosphatidyl inositol kinase and monophosphatase families play nonredundant roles in phytate metabolism. The transcriptional profiles of phytate biosynthesis key-genes MYO-INOSITOL(3)P1 SYNTHASE, two genes coding for PHOSPHATIDYL INOSITOL KINASE, and three FERRITIN genes were temporally evaluated by qPCR in coffee seeds from two crop locations, Adamantina-SP and Ouro-Fino-MG, the last one traditionally associated with high-quality coffee beverage grain. A targeted metabolome profile of phytic acid contents throughout three fruit maturation stages in association with the transcriptional analysis was also obtained. Taken together, our data indicate that the investigated local conditions did not cause significant alterations in phytate biosynthesis. Futhermore, the temporal transcriptional profiling revealed that candidate gene expression is regulated independently of phytate accumulation. In contrast, the expression profile of ferritin-unit genes is affected by environmental conditions and genetic background. The roles of the investigated genes are discussed concerning the quality of coffee beverage. 650 $aBiologia 650 $aCafé 650 $aFerro 650 $aGenética 653 $aFator anti-nutricional 653 $aFerritina 653 $aFitato 700 1 $aQUECINI, V. 700 1 $aBAZZO, B. 700 1 $aQUITERIO, G. 700 1 $aMAZZAFERA, P. 700 1 $aCOLOMBO, C. A. 773 $tJournal of Agricultural and Food Chemistry, Washington DC$gv. 58, n. 6, p. 3479-3487, mar. 2010.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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Registros recuperados : 29 | |
2. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FERREIRA, L. P.; GEROMEL, C.; CAVALAR, A. A.; MARRACCINI, P.; MAZZAFERA, P.; PEREIRA, L. F. P.; MAZZAFERA, P. Caracterização molecular de enzimas chave do metabolismo de açúcares em café. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 3., 2003, Porto Seguro. Resumos... Brasília, DF: Embrapa Café, 2003.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Café. |
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6. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | CONCEIÇÃO, H. E. O. da; MAZZAFERA, P.; ROCHA NETO, O. G. da; STEIN, R. L. B. Composição bioquímica e enzimas oxidativas em folhas de cupuaçuzeiro (Theobroma grandiflorum (Wildenow ex Sprengel) Schumann) infectadas por Crinipellis perniciosa. In: SEMINÁRIO INTERNACIONAL SOBRE PIMENTA-DO-REINO E CUPUAÇU, 1., 1996, Belém, PA. Resumos. Belém, PA: EMBRAPA-CPATU: JICA, 1996. p. 35. (EMBRAPA-CPATU. Documentos, 88).Biblioteca(s): Embrapa Amazônia Oriental. |
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7. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | CONCEIÇÃO, H. E. O. da; MAZZAFERA, P.; ROCHA NETO, O. G. da; STEINL, R. L. B. Composição bioquímica e enzimas oxidativas em folhas de cupuaçuzeiro (Theobroma grandiflorum (Willdenox ex Sprengel) Shumann) infectadas por Crinipellis perniciosa. In: SEMINÁRIO INTERNACIONAL SOBRE PIMENTA-DO-REINO E CUPUAÇU, 1996, Belém, PA. Anais... Belém, PA: Embrapa-CPATU: JICA, 1997. p. 173-181. (Embrapa-CPATU. Documentos, 89).Biblioteca(s): Embrapa Amazônia Oriental. |
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8. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MACHADO, M. A.; ÁSTUA, J. F.; AMARAL, A. M. do; MALOSSO, A.; MAZZAFERA, P. Gene expression of carotenoid biosynthesis pathway and carotenoid content in different varieties of sweet orange (Citrus sinensis L. Osb.). In: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan,China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 60. 105.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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10. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | NOBILE, P. M.; QUECINI, V.; BAZZO, B.; QUITERIO, G.; MAZZAFERA, P.; COLOMBO, C. A. Transcriptional profile of genes involved in the byosynthesis of phytate and ferritin in coffea. Journal of Agricultural and Food Chemistry, Washington DC, v. 58, n. 6, p. 3479-3487, mar. 2010.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Uva e Vinho. |
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12. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | GEROMEL, C.; MAZZAFERA, P.; MARRACCINI, P. R.; FERREIRA, L. P.; VIEIRA, L. G. E.; PEREIRA, L. F. P. Açúcares solúveis, sacarose sintase e sacarose fosfato sintase durante o desenvolvimento do fruto de café, sob diferentes condições In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia. Anais... Brasília, DF: Embrapa Café, 2007.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Café. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso ao objeto digital](/consulta/web/img/pdf.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
13. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | GEROMEL, C.; MAZZAFERA, P.; MARRACCINI, P. R.; FERREIRA, L. P.; VIEIRA, L. G. E.; PEREIRA, L. F. P. Açúcares solúveis, sacarose sintase e sacarose fosfato sintase durante o desenvolvimento do fruto de café, sob diferentes condições de luz e carga. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 4., 2005, Londrina. Anais... Brasília, DF: Embrapa Café, 2005.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Café. |
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14. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FELTRIM, D.; GUPTA, B.; GUNDIMEDA, S.; KIYOTA, E.; DOMINGUES JÚNIOR, A. P.; CINTRA, L. C.; MAZZAFERA, P. Exposure of Eucalyptus to varied temperature and CO2 has a profound effect on the physiology and expression of genes related to cell wall formation and remodeling. Tree Genetics & Genomes v. 18, n. 1, 3, Feb. 2022.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Agricultura Digital. |
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15. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | BOSCO, D. D.; FERNANDO, J. A.; MAZZAFERA, P.; MAIA, J. D. G.; RITSCHEL, P. S.; QUECINI, V. Distinct transcriptional networks control environment sensing and development in wild and cultivated grapevine genotypes. In: Congresso Brasileiro de Genética, 61., 2015, Águas Lindas de Lindóia, SP. Águas Lindas de Lindóia, SP: SBG, 2015.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Uva e Vinho. |
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16. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | GEROMEL, C.; FERREIRA, L. P.; GUERREIRO, S. M. C.; CAVALARI, A. A.; POT, D.; PEREIRA, L. F. P.; LEROY, T.; VIEIRA, L. G. E.; MAZZAFERA, P.; MARRACCINI, P. Biochemical and genomic analysis of sucrose metabolism during coffee (Coffea arabica) fruit development. Journal of Experimental Botany, v. 57, n. 12, p. 3243-3258, 2006.Tipo: Artigo em Periódico Indexado |
Biblioteca(s): Embrapa Café. |
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17. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SILVA, E. A. da; MAZZAFERA, P.; BRUNINI, O.; SAKAU, E.; ARRUDA, F. B.; MATTOSO, L. H. C.; CARVALHO, C. R. L. PIRES, R. C. M. The influence of water management and environmental conditions on the chemical composition and beverage quality of coffe beans. Brazilian Journal of Plant Physiology, Piracicaba, v. 17, n. 2, p. 229-238, June 2005.Biblioteca(s): Embrapa Instrumentação. |
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18. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | GEROMEL, C.; PEREIRA, L. P.; CAVALARI, A. A.; PEREIRA, L. F. P.; VIEIRA, L. G. E.; LEROY, T.; MAZZAFERA, P.; MARRACCINI, P. Metabolismo de açúcares durante o desenvolvimento de frutos de café. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 4., 2005, Londrina. Anais... Brasília, DF: Embrapa Café, 2005.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Café. |
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19. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | GEROMEL, C.; FERREIRA, L. P.; BOTTCHER, A.; POT, D.; PEREIRA, L. F. P.; LEROY, T.; VIEIRA, L. G. E.; MAZZAFERA, P.; MARRACCINI, P. Sucrose metabolism during fruit development in coffea racemosa. Annals of Applied Biology, v. 152, n. 2, p.179-187, 2008.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
Biblioteca(s): Embrapa Café. |
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20. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | GEROMEL, C.; FERREIRA, L. P.; CAVALARI, A. A.; PEREIRA, L. F. P.; VIEIRA, L. G. E.; LEROY, T.; MAZZAFERA, P.; MARRACCINI, P. Sugar metabolism during coffee fruit development. In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 20., 2004, Bangalore, India. Anais... Montpellier, France: Association for Science and Information on Coffee, 2005. 1 CD-ROM.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Café. |
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Registros recuperados : 29 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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